RESUMEN
The global emergency caused by the SARS-CoV-2 pandemics can only be solved with adequate preventive and therapeutic strategies, both currently missing. The electropositive Receptor Binding Domain (RBD) of SARS-CoV-2 spike protein with abundant {beta}-sheet structure serves as target for COVID-19 therapeutic drug design. Here, we discovered that ultrathin 2D CuInP2S6 (CIPS) nanosheets as a new agent against SARS-CoV-2 infection, which also able to promote viral host elimination. CIPS exhibits extremely high and selective binding capacity with the RBD of SARS-CoV-2 spike protein, with consequent inhibition of virus entry and infection in ACE2-bearing cells and human airway epithelial organoids. CIPS displays nano-viscous properties in selectively binding with spike protein (KD < 1 pM) with negligible toxicity in vitro and in vivo. Further, the CIPS-bound SARS-CoV-2 was quickly phagocytosed and eliminated by macrophages, suggesting CIPS could be successfully used to capture and facilitate the virus host elimination with possibility of triggering anti-viral immunization. Thus, we propose CIPS as a promising nanodrug for future safe and effective anti-SARS-CoV-2 therapy, as well as for use as disinfection agent and surface coating material to constrain the SARS-CoV-2 spreading.
RESUMEN
[Objective]We summarize the Professor DONG Youqi's clinical experience in treating infantile virus enteritis in the medium term(the syndrome of Relian Shangyin) with Shengqing Yunpi method.[Method]The paper analyzes the etiology and pathogenesis of infantile virus enteritis in the medium term and describes the criteria of Shengqing Yunpi method in clinical application. We analyze the component, meaning and function of empirical prescription called"Shengqing Yunpi decoction", then give clinical cases to verify. [Results]In his view, the etiology and pathogenesis of the syndrome of Relian Shangyin is basically caused by the pathogenic factors of heat -dampness and body weakness, causing damp -heat retention, disease deferment and yin deficiency. According to its etiology and pathogenesis, Professor DONG adopts the method of Yang-Yin-Yun-Pi and Sheng-Qing-Jiang-Zhuo and the experienced prescription of Shengqing Yunpi decoction to treat the disease.This method is consistent with the pathology of the disease and is also helpful for the overall recovery of children's spleen and stomach function. [Conclusion]The method of Shengqing Yunpi is effective in treating infantile virus enteritis of the syndrome of Relian Shangyin and is worth learning and imitating.
