RESUMEN
Seven yeast strains, DMKU VGT1-14T, DMKU VGT1-19T, DMKU-JMGT1-28, DMKU-JMGT1-32, DMKU VGT2-06, DMKU VGT2-19 and DMKU VGT6-14, were isolated from a grease trap in Thailand and two strains, SJ-1 and SN-102 were isolated from the sea surface microlayer in Taiwan. On the basis of phenotypic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, these strains represented two novel yeast species of the genus Wickerhamiella. In terms of pairwise sequence similarity, four strains, DMKU VGT1-14, DMKU-JMGT1-32, DMKU VGT6-14 and SN-102, were closely related to Wickerhamiella infanticola NRRL Y-17858T but differed by 13 nucleotide substitutions with one gap (2.46â%) in the D1/D2 domain of the LSU rRNA gene and 15 nucleotide substitutions with 23 gaps (4.2â%) in the ITS region. The strains DMKU VGT1-19T, DMKU-JMGT1-28, DMKU VGT2-06, DMKU VGT2-19 and SJ-1, differed from the type strain of the most closely related species, Wickerhamiella sorbophila NRRL Y-7921T, by nine nucleotide substitutions with one gap (1.66â%) in the D1/D2 domain of the LSU rRNA gene and nine nucleotide substitutions with 17 gaps (2.52%) in the ITS region. Hence, the names Wickerhamiella osmotolerans sp. nov. and Wickerhamiella tropicalis sp. nov. are proposed to accommodate these species in the genus Wickerhamiella. The holotypes are W. osmotolerans DMKU VGT1-14T (ex-type strain TBRC 11425=PYCC 8359=CGMCC 2.6179; Mycobank number 833394) and W. tropicalis DMKU VGT1-19T (ex-type strain TBRC 11426=PYCC 8360=CGMCC 2.6180; Mycobank number 833393).
Asunto(s)
Filogenia , Saccharomycetales/clasificación , Agua de Mar/microbiología , ADN de Hongos/genética , ADN Espaciador Ribosómico/genética , Técnicas de Tipificación Micológica , Saccharomycetales/aislamiento & purificación , Análisis de Secuencia de ADN , Taiwán , TailandiaRESUMEN
In the present study, we found that our isolate Shewanella decolorationis NTOU1 is able to degrade acetate under anaerobic condition with concomitant implementation of extracellular electron transfer (EET). With +0.63 V (vs. SHE) poised on the anode, in a 72-h experiment digesting acetate, only 2 mM acetate was consumed, which provides 6% of the electron equivalents derived from the initial substrate mass to support biomass (5%) and current generation (1%). To clarify the effects on EET of the addition of electron-shuttles, riboflavin, anthraquinone-2,6-disulfonate (AQDS), hexaammineruthenium, and hexacyanoferrate were selected to be spiked into the electrochemical cell in four individual experiments. It was found that the mediators with proton-associated characteristics (i.e., riboflavin and AQDS) would not enhance current generation, but the metal-complex mediators (i.e., hexaammineruthenium, and hexacyanoferrate) significantly enhanced current generation as the concentration increased. According to the results of electrochemical analyses, the i-V graphs represent that the catalytic current induced by the primitive electron shuttles started at the onset potential of -0.27 V and continued increasing until +0.73 V. In the riboflavin-addition experiment, the catalytic current initiated at the same potential but rapid saturated beyond -0.07 V; this indicated that the addition of riboflavin affects mediator secretion by S. decolorationis NTOU1. It was also found that the current was eliminated after adding 48 mM N-acetyl-L-methionine (i.e., the cytochrome inhibitor) when using acetate as a substrate, indicating the importance of outer-membrane cytochrome.
RESUMEN
Cystobasidium keelungensis SN2T (CBS 6949 = BCRC 920080), a new anamorphic basidiomycetous yeast species, is described in this paper. The strains belonging to this species were isolated from the sea surface microlayer and underlying water in Taiwan. These strains were identified by examining nucleotide sequences in the species-specific D1/D2 domains of the large subunit (LSU) ribosomal RNA (rRNA) and by evaluating their physiological characteristics. Phylogenetic analyses of D1/D2 sequences revealed that C. keelungensis was most closely related to the species C. slooffiae (LSU rRNA gene sequence divergence of 1.5%), and it belonged to the Erythrobasidium clade. No sexual reproduction was observed. This species differed from related species in carbon and nitrogen assimilation patterns and growth at 35 °C. Screening C. keelungensis for the presence of UV-absorbing compounds showed that mycosporine-glutaminol-glucoside and mycosporine-glutamicol-glucoside (maximum absorption: 310 nm) were the major UV-absorbing compounds, which differ from the compounds present in some freshwater yeast strains reported in previous studies. After UV induction, SN2 had a higher level of mycosporine production than other carotenogenic yeasts in this study.
Asunto(s)
Basidiomycota , Agua de Mar/microbiología , Secuencia de Bases , Basidiomycota/clasificación , Basidiomycota/genética , Basidiomycota/aislamiento & purificación , Ciclohexanoles/metabolismo , ADN de Hongos/genética , ADN Espaciador Ribosómico/genética , Glucósidos/metabolismo , Técnicas de Tipificación Micológica , Océanos y Mares , Filogenia , ARN Ribosómico/genética , Análisis de Secuencia de ADN , TaiwánRESUMEN
Using an electrochemical cell equipped with carbon felt electrodes (poised at +0.63â¯V vs. SHE), the current production capabilities of two Shewanella strains-NTOU1 and KR-12-were examined under various conditions with lactate as an electron donor. The metabolic charge produced in the tricarboxylic acid cycle (QTCA) was calculated by mass-balance. The data showed a linear relation between the electric coulomb production (QEL) and QTCA with an R2 of 0.65. In addition, a large amount of pyruvate accumulation was observed at pHâ¯=â¯6, rendering QTCA negative. The results indicate an occurrence of an undesired cataplerotic reaction. It was also found that QTCA provides important information showing the oxygen-boosting TCA cycle and anodic-current generation of Shewanella spp. Linear dependence of the change in charge for biomass growth (4.52FΔnCell) on QTCA was also found as expressed by 4.52FΔnCellâ¯=â¯1.0428 QTCAâ¯+â¯0.0442, indicating that these two charge quantities are inherently identical under most of the experimental conditions. In the mediator-spiked experiments, the external addition of the mediators (ferricyanide, anthraquinone-2, 6-disulfonate, and riboflavin) beyond certain concentrations inhibited the activity of the TCA cycle, indicating that the oxidative phosphorylation is deactivated by excessive amounts of mediators, yet Shewanella spp. are constrained with regard to carrying out the substrate-level phosphorylation.
Asunto(s)
Ciclo del Ácido Cítrico , Shewanella/metabolismo , Antraquinonas/química , Biomasa , Transporte de Electrón , Ferricianuros/química , Concentración de Iones de Hidrógeno , Ácido Láctico/metabolismo , NAD/metabolismo , Oxidación-Reducción , Fosforilación Oxidativa , Riboflavina/química , Shewanella/crecimiento & desarrollo , Ácidos Sulfónicos/químicaRESUMEN
Yeast communities inhabiting the sea surface microlayer (SSML) on the northern coast of Taiwan were examined using a cultivation method and compared with those inhabiting the underlying water (UW) at a 50-cm depth. Culturable yeasts were recovered from the SSML and UW samples collected in the morning during 4 field campaigns, and 420 strains were isolated. The 420 isolates were grouped into 43 species using a polyphasic molecular approach, including sequence analysis of the 26S rDNA D1/D2 domain and 5.8S-ITS region. From the SSML samples, 12 genera and 39 species, including 7 new species of Cryptococcus sp. (1), Candida spp. (4), and Rhodotorula spp. (2), were isolated. From the UW samples, 10 genera and 21 species, including one new species of Rhodotorula sp. (1), were isolated. Rhodotorula mucilaginosa was the most abundant species present in the yeast community in SSML (37.6%) and UW (21.6%) samples. Basidiomycetous yeasts (63.6%) and pigmented yeasts (64.5%) comprised the major yeast population. The yeast community in the SSML had a higher species number and abundance than the UW. Moreover, although the majority of yeast community species were from the SSML, individual species distribution in the SSML was unequal.
Asunto(s)
Biodiversidad , Océanos y Mares , Agua de Mar/microbiología , Levaduras/clasificación , Levaduras/aislamiento & purificación , Basidiomycota/genética , Basidiomycota/aislamiento & purificación , Candida/genética , Candida/aislamiento & purificación , ADN de Hongos/genética , ADN Ribosómico/genética , Filogenia , Reacción en Cadena de la Polimerasa , Rhodotorula/genética , Rhodotorula/aislamiento & purificación , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/aislamiento & purificación , Análisis de Secuencia de ADN , Taiwán , Levaduras/genéticaRESUMEN
Ascomycetous yeast strain SM-22 was isolated from the sea-surface microlayer near the Keelung City off the northern coast of Taiwan. This strain showed a cell surface hydrophobicity higher than 90 %, moderate UV A/B resistance, and it degraded 68 % of the total petroleum hydrocarbon content of an artificial seawater medium containing 1 % (v v(-1)) diesel oil within 15 days at 25 °C. The closest phylogenetic relative of this strain is Candida oslonensis CBS 10146(T), but it differs from strain SM-22 by a 3.7 % divergence (including 18 nucleotide substitutions and 2 gaps) in the D1/D2 domain sequence of the large subunit rRNA gene. This difference clearly suggests that the strain SM-22 represents a distinct species. Strain SM-22 does not produce ascospores on common sporulation media and it can therefore be considered an anamorph of the genus Yarrowia. Thus, the name Yarrowia keelungensis sp. nov. (type strain SM-22(T) = BCRC 23110(T) = JCM 14894(T) = CBS 11062(T)) is proposed as a novel species of genus Yarrowia.
Asunto(s)
Aceites/metabolismo , Agua de Mar/microbiología , Yarrowia/clasificación , Yarrowia/aislamiento & purificación , Biotransformación , Análisis por Conglomerados , Medios de Cultivo/química , ADN de Hongos/química , ADN de Hongos/genética , ADN Intergénico/química , ADN Intergénico/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Genes de ARNr , Microscopía , Datos de Secuencia Molecular , Técnicas de Tipificación Micológica , Filogenia , ARN de Hongos/genética , ARN Ribosómico/genética , Análisis de Secuencia de ADN , Esporas Fúngicas/crecimiento & desarrollo , Taiwán , Yarrowia/crecimiento & desarrollo , Yarrowia/metabolismoRESUMEN
This is the first study to examine the application parameters for oxidation of two sulfonamide antibiotics (SAs), sulfadimethoxine (SDM) and sulfamonomethoxine (SMM), using a novel laccase and mediators. The optimal conditions in the laccase-mediator system (LMS) were pH 4, 50-60 °C, and 1 mM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS); pH 4, 40-60 °C, and 1 mM for violuric acid (VLA); pH 6, 50 °C, and 2 mM for syringaldehyde (SIR). Additionally, the conditions of the 4-hydroxybenzyl alcohol (HBA) mediator were pH 4, 30 °C, and 2 mM in the oxidation of SDM; and the temperature increased to 60 °C for SMM. The laccase coupled with VLA and HBA resulted in the lowest toxicity of the SA solutions during processing, whereas treatments with ABTS and SIR resulted in higher toxicities. Furthermore, the laccase used in this study was stable and resistant to dialysis, thus can be reused for oxidation process.
Asunto(s)
Antibacterianos/química , Lacasa/química , Sulfonamidas/química , Purificación del Agua/métodos , Antibacterianos/análisis , Biodegradación Ambiental , Concentración de Iones de Hidrógeno , Oxidación-Reducción , Sulfonamidas/análisis , TemperaturaRESUMEN
The enzymatically active region of amylopullulanase from Thermoanaerobacterium saccharolyticum NTOU1 (TsaNTOU1Apu) was identified by truncation mutagenesis. Two truncated TsaNTOU1Apu enzymes, TsaNTOU1ApuM957 and TsaNTOU1ApuK885, were selected and characterized. Both TsaNTOU1ApuM957 and TsaNTOU1ApuK885 showed similar specific activities toward various substrates. The overall catalytic efficiency (k (cat)/apparent K (m)) for the soluble starch or pullulan substrate, however, was 20-25% lower in TsaNTOU1ApuK885 than in TsaNTOU1ApuM957. Both truncated enzymes exhibited similar thermostability and substrate-binding ability against the raw starch. The fluorescence and circular dichroism spectrometry studies indicated that TsaNTOU1ApuK885 retained an active folding conformation similar to that of TsaNTOU1ApuM957. These results indicate that a large part of the TsaNTOU1Apu, such as the C-terminal carbohydrate-binding module family 20, the second fibronectin type III, and a portion of the first FnIII motifs, could be removed without causing a serious aberrant structural change or a dramatic decrease in hydrolysis of soluble starch and pullulan.
Asunto(s)
Proteínas Bacterianas/metabolismo , Escherichia coli/enzimología , Glucanos/metabolismo , Glicósido Hidrolasas/metabolismo , Isoenzimas/metabolismo , Proteínas Recombinantes/metabolismo , Thermoanaerobacterium/enzimología , Proteínas Bacterianas/genética , Dominio Catalítico , Dicroismo Circular , Clonación Molecular , Estabilidad de Enzimas , Escherichia coli/genética , Glicósido Hidrolasas/genética , Calor , Hidrólisis , Isoenzimas/genética , Cinética , Plásmidos , Unión Proteica , Estructura Terciaria de Proteína , Proteínas Recombinantes/genética , Almidón/metabolismo , Especificidad por Sustrato , Thermoanaerobacterium/genética , Transformación BacterianaRESUMEN
A new extremely thermophilic, anaerobic, gram-negative bacterium, strain NTOU1, was enriched and isolated from acidic marine hydrothermal fluids off Gueishandao island in Taiwan with 0.5% starch and 0.5% maltose as carbon sources. This strain was capable of growth utilizing various sugars found in lignocellulosic biomass as well as xylan and cellulose, and produced ethanol, lactate, acetate, and CO(2) as fermentation products. The results of a 16S rRNA gene sequence analysis (1,520 bp) revealed NTOU1 to belong to the genus Thermoanaerobacterium. When tested for the ability to grow and produce ethanol from xylose or rice straw hemicellulosic hydrolysate at 70°C, the strain showed the highest levels of ethanol production (1.65 mol ethanol mol xylose(-1)) in a medium containing 0.5% xylose plus 0.5% yeast extract. Maximum ethanol production from the rice straw hemicellulose was 0.509 g g(-1), equivalent to 98.8% theoretical conversion efficiency. Low concentrations of inhibitors (derived from dilute acid hydrolysis) in the rice straw hemicellulose hydrolysate did not affect the ethanol yield. Thus, Thermoanaerobacterium strain NTOU1 has the potential to be used for ethanol production from hemicellulose.
Asunto(s)
Etanol/metabolismo , Respiraderos Hidrotermales/microbiología , Thermoanaerobacterium/aislamiento & purificación , Thermoanaerobacterium/metabolismo , Anaerobiosis , Metabolismo de los Hidratos de Carbono , Análisis por Conglomerados , Medios de Cultivo/química , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Calor , Datos de Secuencia Molecular , Oryza/metabolismo , Oryza/microbiología , Filogenia , Tallos de la Planta/metabolismo , Tallos de la Planta/microbiología , Polisacáridos/metabolismo , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Taiwán , Thermoanaerobacterium/genética , Thermoanaerobacterium/fisiologíaRESUMEN
Glutaredoxins (Grxs) play important roles in the redox system via reduced glutathione as a reductant. A TcmonoGrx cDNA (1039 bp, EU158772) encoding a putative monothiol Grx was cloned from Taiwanofungus camphorata (formerly named Antrodia camphorata). The deduced amino acid sequence is conserved among the reported monothiol Grxs. Two 3-D homology structures of the TcmonoGrx based on known structures of human Grx3 (pdb: 2DIY_A) and Mus musculus Grx3 (pdb: 1WIK_A) have been created. To characterize the TcmonoGrx protein, the coding region was subcloned into an expression vector pET-20b(+) and transformed into E. coli C41(DE3). The recombinant His6-tagged TcmonoGrx was overexpressed and purified by Ni(2+)-nitrilotriacetic acid Sepharose. The purified enzyme showed a predominant band on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme exhibited glutathione reductase (GR) activity via dithionitrobenzoate (DTNB) assay. The Michaelis constant (K(M)) values for GSSG and NADPH were 0.064 and 0.041 mM, respectively. The enzyme's half-life of deactivation at 60 °C was 10.5 min, and its thermal inactivation rate constant (k(d)) was 5.37 × 10(-2) min(-1). The enzyme was active under a broad pH range from 6 to 8. The enzyme retained 50% activity after trypsin digestion at 37 °C for 40 min. Both mutants C(40)âS(40) and C(165)âS(165) lost 40-50% GR activity, whereas the mutant S(168)âC(168) showed a 20% increase in its GR activity.
Asunto(s)
Agaricales/enzimología , Glutarredoxinas/genética , Glutatión Reductasa/metabolismo , Agaricales/genética , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , Cartilla de ADN , Electroforesis en Gel de Poliacrilamida , Glutarredoxinas/química , Glutarredoxinas/metabolismo , Glutatión Reductasa/química , Semivida , Humanos , Cinética , Modelos Moleculares , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Homología de Secuencia de AminoácidoRESUMEN
A xylanase gene was PCR-cloned from Thermoanaerobacterium saccharolyticum and expressed in Escherichia coli. The xylanase (XynA) consisted of a signal peptide, glycoside hydrolase family 10 domains, carbohydrate-binding modules, and surface layer homology domains. It was optimally active at 70-73°C and at pH 5-7. It had enhanced activity with NaCl with optimal activity at 0.4 M but was tolerant up to 2 M NaCl. The thermostable and salt-tolerant properties of this xylanase suggest that it may be useful for industrial applications.
Asunto(s)
Sales (Química)/metabolismo , Thermoanaerobacterium/enzimología , Xilosidasas/genética , Xilosidasas/metabolismo , Clonación Molecular , ADN Bacteriano/química , ADN Bacteriano/genética , Activadores de Enzimas/metabolismo , Estabilidad de Enzimas , Escherichia coli/genética , Expresión Génica , Concentración de Iones de Hidrógeno , Datos de Secuencia Molecular , Señales de Clasificación de Proteína/genética , Estructura Terciaria de Proteína , Análisis de Secuencia de ADN , Temperatura , Xilosidasas/químicaRESUMEN
The effect of concentration of coplanar PCB on the dechlorinating microbial community and dechlorination were investigated in anoxic estuarine sediment collected from Er-Jen River and enriched with 10 and 50 mg L⻹ of 3,4,4',5-tetrachlorobiphenyl, 3,3',4,4',5-pentachlorobiphenyl, and 3,3',4,4',5,5'-hexachlorobipheny. Dechlorination rates were similar in the cultures enriched with 10 and 50 mg L⻹ of 3,4,4',5-tetrachlorobiphenyl, whereas significantly higher dechlorination rates were observed in cultures enriched with 10 mg L⻹ of 3,3',4,4',5-pentachlorobiphenyl. No dechlorination was observed in sediment slurries enriched with 3,3',4,4',5,5'-hexachlorobipheny. Para dechlorination occurred prior to meta dechlorination during reductive dechlorination of 3,4,4',5-tetrachlorobiphenyl and 3,3',4,4',5-pentachlorobiphenyl. GC-MS and denaturing gradient gel electrophoresis (DGGE) were used to detect dechlorination products and dechlorinating microorganisms in the enriched sediment cultures during the process of degradation. Two Chloroflexi phylotypes observed in DGGE were responsible for para and meta dechlorination respectively. Phylotype Cp-1 has 98% similarity to uncultured bacterium N5-12. Phylotype Cm-1 has 99% similarity to uncultured dechlorinating bacterium m1 or SF1 belonging to the ο-17/DF-1 group of PCB-dechlorinating bacteria.
Asunto(s)
Agua Dulce/química , Sedimentos Geológicos/química , Bifenilos Policlorados/análisis , Contaminantes Químicos del Agua/análisis , Secuencia de Bases , Biota , Chloroflexi/efectos de los fármacos , Chloroflexi/genética , Chloroflexi/crecimiento & desarrollo , Agua Dulce/microbiología , Sedimentos Geológicos/microbiología , Halogenación/efectos de los fármacos , Datos de Secuencia Molecular , Bifenilos Policlorados/toxicidad , Contaminantes Químicos del Agua/toxicidadRESUMEN
L-rhamnose isomerase (EC 5.3.1.14, L-RhI) catalyzes the reversible aldose-ketose isomerization between L-rhamnose and L-rhamnulose. In this study, the L-Rhi gene encoding L-Rhi was PCR-cloned from Thermoanaerobacterium saccharolyticum NTOU1 and then expressed in Escherichia coli. A high yield of the active L-RhI, 9780 U/g of wet cells, was obtained in the presence of 0.2 mM IPTG induction. L-RhI was purified sequentially using heat treatment, nucleic acid precipitation, and anion-exchange chromatography. The purified L-RhI showed an apparent optimal pH of 7 and an optimal temperature at 75 °C. The enzyme was stable at pH values ranging from 5 to 9, and the activity was fully retained after a 2 h incubation at 40-70 °C. L-RhI from T. saccharolyticum NTOU1 is the most thermostable L-RhI to date, and it has a high specific activity (163 U/mg) and an acceptable purity after heat treatment, suggesting that this enzyme has the potential to be used in rare sugar production.
Asunto(s)
Isomerasas Aldosa-Cetosa/genética , Thermoanaerobacterium/enzimología , Isomerasas Aldosa-Cetosa/química , Isomerasas Aldosa-Cetosa/metabolismo , Secuencia de Aminoácidos , Clonación Molecular , Estabilidad de Enzimas , Escherichia coli/genética , Fructosa/biosíntesis , Expresión Génica , Glucosa/biosíntesis , Calor , Concentración de Iones de Hidrógeno , Datos de Secuencia Molecular , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Thermoanaerobacterium/genéticaRESUMEN
The toxicity of three coplanar PCBs on microbial communities of an estuarine sediment were assessed. Sediment slurries were amended with 2, 10 and 50 mg/L of 345-4 CB, 345-34 CB and 345-345 CB, respectively under anaerobic conditions. The fate and effects of these coplanar PCBs were studied over 250 days. Bacterial communities in sediment slurries were described by dehrdogenase activity and by bacterial populations deduced from the clone libraries. Dechlorination of 345-4 CB and 345-34 CB occurred at least after 100 days of incubation, but dechlorination of 345-345 CB was not observed over the entire incubation period. However, time profiles of dehydrogenase activity were similar in sediment slurries amended with 345-4 CB, 345-34 CB or 345-345 CB. After normalization of the effect of acetone we found that dehydrogenase activity was increased in sediment slurries amended with 50 mg/L, but were inhibited in those amended with 2 and 10 mg/L of coplanar PCBs. Extra addition of electron donors plus sulfate or sulfate could increase dehydrogenase activity significantly. The major microbial populations in the sediment slurries incubated with 2, 10, and 50 mg/L of 345-4 CB were delta-Proteobacteria, Chloroflexi, and epsilon-Proteobacteria, respectively. This study shows that (1) dechlorination of coplanar PCBs did not change their effects on microbial metabolic activities, (2) concentration of coplanar PCBs had effects on microbial metabolic activities and community composition, (3) extra addition of electron donors plus sulfate or sulfate could increase dehydrogenase activity significantly, but this did not always lead to higher dechlorination rates, (4) coplanar PCBs induced perturbations of sediment microbial communities in terms of population structures (but not always as an inhibition).
Asunto(s)
Contaminantes Ambientales/toxicidad , Sedimentos Geológicos/microbiología , Bifenilos Policlorados/química , Bifenilos Policlorados/toxicidad , Microbiología del Suelo , Microbiología del Agua , Anaerobiosis/efectos de los fármacos , Bacterias/clasificación , Bacterias/efectos de los fármacos , Bacterias/genética , Bacterias/metabolismo , Cloro/química , ADN Bacteriano/genética , ADN Bacteriano/metabolismo , Contaminantes Ambientales/química , Contaminantes Ambientales/metabolismo , Agua Dulce , Biblioteca de Genes , Sedimentos Geológicos/química , Oxidorreductasas/metabolismo , Filogenia , Bifenilos Policlorados/metabolismo , Agua de Mar , Factores de TiempoRESUMEN
Enrofloxacin (ENR) and ciprofloxacin (CIP) are two fluoroquinolone (FQ) antibiotics widely used to treat diseases of human beings and cultured animals. These two FQs are usually detected in the effluent of municipal sewage plants and related aquatic environments. The purpose of this study was to understand the fates of ENR and CIP in aquaculture pond water and a sediment slurry in a laboratory-scale experiment. Effects of light and microbial activity on the degradation of these two FQs were investigated. Results indicated that natural irradiation plays a major role in the degradation of ENR and CIP in pond water and the sediment slurry. The 50 % dissipation times (DT(50)) with non-sterile treatment were 0.01 and 18.4 d for ENR, and 0.04 and 17.3 d for CIP in the water and sediment slurry, respectively. On the other hand, the degradation of ENR and CIP under dark conditions was slow or even hindered, and all of their DT(50) values exceeded 100 d. These two FQs degraded faster in the sediment slurry than in pond water under dark conditions. Artificial ultraviolet (UV) and fluorescence light had similar effects on the degradation of ENR in the pond water and sediment slurry. Degradation of CIP was faster with UV than with fluorescence light treatment, while no such difference was found for ENR degradation. CIP was a degradation product of ENR under both light and dark conditions, and DT(50) values for both compounds were shorter in the presence of light. The phenomenon of biodegradation was observed during degradation of CIP in the sediment slurry under natural light.
Asunto(s)
Antibacterianos/efectos de la radiación , Fluoroquinolonas/efectos de la radiación , Agua Dulce/química , Sedimentos Geológicos/química , Contaminantes del Suelo/efectos de la radiación , Luz Solar , Contaminantes Químicos del Agua/efectos de la radiación , Antibacterianos/metabolismo , Acuicultura , Biodegradación Ambiental , Fluoroquinolonas/metabolismo , Agua Dulce/microbiología , Sedimentos Geológicos/microbiología , Contaminantes del Suelo/química , Contaminantes del Suelo/metabolismo , Rayos Ultravioleta , Contaminantes Químicos del Agua/metabolismoRESUMEN
To clarify the major factor caused by oxygen-enhancing charge production of Shewanella decolorationis NTOU1 towards a polarized anode, a series of experimental runs (i.e., with/without ambient air flushing and with/without ammonia addition as nitrogen source) were conducted in this study. Within 6-day of operation at +0.4 V vs. Ag|AgCl and starting with 35 mM of lactate, consistently the electrical charge production under the aerobic condition was higher than that under the anaerobic condition. In all the experimental runs, the values of nicotinamide adenine dinucleotide (NADH) production were found to be correlated positively and significantly with the charge production, but the highest Coulombic efficiency of 18% was observed under the anaerobic conditions without ammonia addition while the lowest charge production occurred. Those results indicate that NADH production enhanced by oxygen is the leading cause of the increase of the charge production, but the biomass production and the oxygen reduction would both consume NADH electrons and lead to lower electron recoveries. In addition, whether under constant aerobic or anaerobic, or alternating aerobic/anaerobic conditions, chronoamperometric results made it possible to rule out other factors, like lactate uptake rate or cell growth, which might increase the charge production under aerobic conditions. By using high performance liquid chromatography, some diffusive flavins (e.g., 0.5 microM of riboflavin) were found under the aerobic condition, but were not found under the anaerobic one. However, from results of cyclic voltammetry (CV), the signals of flavins were found to be approximately the same under both conditions. Although it is inferred that oxygen renders the flavins secreted extracellularly, that is not the major effect of oxygen for boosting the charge production. Furthermore, bound flavins under anaerobic condition were found to be effectively electrocatalytic according to sigmoidal CV result.
Asunto(s)
Fuentes de Energía Bioeléctrica/microbiología , Técnicas Biosensibles , Transporte de Electrón , Oxígeno/metabolismo , Shewanella/metabolismo , Aerobiosis , Amoníaco/metabolismo , Anaerobiosis , Cromatografía Líquida de Alta Presión , Técnicas Electroquímicas , Ácido Láctico/metabolismo , NAD/biosíntesis , Oxidación-ReducciónRESUMEN
The possible causative agent and shrimp species involved in a bait shrimp poisoning case that occurred in northern Taiwan was determined. Because the patient's symptoms were similar to those caused by boric acid and slightly similar to those caused by sulfite, the concentrations of boric acid and sulfite (as sulfur dioxide) in the patient's vomitus and in shrimp collected from bait stores and markets were analyzed. The concentration of boric acid was 36.8 to 37.1 mg/g in the patient's vomitus, 1.4 to 3.8 mg/g in shrimp meats obtained from bait stores, and not detectable (less than 0.001 mg/g) in shrimp meat obtained from commercial markets. No significant differences in sulfur dioxide concentrations (0.067 to 0.088 mg/g) were found in patient's vomitus and the shrimp meat from both bait stores and commercial markets. A fragment of the cytochrome b gene (â¼406 bp) was amplified by PCR using a pair of primers (UCYTB151F and UCYTB270R) from shrimp meat of two species and the vomitus. The vomited shrimp was identified as Parapenaeus fissuroides on the basis of gene sequencing and restriction fragment length polymorphism patterns after treatment with endonuclease Alu I. Based on the patient's symptoms and analytical data, we concluded that boric acid at toxic levels had been illegally added to the bait shrimp P. fissuroides.
Asunto(s)
Ácidos Bóricos/análisis , Contaminación de Alimentos/análisis , Penaeidae/química , Mariscos/análisis , Dióxido de Azufre/análisis , Animales , Ácidos Bóricos/toxicidad , Seguridad de Productos para el Consumidor , Grupo Citocromo b/genética , Microbiología de Alimentos , Humanos , Penaeidae/clasificación , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Intoxicación por Mariscos/diagnóstico , Especificidad de la Especie , Dióxido de Azufre/toxicidad , Taiwán , VómitosRESUMEN
A new ascomycetous yeast species, Candida neustonensis is proposed in this study based on four strains (SN92(T), SN47, SJ22, SJ25) isolated from sea surface microlayer in Taiwan. These four yeast strains were morphologically, physiologically and phylogenetically identical to each other. No sexual reproduction was observed on 5% malt extract agar, corn meal agar, V8 agar, McClary's acetate agar and potato-dextrose agar. Phylogenetic analysis of the sequences of the D1/D2 domain of the large subunit (LSU) rRNA gene places C. neustonensis as a member of the Pichia guilliermondii clade, it also reveals that the phylogenetically closest relatives of C. neustonensis are C. fukuyamaensis (4.4% divergence), C. xestobii (4.4% divergence) and P. guilliermondii (4.5% divergence). C. neustonensis also is clearly distinguished from other known species in the P. guilliermondii clade based on the results of physiology tests. From these comparison analyses, the following novel yeast species is proposed: Candida neustonensis sp. nov., with strain SN92(T) (= BCRC 23108(T) = JCM 14892(T) = CBS 11061(T)) as the type strain.
Asunto(s)
Candida/clasificación , Candida/aislamiento & purificación , Agua de Mar/microbiología , Candida/genética , Candida/fisiología , Análisis por Conglomerados , ADN de Hongos/química , ADN de Hongos/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Genes de ARNr , Datos de Secuencia Molecular , Técnicas de Tipificación Micológica , Filogenia , ARN de Hongos/genética , ARN Ribosómico/genética , Recombinación Genética , Análisis de Secuencia de ADN , TaiwánRESUMEN
Shewanella sp. NTOU1 was able to decolorize a range of anthraquinone dyes [Reactive Blue 4 (RB4), Reactive Blue 19 (RB19), Mordant Red 11 (MR11), Disperse Red 15 (DR15), and Disperse Blue 3 (DB3)] under anaerobic conditions. By supplementing the medium with formate and ferric citrate as the electron donor and acceptor, respectively and cultivating it under the optimum pH (8-9) and temperature (45 degrees C), this strain could decolorize these dyes (1,000 mg/L) at the initial color removal rates of 15-126 mg/L/h and the rates among them were RB19 > RB4 > DB3 > DR15 > MR11. The extent of color removal was in the range of 90-98% for RB19, 86-96% for RB4, 39-41% for MR11, 69-82% for DR15, and 89-91% for DB3. Based on the decolorization products detected by means of GC/MS analyses, probable pathways for the decolorization of these dyes by this strain were proposed.