Asunto(s)
Infecciones Urinarias/microbiología , Orina/microbiología , Escherichia coli Uropatógena/aislamiento & purificación , beta-Lactamasas/genética , beta-Lactamasas/metabolismo , Anciano , Análisis del Polimorfismo de Longitud de Fragmentos Amplificados , Análisis por Conglomerados , Femenino , Genes Bacterianos , Genotipo , Humanos , Pruebas de Sensibilidad Microbiana , Tipificación Molecular , Reacción en Cadena de la Polimerasa , República de Corea , Análisis de Secuencia de ADN , Escherichia coli Uropatógena/clasificación , Escherichia coli Uropatógena/enzimología , Escherichia coli Uropatógena/genética , Resistencia betalactámicaAsunto(s)
Infecciones por Acinetobacter/epidemiología , Acinetobacter baumannii/enzimología , Infección Hospitalaria/epidemiología , Farmacorresistencia Bacteriana Múltiple , beta-Lactamasas/metabolismo , Infecciones por Acinetobacter/microbiología , Acinetobacter baumannii/clasificación , Acinetobacter baumannii/genética , Acinetobacter baumannii/aislamiento & purificación , Análisis del Polimorfismo de Longitud de Fragmentos Amplificados , Antibacterianos/farmacología , Análisis por Conglomerados , Infección Hospitalaria/microbiología , Humanos , Pruebas de Sensibilidad Microbiana , Epidemiología Molecular , Tipificación Molecular , Reacción en Cadena de la Polimerasa , Prevalencia , República de Corea/epidemiología , beta-Lactamasas/genéticaRESUMEN
A total of 100 clinical isolates of Escherichia coli (n = 35), Klebsiella pneumoniae (n = 63), Proteus mirabilis (n = 1), and Salmonella serovar Stanley (n = 1), showing resistance to cefoxitin, or returning positive in extended-spectrum ß-lactamase (ESBL) by Clinical and Laboratory Standards Institute (CLSI) ESBL confirmatory method, were studied. The isolates were examined by the boronic acid (BA) disk test, polymerase chain reaction, and pulsed-field gel electrophoresis (PFGE) to investigate genetic similarities. The concurrence rates for ESBLs by the CLSI and the BA disk test were 97% for E. coli and 96.7% for K. pneumoniae. A total of 41 isolates showing cefoxitin resistance yielded all positive by the BA disk test. All the 33 K. pneumoniae isolates, which showed positive by the BA disk test, were carrying AmpC genes. The TEM and CTX-M types were predominant in E. coli and the SHV and the CIT and/or DHA types were predominant in K. pneumoniae. PFGE analysis showed almost 75% of genetic similarities among K. pneumoniae isolates producing ESBLs and/or AmpC ß-lactamases (AmpCs) as each K. pneumoniae carried variable genes and showed variable antibiotic patterns. Clearly, the BA disk test was a useful method for the detection of ESBLs and AmpCs. In particular, cefoxitin resistance and BA-positive trait of K. pneumoniae do reflect the presence of AmpC genes in the organism.
Asunto(s)
Proteínas Bacterianas/metabolismo , Escherichia coli/enzimología , Klebsiella pneumoniae/enzimología , Proteus mirabilis/enzimología , Salmonella/enzimología , beta-Lactamasas/metabolismo , Proteínas Bacterianas/biosíntesis , Proteínas Bacterianas/genética , ADN Bacteriano/química , ADN Bacteriano/genética , Farmacorresistencia Bacteriana Múltiple , Electroforesis en Gel de Campo Pulsado , Escherichia coli/efectos de los fármacos , Escherichia coli/genética , Escherichia coli/aislamiento & purificación , Infecciones por Bacterias Gramnegativas/microbiología , Humanos , Klebsiella pneumoniae/efectos de los fármacos , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/aislamiento & purificación , Pruebas de Sensibilidad Microbiana , Reacción en Cadena de la Polimerasa , Prevalencia , Proteus mirabilis/efectos de los fármacos , Proteus mirabilis/genética , Proteus mirabilis/aislamiento & purificación , República de Corea , Salmonella/efectos de los fármacos , Salmonella/genética , Salmonella/aislamiento & purificación , beta-Lactamasas/biosíntesis , beta-Lactamasas/genéticaRESUMEN
Various tyrosine phosphorylation motif regions of H. pylori cagA exist. The number of these regions was found to have some influence on cell signaling, which was found to be more pronounced when in D (ESS) region than in C (WSS) region. A molecular biological method with multiplex PCR was developed to distinguish C and D regions, and to identify the repetition number of tyrosine phosphorylation of the cagA gene. Multiplex PCR using novel primer sets was performed on 73 strains of H. pylori isolated from Korean patients with upper gastrointestinal diseases. The Western cagA was identified in only 3 strains (4.1%) whereas East Asia cagA was identified in 69 strains (94.5%). These results were reconfirmed through a sequencing analysis. The method developed in this study would be useful for monitoring the repeated number of C and D regions of tyrosine phosphorylation motifs in H. pylori cagA.
