Fruits hidden by green: an improved YOLOV8n for detection of young citrus in lush citrus trees.

In order to address the challenges of inefficiency and insufficient accuracy in the manual identification of young citrus fruits during thinning processes, this study proposes a detection methodology using the you only look once for complex backgrounds of young citrus fruits (YCCB-YOLO) approach. The method first constructs a dataset containing images of young citrus fruits in a real orchard environment. To improve the detection accuracy while maintaining the computational efficiency, the study reconstructs the detection head and backbone network using pointwise convolution (PWonv) lightweight network, which reduces the complexity of the model without affecting the performance. In addition, the ability of the model to accurately detect young citrus fruits in complex backgrounds is enhanced by integrating the fusion attention mechanism. Meanwhile, the simplified spatial pyramid pooling fast-large kernel separated attention (SimSPPF-LSKA) feature pyramid was introduced to further enhance the multi-feature extraction capability of the model. Finally, the Adam optimization function was used to strengthen the nonlinear representation and feature extraction ability of the model. The experimental results show that the model achieves 91.79% precision (P), 92.75% recall (R), and 97.32% mean average precision (mAP)on the test set, which were improved by 1.33%, 2.24%, and 1.73%, respectively, compared with the original model, and the size of the model is only 5.4 MB. This study could meet the performance requirements for citrus fruit identification, which provides technical support for fruit thinning.

Chemical Constituents in Tibetan Medicine Dolomiaea Souliei (Franch.) Shih / 中国药师

Objective: To study the chemical constituents in the dry roots of Dolomiaea souliei (Franch.) Shih.Methods: Various chromatographic methods were used to isolate and purify the chemical constituents of Dolomiaea souliei, and the structures were elucidated through the analysis of spectral data and literatures.Results: Six compounds including 3 sesquiterpene compounds and 3 fatty acids were obtained and identified as dihydrodehydrocostuslactone(Ⅰ), vladimenal(Ⅱ), arbusculin A(Ⅲ), n-hendecane(Ⅳ), butanedioic acid(Ⅴ) and methyl linoleate(Ⅵ).Conclusion: Compounds Ⅳ-Ⅵ are obtained from the genus of Dolomiaea for the first time.

Hypolipidemic activity of total phenylpropanoid glycosides fromLigustrum robustum(Roxb.) Blume and its mechanisms on AMPK pathway / 中国药理学通报

Aim To evaluate the hypolipidemic effect of total phenylpropanoid glycoside extracted from Ligustrum robustum(Roxb.)Blume(CNTG)and its mecha-nisms.Methods The 60 hamsters were randomly divided into six groups,namely the control group,the model group,the positive control group(fenofibrate,150 mg·kg-1),the high(1 200 mg·kg-1),medium(600 mg·kg-1)and low(300 mg·kg-1)doses of CNTG groups.Only the control group was given control diet and other groups received high-fat diet.The changes of serum lipid were measured and analyzed in 1st week to ensure the successful establishment of the model.The drugs were administered daily for four weeks and the concentrations of lipids were determined in the 2nd week,3rd week and 4th week respectively.Quantitative real-time PCR and Western blot were used to assay the mRNA and protein expression of related signaling enzymes and proteins.Results Compared with the model group,the concentrations of serum TG,TC,LDL-C(P<0.05,P<0.01)and hepatic TG,TC(P<0.01)were effectively reduced in hamsters in CNTG-treated groups.Mechanism research found that CNTG increased the levels of phospho-AMPKα,LKB1 and phospho-LKB1 in liver(P<0.05).Conclusion CNTG prevents hyperlipidemia via activation of hepatic LKB1-AMPK pathway.

Research Progress in Cymbopogon citratus (DC.) Stapf and Lemongrass Tea / 世界科学技术-中医药现代化

C.citratus has been usedin many countries with a long history.Traditionally,it is applied as a food seasoning in cooking.It is also used in tea beverage and folk medicine as well.Modern application of C.citratus is focused on the development of citronella oil,which can be used for food additives,disinfectants,cosmetics,drugs and etc.C.citratus is also a potential plant in landscaping.Its special lemony flavor contains chemical constituents,mainly including citral,myrcene,linalool,geraniol,nerol,citronellol,and etc.The modern research showed that C.citratus had the main effects of anti-microbial,anti-inflammation,analgesia,anti-oxidation,anti-tumor,anti-anxiety,anti-hypertension,antihyperglycemia,and etc.With further studies,some new pharmacological properties of C.citrates are going to be discovered gradually.It is worthy of further research and development to meet the needs of the health industry.

Study and Comparison on HPLC Fingerprint ofPhyllanthus Urinaria Linn andPhyllanthus Amarus Linn / 世界科学技术-中医药现代化

This study was aimed to establish the HPLC fingerprint ofPhyllanthus urinaria Linn and Phyllanthusamarus Linn, in order to provide evidences for the study on material basis. Analysis was performed on an INDUSTRIES Epic C18 120A (5μm, 250 mm × 4.6 mm) column eluted with the acetonitrile (A) - water (0.1% phosphoric acid, V/V) gradient system as mobile phase. The wavelength was 254 nm and the flow rate was 1mL·min-1. The column temperature was 30℃. The injection volume was 10 μL. The results showed that the HPLC fingerprint ofPhyllanthus urinaria L. andPhyllanthus amarus L. were established. It was concluded that the method was simple, accurate and reproducible. This study provids experimental data for rapid quality identification and comprehensive evaluation ofPhyllanthus urinaria L. andPhyllanthus amarus L..

Quantitation of phlorizin and phloretin using an ultra high performance liquid chromatography-electrospray ionization tandem mass spectrometric method.

A sensitive and selective ultra high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS) method for the determination of phlorizin and phloretin in human plasma has been firstly developed. Samples were prepared after protein precipitation and analyzed on a C18 column interfaced with a triple quadrupole tandem mass spectrometer. Negative electrospray ionization was employed as the ionization source. The mobile phase consisted of acetonitrile-water (0.02% formic acid), using a gradient procedure. The analytes and internal standard dihydroquercetin were both detected by use of multiple reaction monitoring mode. The method was linear in the concentration range of 2.5-1000.0 ng/mL. The lower limit of quantification (LLOQ) was 2.5 ng/mL. The intra- and inter-day relative standard deviation across three validation runs over the entire concentration range was less than 9.2%. The accuracy determined at three concentrations was within ± 7.3% in terms of relative error. The total run time was 12.0 min. This assay offers advantages in terms of expediency, and suitability for the analysis of phlorizin and phloretin in various biological fluids.

Analysis on the Invention Patent of Traditional Medicines about Type-2 Diabetes Mellitus / 世界科学技术-中医药现代化

In this paper, the patent of traditional medicines of type-2 diabetes mellitus before 2014 were searched, totally 1 229 patent family information were obtained. The patent information including developing trends, geographic distribution, competitive agencies, technical focus, herbs preferences and so on were analyzed. China is in a leader position on the number of applying patent of using traditional medicine to treat type-2 diabetes mellitus, and the main applications were research institutes, universities and pharmaceutical enterprises. Although the views and theo-ries on treatment of type-2 diabetes mellitus are variety and abound, the most frequently used traditional medicines are tonics and heat-clearing. This article reflected the history, clinical treatment way, developments, application of the diabetes traditional drugs after a meta-analysis,efforts to guide a better understanding and further research.

Comparative Studies on Antioxidant Activities of Green Tea and Four Tree-type Non-Camellia Teas / 世界科学技术-中医药现代化

This article was aimed to compare the antioxidant activities of Green tea and four tree-type non-Camellia teas. The antioxidant activities of the water extract of Green tea, Eagle tea, Large-leaved Kuding tea, Shiya tea and Qingqianliu tea were evaluated with four different in vitro assays including 1,1-diphenyl-2-picryl-hydrazyl (DPPH), ferric-reducing antioxidant power (FRAP), ferrous ion-chelating (FIC) and β-carotene-linoleate bleaching assay. In addition, the polyphenol contents of different teas were estimated by the Folin-Ciocalteu assay. The results showed that Shiya tea had strong antioxidant activity; its FIC and FRAP abilities are better compared with Green tea. The Eagle tea and Large-leaved Kuding tea have better antioxidant activity. The FRAP activity of Large-leaved Kuding tea was better than green tea. The DPPH of Eagle tea and Large-leaved Kuding tea were slightly lower than Green tea, but higher than the butylated hydroxytoluene (BHT). The antioxidant ability of Qingqianliu tea was the lowest. It was concluded that Shiya tea, Eagle tea, Large-leaved Kuding tea had relatively high development value which re-quires in-depth research and promotion.

Determination method of muscone in rat intestinal perfusate by GC-MS/MS and its intestinal absorption kinetic characteristics in rats / 中国中药杂志

<p><b>OBJECTIVE</b>To establish the method for determining muscone in rat intestinal perfusate by GC-MS/MS and study its intestinal absorption kinetic characteristics in rats.</p><p><b>METHOD</b>The GC-MS/MS method was used to determine the content of muscone in rat intestinal circulation fluid. In situ intestinal circulation perfusion was adopted to study absorption kinetics of muscone in rats.</p><p><b>RESULT</b>Muscone was proved to be well absorbed in each section of small intestine. Its absorption rate constants (Ka) and the absorption rate (A) in the rat intestine showed duodenum > jejunum (P < 0.05) , duodenum > ileum (P < 0.01). Its Ka, A and t1/2 in rat small intestine was 0.990 h(-1) , 43.58% and 0.705h, respectively.</p><p><b>CONCLUSION</b>Muscone was well absorbed in each intestinal section, with duodenum better than jejunum (Ka, T1/2, P < 0.05) significantly better than ileum (Ka, T1/2, P < 0.01; A, P < 0.05). There is no obvious statistical difference between jejunum and ileum.</p>

New Collection of Crude Drugs in Chinese Pharmacopoeia 2010 Ⅲ.Kadsurae Caulis / 中草药·英文版

The dried cane of Kadsura interior (Kadsurae Caulis) is recorded in Chinese Pharmacopoeia 2010 as Dian Jixueteng for the treatment of rheumatism,irregular menstruation,and deficiency of Qi and blood.In this paper,morphological characteristics,chemical constituents,and pharmacological activities in the cane of K.interior were summarized.Moreover,some suggestions about application and quality control of Kadsurae Caulis were proposed in order to provide a theoretical basis for further scientific use.

Ethno-pharmacological investigation of Schisandraceae plants in China / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate and arrange the ethno-pharmacological information of Schisandraceae plants in China.</p><p><b>METHOD</b>The information was obtained by literature search, specimen inspection, field resource investigation and samples collection.</p><p><b>RESULT</b>Twenty-six Schisandraece plants (inlcuding 4 varieties) have been used as folk medicines in different regions of China, the identical medicinal parts of different species in one genus show the similar usage, action and indications, and different medicinal parts with different administrated ways show different action and indications.</p><p><b>CONCLUSION</b>The results provide reliable information for resource development and comprehensive utilization of Schisandraece plant resource in China.</p>

Comparison of green tea and four other kind of teas / 中国中药杂志

Nowadays, tea and the substitution (other kinds of tea) take an important status in the drinking, food, health product and the related industry. The most popular Green tea, Maté originated from South America, Large-leaved Kudingcha, Small-leaved Kudingcha, and the Eagle tea used civilian have many similarities on history, chemical constituents and biological activities. In this article, we summarized the similarities and the characteristics of the Green tea and the other four teas in order to provide scientific evidences for better development of the tea and substitutions.

A novel lignan dimer from stems of Kadsura heteroclita / 中国中药杂志

As a part of our search for bioactive material, the chemical investigation of the stems of Kadsura heteroclita led up to the isolation of a new lignan, heteroclitin R. The structure was elucidated on the basis of nuclear magnetic resonance spectroscopic data. Heteroclitin R was a new compound as a lignan dimer obtained from Schisandraceae family for the first time.

Chemical constituents from seeds of Paeonia suffruticosa / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents from seeds of Paeonia sufruticosa.</p><p><b>METHOD</b>Various chromatographic techniques were used to isolate and purify the constituents, their physico-chemical properties and spectral data were employed to elucidate their structures.</p><p><b>RESULT</b>Thirteen compounds were isolated and identified as: paeoniflorin (1), oxypaeoniflorin (2), 6'-O-beta-D-glucopyranosylalbiflorin (3), 8-debenzoylpaeoniflorin (4), 8-debenzoylpaeonidanin (5), 1-O-beta-D-glucopyranosylpaeonisuf-frone (6), 1-O-beta-D-ethyl-mannopyranoside (7), sucrose (8), luteolin (9), apigenin (10), benzoic acid (11) and 1-0-beta-D-(4-hydroxybenzoyl) glucose (12).</p><p><b>CONCLUSION</b>compounds 2, 4-6 were isolated from this plant for the first time, compounds 7 and 12 were isolated from the family of Paeoniaceae for the first time.</p>

Chemical Constituents and Biological Activities of Plants from the genus Ehretia Linn / 中草药·英文版

The plants of the genus Ehretia Linn. composed of about 50 species mainly distributed in tropical Asia and Africa.They have been used as folk medicines or traditional tea to treat various ailments in China for a long time. This contribution reviews the chemical constituents isolated from the plants of the genus Ehretia Linn. and related biological activities of these species in the past few decades. The compounds in the genus mainly belong to the classes of phenolic acids, flavonoids, benzoquinones, cyanogenetic glycosides, and fatty acids. The main biological activities include antioxidant, anti-inflammatory, antisnake venom, and anti-allergic activities.

Effective components against HIV-1 replicative enzymes isolated from plants / 药学学报

Plant active components characterized of many different structures and activities on multiple targets, have made them to be the important sources of inhibitors on HIV-1. For finding leading compounds with new structure against HIV-1, three key HIV-1 replicative enzymes (reverse transcriptase, protease and integrase) were used as screening models. The in vitro activities of 45 plant derived components isolated from Schisandraceae, Rutaceae and Ranunculaceae were reported. Within twelve triterpene components isolated, eight compounds were found to inhibit HIV-1 protease, in these eight active compounds, kadsuranic acid A (7) and nigranoic acid (8), inhibited both HIV-1 protease and integrase; Among fifteen lignans, meso-dihydroguaiaretic acid (15) and kadsurarin (16) were active on HIV-1 reverse transcriptase, and 4, 4-di(4-hydroxy-3-methoxyphenly)-2, 3-dimethylbutanol (13) active on HIV-1 integrase. All of the six alkaloids, seven flavones, and five others compounds were not active or only with low activities against HIV-1 replicative enzymes. Further studies of the triterpene components showing strong inhibitory activities on HIV-1 were warranted.

Argument on the correct Chinese name of genus Kadsura Kaempf.ex Juss / 药学学报

Family Schisandraceae is composed of two genera Schisandra and Kadsura, which are quite different in regard to the used part and medical function. Traditionally, the fruits of Schisandra plants were called 'Wuweizi' used as tonic for neurasthenia, antitussive, and sedative agents. The stems and roots of Kadsura plants, with names related to 'XueTeng', possess activating blood circulation and eliminating stasis and are used for the treatment of rheumatism, fractures and irregular menstruation. The dried fruit of Schisandra chinensis was recorded in Chinese Pharmacopoeia (2010 Edition) as 'Wuweizi', while the dried fruit of S. sphenanthera was recorded as an official origin of 'Nanwuweizi'. Historically, there was no evidence that the fruits of Kadsura plants were substituted as 'Wuweizi' either in ancient literatures or contemporary marketing. However, genus Kadsura is still popularly called 'Nanwuweizi' and plant K. longipedunculata is regarded as the origin of 'Nanwuweizi', thus this will cause confusion as well as misunderstanding of genus Kadsura. The authors recommended, therefore, the Chinese name of genus Kadsura should be 'Lengfantengshu' and K. longipedunculata with the name of 'Lengfanteng' in order to guarantee the drug authenticity.

Chemical study on ethyl acetate soluble portion of Kadsura oblongifolia / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents in the stems of Kadsura oblongifolia.</p><p><b>METHOD</b>The chemical constituents were isolated by various column chromatographic methods. The structures were identified by spectral data.</p><p><b>RESULT</b>Seven compounds named kaempferol-3-O-alpha-L-arabinofuranoside (1), kaempferol-3-O-alpha-D-arabinopyranoside (2), quercetin-3-O-alpha-D-arabinopyranoside (3), quercetin-3-O-alpha- L-arabinofuranoside (4), quercetin-3-O-beta-D-glucopyranoside (5), quercetin (6), kaempferol (7) were isolated and identified.</p><p><b>CONCLUSION</b>Compounds 1-7 were obtained from this plant for the first time. Compounds 1-4 were isolated from Schisandraceae family for the first time.</p>

Triterpenes and triterpene glycosides from aerial part of Paraboea glutinosa / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents from aerial part of Paraboea glutinosa.</p><p><b>METHOD</b>The compounds were isolated with silica gel, Sephadex LH-20 column chromatography and their structures were elucidated by means of spectral data analysis.</p><p><b>RESULT</b>Five compounds were isolated and identified as 2alpha, 3beta, 19alpha, 24-tetrahydroxyurs-12-en-28-oate(24-hydroxytormentic acid,1), glucosyl-2alpha, 3beta, 19alpha, 24-tetrahydroxyurs-12-en-28-oate (24-hydroxytormentic acid ester glucoside,2), 28-O-beta-D-glucopyranosyl (1-->6)-beta-D-glucopyranosyl-24-hydroxytormentic acid (3), beta-sitosterol (4), daucosterol (5).</p><p><b>CONCLUSION</b>All these compounds were isolated from the genus Paraboea for the first time.</p>

Chemical study on petroleum ether portion of Sarcandra hainanensis / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents in the whole plants of Sarcandra hainanensis.</p><p><b>METHOD</b>The chemical constituents were isolated by various column chromatographic methods. The structures were identified by spectral data.</p><p><b>RESULT</b>Nine compounds, palmitic acid (1), icosanoic acid (2), beta-sitosterol (3), octadecanoic (4), acidchrysophanol (5), emodin (6), 2', 3'-dihydroxy-4', 6'-dimethoxychalcone (7), 2'-hydroxy4', 6'-dimethoxychalcone (8), cardamonin (9) were isolated and identified.</p><p><b>CONCLUSION</b>Compounds 1-9 were obtained from this plant for the first time. Compounds 2, 5-9 were isolated from family Chloranthaceae for the first time.</p>