RESUMEN
OBJECTIVE: To design and fabricate canine rib prosthesis with full geometric shape using computed tomography (CT) scan combined with computer-aided design (CAD) and stereo lithographic (SLA) technologies and to evaluate the accuracy of this method. METHODS: After scanned on 64 rows helical CT, the cortex part of the right 7th rib was selected as the prototype for design and manufacture of the rib prosthesis and image data were stored as DICOM format. Three-dimensional (3D) surface reconstruction was applied to produce 3D image of the 7th rib and results were outputted as STL format which were then modified by UG software for establishment of CAD model. RESULTS: The rib prosthesis with full geometric shape was obtained based on CT scanning and SLA technique. About 30,000 point cloud data were acquired after 3D laser scan of the ribs. When comparing the rib prosthesis with the rib prototype, the maximum positive deviation, maximum negative deviation, average deviation and standard deviation were 1.764 mm, -2.126 mm, 0.183/-0.253 mm and 0.346 mm, respectively. There were about 88.17% of the point cloud data within the range of ±0.5 mm. CONCLUSION: It is feasible to design and fabricate rib prosthesis with full geometric shape by using CT scanning technology combined with CAD and SLA technologies. This method is fast, convenient and precise for manufacturing prosthesis. Optimization and improvement could be processed based on the deviation suggested by the scanning.
Asunto(s)
Diseño Asistido por Computadora , Prótesis e Implantes , Diseño de Prótesis/métodos , Costillas/diagnóstico por imagen , Tomografía Computarizada por Rayos X/métodos , Animales , Perros , Procesamiento de Imagen Asistido por Computador , Masculino , Tomografía Computarizada por Rayos X/instrumentaciónRESUMEN
Cu/Zn superoxide dismutase (SOD1) is a key antioxidant enzyme. Deficiency of SOD1 is associated with various human diseases, including cancer. Here, we report that SOD1 is succinylated and that succinylation decreases its activity. SIRT5 binds to, desuccinylates and activates SOD1. SOD1-mediated ROS reduction is increased when SIRT5 is co-expressed. Furthermore, mutation of the SOD1 succinylation site inhibits the growth of lung tumor cells. These results reveal a novel post-translational regulation of SOD1 by means of succinylation and SIRT5-dependent desuccinylation, which is important for the growth of lung tumor cells.
Asunto(s)
Especies Reactivas de Oxígeno/metabolismo , Sirtuinas/metabolismo , Succinatos/metabolismo , Superóxido Dismutasa/metabolismo , Secuencia de Aminoácidos , Biocatálisis , Línea Celular Tumoral , Proliferación Celular , Activación Enzimática , Humanos , Neoplasias Pulmonares/enzimología , Neoplasias Pulmonares/patología , Lisina/metabolismo , Datos de Secuencia Molecular , Mutación/genética , Unión Proteica , Superóxido Dismutasa/química , Superóxido Dismutasa-1RESUMEN
In this report, a full account of an extremely rare case on esophageal intramural dissection (EID) is presented. A 56-year-old female patient, misdiagnosed as esophageal mediastinal fistula under endoscopic view, was diagnosed correctly as EID with contained esophageal perforation in the operation and cured by thoracic esophagectomy.
