Inducing ovulation with oestradiol cypionate allows flexibility in the timing of insemination and removes the need for gonadotrophin-releasing hormone in timed AI protocols for dairy cows.

The effects of addition of gonadotrophin-releasing hormone (GnRH) to a progesterone plus oestradiol-based protocol and timing of insemination in Holstein cows treated for timed AI (TAI) were evaluated. Cows (n=481) received a progesterone device and 2mg oestradiol benzoate. After 8 days, the device was removed and 25mg dinoprost was administered. Cows were allocated to one of three (Study 1; n=57) or four (Study 2; n=424) groups, accordingly to ovulation inducer alone (Study 1; oestradiol cypionate (EC), GnRH or both) or ovulation inducer (EC alone or combined with GnRH) and timing of insemination (48 or 54h after device removal; Study 2). In Study 1, the diameter of the ovulatory follicle was greater for GnRH than EC. Oestrus and ovulation rates were similar regardless of ovulatory stimuli. However, time to ovulation was delayed when GnRH only was used. In Study 2, cows treated with GnRH or not had similar pregnancy per AI (P/AI) 30 days (41.5% vs 37.3%; P=0.28) and 60 days (35.9% vs 33.0%; P=0.61) after TAI. TAI 48 and 54h after device removal resulted similar P/AI at 30 days (40.3% vs 38.5%; P=0.63) and 60 days (33.8% vs 35.1%; P=0.72). Thus, adding GnRH at TAI does not improve pregnancy rates in dairy cows receiving EC. The flexibility of time to insemination enables TAI of a large number of cows using the same protocol and splitting the time of AI.

XDS: a flexible beamline for X-ray diffraction and spectroscopy at the Brazilian synchrotron.

The majority of the beamlines at the Brazilian Synchrotron Light Source Laboratory (LNLS) use radiation produced in the storage-ring bending magnets and are therefore currently limited in the flux that can be used in the harder part of the X-ray spectrum (above ∼10 keV). A 4 T superconducting multipolar wiggler (SCW) was recently installed at LNLS in order to improve the photon flux above 10 keV and fulfill the demands set by the materials science community. A new multi-purpose beamline was then installed at the LNLS using the SCW as a photon source. The XDS is a flexible beamline operating in the energy range between 5 and 30 keV, designed to perform experiments using absorption, diffraction and scattering techniques. Most of the work performed at the XDS beamline concentrates on X-ray absorption spectroscopy at energies above 18 keV and high-resolution diffraction experiments. More recently, new setups and photon-hungry experiments such as total X-ray scattering, X-ray diffraction under high pressures, resonant X-ray emission spectroscopy, among others, have started to become routine at XDS. Here, the XDS beamline characteristics, performance and a few new experimental possibilities are described.

Influence of the Nd:YAG laser pulse duration on the temperature of primary enamel.

The aim of this study is to evaluate the temperature change on specimens of primary enamel irradiated with different pulse duration of Nd:YAG laser. Fifteen sound primary molars were sectioned mesiodistally, resulting in 30 specimens (3.5 × 3.5 × 2.0 mm). Two small holes were made on the dentin surface in which K-type thermocouples were installed to evaluate thermal changes. Specimens were randomly assigned in 3 groups (n = 10): A = EL (extra long pulse, 10.000 µs), B = LP (long pulse, 700 µs), and C = SP (short pulse, 350 µs). Nd:YAG laser (λ = 1.064 µm) was applied at contact mode (10 Hz, 0.8 W, 80 mJ) and energy density of 0.637 mJ/mm(2). Analysis of variance (ANOVA) was performed for the statistical analysis (P = 0.46). Nd:YAG laser pulse duration provided no difference on the temperature changes on primary enamel, in which the following means were observed: A = EL (23.15°C ± 7.75), B = LP (27.33°C ± 11.32), and C = SP (26.91°C ± 12.85). It can be concluded that the duration of the laser pulse Nd:YAG increased the temperature of the primary enamel but was not influenced by different pulse durations used in the irradiation.

SLC24A5 and ASIP as phenotypic predictors in Brazilian population for forensic purposes.

Pigmentation is a variable and complex trait in humans and it is determined by the interaction of environmental factors, age, disease, hormones, exposure to ultraviolet radiation and genetic factors, including pigmentation genes. Many polymorphisms of these genes have been associated with phenotypic diversity of skin, eyes and hair color in homogeneous populations. Phenotype prediction from biological samples using genetic information has benefited forensic area in some countries, leading some criminal investigations. Herein, we evaluated the association between polymorphisms in the genes SLC24A5 (rs1426654) and ASIP (rs6058017) with skin, eyes and hair colors, in 483 healthy individuals from Brazilian population for attainable use in forensic practice. The volunteers answered a questionnaire where they self-reported their skin, eye and hair colors. The polymorphic homozygous genotype of rs1426654∗A and rs6058017∗A in SLC24A5 and ASIP respectively, showed strongest association with fairer skin (OR 47.8; CI 14.1-161.6 and OR 8.6; CI 2.5-29.8); SLC24A5 alone showed associations with blue eyes (OR 20.7; CI 1.2-346.3) and blond hair (OR 26.6; CI 1.5-460.9). Our data showed that polymorphic genotypes (AA), in both genes, are correlated with characteristics of light pigmentation, while the ancestral genotype (GG) is related to darker traits, corroborating with previous studies in European and African populations. These associations show that specific molecular information of an individual may be useful to access some phenotypic features in an attempt to help forensic investigations, not only on crime scene samples but also in cases of face reconstructions in unknown bodies.

Ações de vigilância para influenza aviária desenvolvida no Brasil, no período de 2004 e 2007 / Avian influenza surveillance activities, developed in Brazil from 2004 to 2007

No início dos anos 2000, o Brasil se consolidou como maior exportador mundial de carne de frango, mesmo período em que se observou a emergência global de focos de HPAI. Para assegurar a qualidade sanitária do produto avícola nacional, o Mapa organizou um programa oficial de vigilância para o vírus de IA. Na primeira fase, foram coletados 106.226 soros e 7.017 pools de suabes traqueais e cloacais, provenientes de granjas avícolas de produção comercial intensiva de frangos de corte, no período entre janeiro de 2004 e março de 2005. Não se obteve isolamento viral para IA, porém foi identificado um conglomerado epidemiológico de 24 municípios, delineado pela interpolação de dados relacionados às localizações geográficas, e os resultados sorológicos das amostras dessas origens, ELISA reagente para IA, no estado de Rondônia. Na segunda fase da vigilância, três distintas ações para pesquisa do vírus de IA foram executadas entre 2006 e 2007, em: 1) aves comerciais de corte de criação intensiva; 2) aves de reprodução; e 3) aves migratórias e de subsistência. Não houve identificação de resposta sorológica ou isolamento de vírus de IA em aves dos grupos 1 e 2. Foram isolados vírus de IA do subtipo H3 em aves migratórias, capturadas nos estados de Pará e Pernambuco. Também foram identificados vírus de IA dos subtipos H2, H3 e H4 em aves de subsistência, de propriedades localizadas no Amazonas, Pará, Pernambuco, Rio Grande do Sul e Santa Catarina. Observou-se risco sanitário para LPAI associado às populações de aves silvestres e de subsistência localizadas em áreas próximas ao sistema comercial. Estudos adicionais serão necessários para se avaliar o risco associado à introdução de IA no sistema comercial avícola brasileiro.

In the early 2000s Brazil had established itself as the world's largest exporter of poultry meat, the same period in which the emergence of HPAI global outbreaks was observed. To ensure the national health quality of Brazilian poultry product, MAPA organized an official AI surveillance program. In the first stage, during the period between January 2004 and March 2005, 106.226 sera were collected and 7.017 tracheal and cloacae pools of swabs were obtained from intensive commercial broiler farms. No AI virus isolation was obtained, however, an epidemiological cluster was identified in the state of Rondonia, outlined by the interpolation of data related to municipalities' geographic location and serological response to AI in ELISA tests. Between 2006 and 2007, during the second stage, three AI surveillance actions were executed in: 1) intensive commercial broiler farms, 2) breeding farms and 3) migratory and backyard birds. There was neither serological response identification nor IA virus isolation in birds belonging to groups 1 and 2. H3 LPAI subtype viruses were isolated from migratory birds captured in the states of Pará and Pernambuco. H2, H3 and H4 LPAI subtypes were also identified in backyard birds from samples collected in the states of Amazonas, Pará, Pernambuco, Rio Grande do Sul and Santa Catarina. There are health risks to LPAI associated to wild and backyard bird populations located in areas close to commercial farms. Additional studies are needed for risk assessment regarding the possibility of AI introduction in the Brazilian commercial poultry system.

Ações de vigilância para influenza aviária desenvolvida no Brasil, no período de 2004 e 2007 / Avian influenza surveillance activities, developed in Brazil from 2004 to 2007

No início dos anos 2000, o Brasil se consolidou como maior exportador mundial de carne de frango, mesmo período em que se observou a emergência global de focos de HPAI. Para assegurar a qualidade sanitária do produto avícola nacional, o Mapa organizou um programa oficial de vigilância para o vírus de IA. Na primeira fase, foram coletados 106.226 soros e 7.017 pools de suabes traqueais e cloacais, provenientes de granjas avícolas de produção comercial intensiva de frangos de corte, no período entre janeiro de 2004 e março de 2005. Não se obteve isolamento viral para IA, porém foi identificado um conglomerado epidemiológico de 24 municípios, delineado pela interpolação de dados relacionados às localizações geográficas, e os resultados sorológicos das amostras dessas origens, ELISA reagente para IA, no estado de Rondônia. Na segunda fase da vigilância, três distintas ações para pesquisa do vírus de IA foram executadas entre 2006 e 2007, em: 1) aves comerciais de corte de criação intensiva; 2) aves de reprodução; e 3) aves migratórias e de subsistência. Não houve identificação de resposta sorológica ou isolamento de vírus de IA em aves dos grupos 1 e 2. Foram isolados vírus de IA do subtipo H3 em aves migratórias, capturadas nos estados de Pará e Pernambuco. Também foram identificados vírus de IA dos subtipos H2, H3 e H4 em aves de subsistência, de propriedades localizadas no Amazonas, Pará, Pernambuco, Rio Grande do Sul e Santa Catarina. Observou-se risco sanitário para LPAI associado às populações de aves silvestres e de subsistência localizadas em áreas próximas ao sistema comercial. Estudos adicionais serão necessários para se avaliar o risco associado à introdução de IA no sistema comercial avícola brasileiro.(AU)

In the early 2000s Brazil had established itself as the world's largest exporter of poultry meat, the same period in which the emergence of HPAI global outbreaks was observed. To ensure the national health quality of Brazilian poultry product, MAPA organized an official AI surveillance program. In the first stage, during the period between January 2004 and March 2005, 106.226 sera were collected and 7.017 tracheal and cloacae pools of swabs were obtained from intensive commercial broiler farms. No AI virus isolation was obtained, however, an epidemiological cluster was identified in the state of Rondonia, outlined by the interpolation of data related to municipalities' geographic location and serological response to AI in ELISA tests. Between 2006 and 2007, during the second stage, three AI surveillance actions were executed in: 1) intensive commercial broiler farms, 2) breeding farms and 3) migratory and backyard birds. There was neither serological response identification nor IA virus isolation in birds belonging to groups 1 and 2. H3 LPAI subtype viruses were isolated from migratory birds captured in the states of Pará and Pernambuco. H2, H3 and H4 LPAI subtypes were also identified in backyard birds from samples collected in the states of Amazonas, Pará, Pernambuco, Rio Grande do Sul and Santa Catarina. There are health risks to LPAI associated to wild and backyard bird populations located in areas close to commercial farms. Additional studies are needed for risk assessment regarding the possibility of AI introduction in the Brazilian commercial poultry system.(AU)

Catechin attenuates behavioral neurotoxicity induced by 6-OHDA in rats.

This study was designed to investigate the beneficial effect of catechin in a model of Parkinson's disease. Unilateral, intrastriatal 6-hydroxydopamine (6-OHDA)-lesioned rats were pretreated with catechin (10 and 30 mg/kg) by intraperitoneal (i.p.) injection 2h before surgery and for 14 days afterwards. After treatments, apomorphine-induced rotations, locomotor activity, working memory and early and late aversive memories were evaluated. The mesencephalon was used to determine the levels of monoamines and measurement of glutathione (GSH). Immunohistochemical staining was also used to evaluate the expression of tyrosine hydroxylase (TH) in mesencephalic and striatal tissues. Catechin administration attenuated the increase in rotational behavior and the decrease in locomotor activity observed in lesioned rats. Although catechin did not rescue the impairment of late aversive memory, it protected the animals against 6-OHDA-induced working memory deficits. Furthermore, catechin treatment restored GSH levels, and significantly increased dopamine and DOPAC content, and TH-immunoreactivity in 6-OHDA-lesioned rats. Catechin protected 6-OHDA-lesioned rats due to its antioxidant action, indicating that it could be useful as an adjunctive therapy for the treatment of Parkinson's disease.

Efeitos da suplementação com fósforo em caprinos no semiárido do nordeste brasileiro / Effect of phosphorus supplementation for goats in the Brazilian semiarid region

To evaluate the effect of phosphorus supplementation for goats grazing for the semiarid region, one group of 16 recently weaned Moxotó goats was supplemented with a mineral supplement containing Na, Cl, Zn, Cu, Se, Co, and P during 240 days. Another similar group was supplemented with a similar mineral supplement without P. The mean daily consumption of supplement by animal was of 7.09±2.77g and 7.67±3.14g for the groups with and without P, respectively. The mean weight gain of the P supplemented group (45.20±5.56g) was significantly higher (P<0.05) than the non-supplemented group (40.03±2.80g). The average total P in soil was 30.8mg/kg and in the pasture 0.13 percent in dry matter. These results demonstrate the occurrence of P deficiency in some areas of the Brazilian semiarid region.

Efeitos da suplementação com fósforo em caprinos no semiárido do nordeste brasileiro / Effect of phosphorus supplementation for goats in the Brazilian semiarid region

To evaluate the effect of phosphorus supplementation for goats grazing for the semiarid region, one group of 16 recently weaned Moxotó goats was supplemented with a mineral supplement containing Na, Cl, Zn, Cu, Se, Co, and P during 240 days. Another similar group was supplemented with a similar mineral supplement without P. The mean daily consumption of supplement by animal was of 7.09±2.77g and 7.67±3.14g for the groups with and without P, respectively. The mean weight gain of the P supplemented group (45.20±5.56g) was significantly higher (P<0.05) than the non-supplemented group (40.03±2.80g). The average total P in soil was 30.8mg/kg and in the pasture 0.13 percent in dry matter. These results demonstrate the occurrence of P deficiency in some areas of the Brazilian semiarid region.(AU)

Head hair reduces sweat rate during exercise under the sun.

The purpose of this study was to evaluate the effects of human head hair on thermoregulation during exercise carried out under solar radiation. 10 healthy male subjects (mean±SD: 25.1±2.5 yr; height: 176.2±4.0 cm; weight: 73.7±6.7 kg; VO(2max) 56.2±5.3 mLO(2)·kg (-1)·min (-1)) took part in 2 1 h-long trials of continuous exercise on a treadmill at 50% VO2(max) under solar radiation that were separated by at least 2 days. Whereas for the first trial they retained their natural head hair (HAIR), in the second trial their hair was totally shaved (NOHAIR). Several properties were measured, including environmental heat stress (Wet Bulb Globe Temperature index - WBGT, °C), heart rate, rectal temperature, skin temperature, head temperature, and global sweat rate. The main findings were that whereas there was a lower sweat rate in the HAIR condition (HAIR: 7.08±0.79 vs. NOHAIR: 7.67±0.79 g·m (-2)·min (-1); p=0.03), there were no significant differences in any of the other variables between the HAIR and NOHAIR trials. In conclusion, the presence of head hair resulted in a lower sweat rate.

Effects of equine chorionic gonadotropin and type of ovulatory stimulus in a timed-AI protocol on reproductive responses in dairy cows.

The objectives were to evaluate the effects of equine chorionic gonadotropin (eCG) supplementation (with or without eCG) and type of ovulatory stimulus (GnRH or ECP) on ovarian follicular dynamics, luteal function, and pregnancies per AI (P/AI) in Holstein cows receiving timed artificial insemination (TAI). On Day 0, 742 cows in a total of 782 breedings, received 2mg of estradiol benzoate (EB) and one intravaginal progesterone (P4) insert (CIDR). On Day 8, the CIDR was removed, and all cows were given PGF2 alpha and assigned to one of four treatments in a 2 x 2 factorial arrangement: (1) CG: GnRH 48 h later; (2) CE: ECP; (3) EG: eCG+GnRH 48 h later; (4) EE: eCG+ECP. There were significant interactions for eCG x ovulatory stimulus and eCG x BCS. Cows in the CG group were less likely (28.9% vs. 33.8%; P<0.05) to become pregnant compared with those in the EG group (odds ratio [OR]=0.28). There were no differences in P/AI between CE and EE cows (30.9% vs. 29.1%; OR=0.85; P=0.56), respectively. Thinner cows not receiving eCG had lower P/AI than thinner cows receiving eCG (15.2% vs. 38.0%; OR=0.20; P<0.01). Treatment with eCG tended to increase serum progestesterone concentrations during the diestrus following synchronized ovulation (P<0.10). However, the treatment used to induce ovulation did not affect CL volume or serum progesterone concentrations. In conclusion, both ECP and GnRH yielded comparable P/AI. However, eCG treatment at CIDR removal increased pregnancy rate in cows induced to ovulate with GnRH and in cows with lower BCS.

UV laser-induced desorption mechanism analyzed through two-layer alkali halide samples.

Time of flight-mass spectrometry (TOF-MS) is used to analyze positive and negative desorbed ions generated by UV laser ablation of several alkali (X) halide (Y) salts. Most of the observed desorbed cluster ions have the structure (XY)(n)X(+) or (XY)(n)Y(-). Their desorption yields decrease as exp(-kn), where k approximately 2 for both series, suggesting that the neutral component (XY)(n) plays the dominant role in the desorption process. Mass spectrum measurements were performed for compound samples in which two salts (out of CsI, RbI, KBr, KCl and KI) are homogeneously mixed or disposed in two superposed layers. The detection of small new ion species and large cluster ions of the original salts supports the scenario that the uppermost layers are completely atomized while deep layers are emitted colder and fragmented: It is proposed that ns-pulsed laser induced desorption of ionic salts occurs via two sequential mechanisms: (1) ejection of cations and anions in the hot plume, followed by recombination into new cluster ions and (2) ejection of relatively cold preformed species originated from deep layers or from periphery of the irradiated region.

Effects of gamma radiation on phase behaviour and critical micelle concentration of Triton X-100 aqueous solutions.

Ionising radiation used for sterilization can have an effect on the physicochemical properties of pharmaceutically relevant excipient systems, affecting therefore the stability of the formulation. The effect of gamma irradiation on the phase behaviour (cloud point--CP) and critical micelle concentration (CMC) of aqueous solutions of Triton X-100, used as a model nonionic surfactant, is investigated in this paper. Micellar solutions were irradiated with gamma-rays in a dose range between 0 and 70 kGy, including the sterilization range of pharmaceutical preparations. The decreased observed in CP and CMC values of micellar solutions at all absorbed doses was explained in terms of changes in molecular mass distribution of ethoxylated surfactant and the formation of cross-linked species. These results were complemented by mass spectrometry, UV-vis and NMR spectroscopy. Although the findings indicate degradation of polyethoxylated chains by water radical attacks, there was no spectroscopic evidence of radiation damage to aromatic ring or hydrocarbon tail of surfactant. Models based on Flory-Huggins theory were employed to estimate CP from changes in mass distribution and to obtain cross-linking fractions. Surface tension measurements of non-irradiated and irradiated solutions were used for estimating the effectiveness and efficiency of surfactant in the formulation.

Theoretical and experimental analysis of ammonia ionic clusters produced by 252Cf fragment impact on an NH3 ice target.

Positive and negatively charged ammonia clusters produced by the impact of (252)Cf fission fragments (FF) on an NH(3) ice target have been examined theoretical and experimentally. The ammonia clusters generated by (252)Cf FF show an exponential dependence of the cluster population on its mass, and the desorption yields for the positive (NH(3))(n)NH(4)(+) clusters are 1 order of magnitude higher than those for the negative (NH(3))(n)NH(2)(-) clusters. The experimental population analysis of (NH(3))(n)NH(4)(+) (n = 0-18) and (NH(3))(n)NH(2)(-) (n = 0-8) cluster series show a special stability at n = 4 and 16 and n = 2, 4, and 6, respectively. DFT/B3LYP calculations of the (NH(3))(0)(-)(8)NH(4)(+) clusters show that the structures of the more stable conformers follow a clear pattern: each additional NH(3) group makes a new hydrogen bond with one of the hydrogen atoms of an NH(3) unit already bound to the NH(4)(+) core. For the (NH(3))(0)(-)(8)NH(2)(-) clusters, the DFT/B3LYP calculations show that, within the calculation error, the more stable conformers follow a clear pattern for n = 1-6: each additional NH(3) group makes a new hydrogen bond to the NH(2)(-) core. For n = 7 and 8, the additional NH(3) groups bind to other NH(3) groups, probably because of the saturation of the NH(2)(-) core. Similar results were obtained at the MP2 level of calculation. A stability analysis was performed using the commonly defined stability function E(n)(-)(1) + E(n)(+1) - 2E(n), where E is the total energy of the cluster, including the zero point correction energy (E = E(t) + ZPE). The trend on the relative stability of the clusters presents an excellent agreement with the distribution of experimental cluster abundances. Moreover, the stability analysis predicts that the (NH(3))(4)NH(4)(+) and the even negative clusters [(NH(3))(n)NH(2)(-), n = 2, 4, and 6] should be the most stable ones, in perfect agreement with the experimental results.

Ordered mesoporous silica SBA-15 A new effective adjuvant to induce antibody response

The applicability of SBA-15 mesostructure as an adjuvant and evaluation of its efficiency to induce antibody response, was discussed. It was observed that better encapsulation of biomolecules of variable shape and size can be achieved using a antigen to SBA-15 weight ratio of 1: 2.5. Efficient antibody generation could be achieved because SBA-15 was able to attract antigens effectively due to its high surface area and proper mesopore size. The results show that SBA-15 and related silica mesostructures are promising nanosystems for vaccine delivery.

Surto de varíola bovina causada pelo vírus Vaccinia na região da Zona da Mata Mineira / Outbreak of exantemal disease caused by Vaccinia virus in human and cattle in Zona da Mata region, Minas Gerais

Relata-se um surto de doença exantemática, caracterizada como varíola bovina, acometendo bovinos e seres humanos na Zona da Mata Mineira. Setenta e duas propriedades, distribuídas em 20 municípios localizados na região, foram visitadas para se levantar os aspectos clínicos e epidemiológicos da doença. Detectaram-se 1020 vacas doentes durante a investigação, quando houve queda na produção do leite associada a infecções bacterianas secundárias. Casos humanos foram registrados em 83 por cento das propriedades visitadas. Espécimes clínicos e amostras de soro foram coletados dos animais doentes ou convalescentes. O diagnóstico de laboratório mostrou o envolvimento de um ortopoxvírus, precisamente o Vaccinia virus como agente etiológico do surto.

Surto de varíola bovina causada pelo vírus Vaccinia na região da Zona da Mata Mineira / Outbreak of exantemal disease caused by Vaccinia virus in human and cattle in Zona da Mata region, Minas Gerais

Relata-se um surto de doença exantemática, caracterizada como varíola bovina, acometendo bovinos e seres humanos na Zona da Mata Mineira. Setenta e duas propriedades, distribuídas em 20 municípios localizados na região, foram visitadas para se levantar os aspectos clínicos e epidemiológicos da doença. Detectaram-se 1020 vacas doentes durante a investigação, quando houve queda na produção do leite associada a infecções bacterianas secundárias. Casos humanos foram registrados em 83% das propriedades visitadas. Espécimes clínicos e amostras de soro foram coletados dos animais doentes ou convalescentes. O diagnóstico de laboratório mostrou o envolvimento de um ortopoxvírus, precisamente o Vaccinia virus como agente etiológico do surto.(AU)

It was investigated an outbreak of exantemal disease in human and cattle in Zona da Mata region, Minas Gerais's State, Brazil. Seventy two farms located in 20 counties locating in this region were visited and disease pattern was studied. 1020 cows got sick in the visited herds and in 83% of them human cases occurred together with disease in animals. Drop in milk production and secondary infection were frequently observed. The disease occurred mainly from may to September. Serum and scars from sick and convalescent animals were collected and laboratory diagnostic showed that an orthopoxvirus, more precisely vaccinia virus was involved in the outbreak.(AU)

Laser induced formation of CsI ion clusters analyzed by delayed extraction time-of-flight mass spectrometry.

(CsI)nCs+ (n = 1,2) cluster ion formation from polycrystalline CsI irradiated by pulsed-UV laser (337 nm) is analyzed by delayed extraction time-of-flight mass spectrometry technique. Measurements were performed for different laser intensities and for several delayed extraction times. Experimental data show that CsI laser ablation produces the emission of (CsI),Cs+ ions (n = 0, 1, 2), whose yields decrease exponentially with n and increase exponentially with the laser pulse energy. A quasi equilibrium evolution of the clusters is proposed to extract a parameter characteristic of the cluster recombination process. The delayed extraction method of initial velocity determination was improved to take into account collisions in the high density plasma close to the target. The new parameterization helps to describe the dynamics of secondary ions of different masses for laser irradiances above the ion desorption threshold in a collision regime. The initial velocity of the secondary ions [(CsI)nCs+ (n = 0, 1, 2)] as function of the laser irradiance was determined. The distance to the target when the free expansion process starts is reported as function of the secondary ions mass and of the laser irradiance. The collision regime's influence on the secondary ion dynamics is discussed.