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This study investigated non-thermal pretreatment (cold plasma, CP) on the flavor (taste and odor) profiles of dried fish products. CP treatment of 5 min contributed to accumulation of umami nucleotides adenosine 5'-monophosphate (AMP) from 30.96 to 40.82 µg/g and inosine 5'-monophosphate (IMP) from 2009.29 to 2132.23 µg/g, and significant reduction of bitter hypoxanthine ribonucleoside (HxR) and hypoxanthine (Hx), respectively (P < 0.05) in the dried fish products. A noticeable enhancement in sweet glycine (from 429.41 to 490.03 mg/100 g) and umami glutamic acid (from 55.68 to 67.76 mg/100 g) accompanied with the CP treatment (P < 0.05) based on taste activity value (TAV > 1). And the characteristic odor volatiles (nonanal, hexanal and 1-octen-3-ol) were strengthened 2.13-, 2.16- and 2.17- folds, respectively (P < 0.05). The results of equivalent umami concentration and Gibbs free energy calculation, combining with the correlation analysis, indicate that nucleotides and free amino acids synergically enhanced the taste improvement of dried fish products. Moderate lipids oxidation favored the formation of characteristic volatiles. The CP pretreatment offered new strategies for enhancing flavor of dried fish products.
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Carpas , Productos Pesqueros , Aromatizantes , Odorantes , Gusto , Animales , Odorantes/análisis , Productos Pesqueros/análisis , Carpas/metabolismo , Aromatizantes/química , Gases em Plasma/química , Humanos , Compuestos Orgánicos Volátiles/química , Conservación de Alimentos/métodosRESUMEN
The aftertaste with a prolonged duration in ampelopsis grossedentata infusion (AGTI) is easily perceived, however, its formation mechanism is unclear. Therefore, aftertaste-A and richness were confirmed as the characteristic aftertaste of AGTI through sensory evaluation and electronic tongue. Moreover, 5-KETE, theobromine, etc., metabolites were identified as the differential components between AGTI and green tea infusion. Among them, p-coumaroyl quinic acid, xanthine etc., and proline, dihydromyricetin, etc., components contributed more to the formation of aftertaste-A and richness, respectively. Further, the bonding between characteristic metabolites for aftertaste in AGTI with their receptors were shown to be more stable using molecular docking, compared to metabolites related to typical taste profiles. The aftertaste in AGTI was more easily perceived by saltiness components or in NaCl system by molecular simulation. This study offers novel insight into the interaction mechanism of aftertaste in tea infusion and will contribute to further study on aftertaste for other foods.
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Ampelopsis , Gusto , Humanos , Ampelopsis/química , Ampelopsis/metabolismo , Metabolómica , Simulación del Acoplamiento Molecular , Masculino , Femenino , Adulto , Aromatizantes/química , Aromatizantes/metabolismo , Adulto Joven , Té/química , Té/metabolismo , Extractos Vegetales/química , Extractos Vegetales/metabolismoRESUMEN
BACKGROUND: This study aimed to assess the diagnostic potential of serum intestinal fatty acid-binding protein (I-FABP), fecal calprotectin (FC), and fecal human ß-defensin 2 (hBD2) in predicting necrotizing enterocolitis (NEC) in preterm infants. METHODS: A prospective cohort of neonates with a gestational age < 32 weeks, suspected of NEC, was enrolled between June 2021 and December 2022. Serum I-FABP, FC, and fecal hBD2 levels were measured upon NEC suspicion, and diagnosis was confirmed through radiological examination or surgical intervention. Diagnostic precision of serum I-FABP, FC, and fecal hBD2 was assessed using a logistic regression model with multiple variables. RESULTS: The study included 70 neonates (45 males, 25 females), with 30 developing NEC (40% Stage III, n = 12; 60% Stage II, n = 18) and 40 in the control group. NEC patients exhibited significantly higher serum I-FABP and FC levels (4.76 ng/mL and 521.56 µg/g feces, respectively) than those with other diagnoses (1.38 ng/mL and 213.34 µg/g feces, respectively; p Ë 0.05 for both biomarkers). Stage II NEC neonates showed elevated fecal hBD2 levels (376.44 ng/g feces) than Stage III NEC neonates and controls (336.87 ng/g and 339.86 ng/g feces, respectively; p Ë 0.05). No such increase was observed in infants progressing to Stage III NEC. Using a serum I-FABP threshold of > 2.54 ng/mL yielded 76.7% sensitivity, 87.5% specificity, 82.1% positive predictive value (PPV), and 83.3% negative predictive value (NPV). For FC (cutoff > 428.99 µg/g feces), corresponding values were 76.7% sensitivity, 67.5% specificity, 63.9% PPV, and 79.4% NPV. CONCLUSION: Serum I-FABP and FC levels are valuable for early NEC detection and provide insights into disease severity. Low fecal hBD2 levels suggest an inadequate response to luminal bacteria, potentially rendering these infants more susceptible to NEC development or exacerbation.
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Enterocolitis Necrotizante , Enfermedades del Recién Nacido , beta-Defensinas , Masculino , Lactante , Femenino , Recién Nacido , Humanos , Recien Nacido Prematuro , Enterocolitis Necrotizante/metabolismo , Complejo de Antígeno L1 de Leucocito/metabolismo , beta-Defensinas/metabolismo , Estudios Prospectivos , Proteínas de Unión a Ácidos Grasos , Heces , Biomarcadores/metabolismoRESUMEN
BACKGROUND: The U-box gene family encodes E3 ubiquitin ligases involved in plant hormone signaling pathways and abiotic stress responses. However, there has yet to be a comprehensive analysis of the U-box gene family in maize (Zea mays L.) and its responses to abiotic stress. RESULTS: In this study, 85 U-box family proteins were identified in maize and were classified into four subfamilies based on phylogenetic analysis. In addition to the conserved U-box domain, we identified additional functional domains, including Pkinase, ARM, KAP and Tyr domains, by analyzing the conserved motifs and gene structures. Chromosomal localization and collinearity analysis revealed that gene duplications may have contributed to the expansion and evolution of the U-box gene family. GO annotation and KEGG pathway enrichment analysis identified a total of 105 GO terms and 21 KEGG pathways that were notably enriched, including ubiquitin-protein transferase activity, ubiquitin conjugating enzyme activity and ubiquitin-mediated proteolysis pathway. Tissue expression analysis showed that some ZmPUB genes were specifically expressed in certain tissues and that this could be due to their functions. In addition, RNA-seq data for maize seedlings under salt stress revealed 16 stress-inducible plant U-box genes, of which 10 genes were upregulated and 6 genes were downregulated. The qRT-PCR results for genes responding to abiotic stress were consistent with the transcriptome analysis. Among them, ZmPUB13, ZmPUB18, ZmPUB19 and ZmPUB68 were upregulated under all three abiotic stress conditions. Subcellular localization analysis showed that ZmPUB19 and ZmPUB59 were located in the nucleus. CONCLUSIONS: Overall, our study provides a comprehensive analysis of the U-box gene family in maize and its responses to abiotic stress, suggesting that U-box genes play an important role in the stress response and providing insights into the regulatory mechanisms underlying the response to abiotic stress in maize.
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Ubiquitina-Proteína Ligasas , Zea mays , Zea mays/metabolismo , Filogenia , Ubiquitina-Proteína Ligasas/genética , Ubiquitina-Proteína Ligasas/metabolismo , Perfilación de la Expresión Génica , Estrés Fisiológico/genética , Ubiquitinas/genética , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas/metabolismo , Familia de MultigenesRESUMEN
Objectives: To describe the clinical feature of a very recently identified phenotype associated with ATAD3A variation. Methods: A neonate with Harel-Yoon syndrome was identified. We describe the proband's clinical and radiological features. The affected newborn and her parents underwent whole-exome sequencing and PCR-Sanger sequencing. Results: Previously reported clinical manifestations were rare in the neonatal period, including unmanageable seizures necessitating the use of multiple drugs, congenital laryngeal stridor, hypotonia, challenges with feeding, corneal opacity, and subsequent demise due to respiratory failure. Molecular investigations have unveiled the presence of a newly identified heterozygous single-base substitution (c.1517A > C; p.Q506P) within the ATAD3A gene. Discussion: This study unveils a novel single-base substitution, thereby expanding the mutation spectrum associated with ATAD3A. Furthermore, the clinical characteristics exhibited during the neonatal phase are comprehensively described, potentially facilitating improved clinical recognition of ATAD3A-associated HAYOS.
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The effect of soybean oil (SO) on freeze-thaw (F-T)-treated surimi was investigated and its related mechanism was revealed by molecular dynamics (MD) simulations. The results displayed that SO has a disrupting effect on the structure of fresh samples. However, in the F-T-treated samples, surimi gels supplemented with SO had a more uniform microstructure. Simultaneously, when SO was added from 0% to 7% in the F-T-treated samples, the gel strength increased from 46.66 to 51.86 N · mm $$ 46.66\ \mathrm{to}\ 51.86\;\mathrm{N}\cdotp \mathrm{mm} $$ (p < .05), the physically bound water was increased from 92.90% to 94.15% (p < .05), and storage modulus was increased from 5939 to 6523 Pa. Triglycerides of SO generated hydrophobic interactions with myosin mainly in carbon chains. Computational results from MD simulations illustrated that the structure of myosin combined with triglycerides was more stable than that of myosin alone during temperature fluctuations (-20 to 4°C). During ice crystal growth, triglycerides absorbed on the myosin surface inhibited the growth of surrounding ice crystals and mitigated the ice crystal growth rate (from 7.54 to 5.99 cm/s). The addition of SO during the F-T treatments allowed myosin to be less negatively affected by ice crystal formation and temperature fluctuations and ultimately contributed to the formation of a more uniform network gel structure.
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The models of preserved egg yolk (PEY) and salted egg yolk both treated with or without NaCl were performed to explore the effect of NaCl on the characteristic volatile compounds (VOCs) in PEY. 1-hexanol, 2-heptanone, isoamyl acetate, etc., compounds were confirmed as the characteristic VOCs in PEY mainly induced by NaCl and the formation of 1-octanol, 2-pentylfuran, ammonia, etc., characteristic VOCs induced by NaCl may depend on the combined effect of Cu2+ and OH-. Among them, 1-hexanol and 2-heptanone were formed from linoleic acid in PS(18:0_18:2) and oleic acid in PG(22:6_18:1), respectively, through multi-omics and correlation analysis. Meanwhile, 1-octanol may originated from ß-oxidation of oleic acid in PS(18:1); 2-pentylfuran and ammonia maybe derived from the derivative of aspartate and the degradation of l-methionine, respectively. Moreover, this study provides a new insight to parse the influence of NaCl with/without other exogenous factors on the formation of VOCs in food products.
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Amoníaco , Multiómica , 1-Octanol , Yema de Huevo , Ácido Oléico , Cloruro de SodioRESUMEN
In order to have a clearer understanding of the sources of ozone pollution in Yulin City in summer and put forward scientific governance suggestions, the WRF-CMAQ model was used to simulate the O3 concentration in Yulin City and surrounding areas (including Taiyuan City, Xi'an City, Yinchuan City, Hohhot City, and other provincial capital cities) in July 2019. Using the ISAM module, the sources of O3 and its precursors NOx and VOCs in a heavy pollution process in Yulin City were quantified. The results showed that on heavy pollution days, the O3 in Yulin City mainly came from the long-distance transmission outside the simulation area (55.5%), followed by the photochemical reaction of precursors in the simulation area (20.6%, 10.0%, 5.0%, 2.3%, and 2.1%, respectively, in Yulin City, Shanxi Province, Inner Mongolia Autonomous Region, and Shaanxi Province, 1.2% in Gansu Province, Ningxia Hui Autonomous Region, and Henan Province in total), and initial conditions (0.3%); the remaining sources (23.6%) could not be successfully labeled. Yulin City is in the VOCs control area, and its VOCs were composed of paraffin (76.5%), ketones (9.2%), and other types of VOCs (14.3%). The VOCs came from the emission of pollution sources in the simulation area (45.6%, 22.0%, 11.4%, 6.3%, and 5.1%, respectively, in Yulin City, Shanxi Province, Inner Mongolia Autonomous Region, and Shaanxi Province, 0.8% in Gansu Province, Ningxia Hui Autonomous Region, and Henan Province in total) and the long-distance transmission outside the simulation area (27.9%); the remaining 26.5% were not successfully marked. This research showed that to control the O3 pollution in Yulin, not only should the local VOCs emissions be controlled, but the overall planning of VOCs emissions in the peripheral areas should also be done well.
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As a typical coastal city, O3 pollution in Rizhao has become increasingly serious in recent years. In order to explore the causes and sources of O3 pollution, IPR process analysis and ISAM source tracking tools based on the CMAQ model were used, respectively, to quantify the contributions of different physicochemical processes and different source tracking areas to O3 in Rizhao. Additionally, by comparing the differences between O3-exceeding days and non-exceeding days, combined with the HYSPLIT model, the regional transportation path of O3 in Rizhao was explored. The results showed that the concentrations of O3, NOx, and VOCs near the coastal areas of Rizhao and Lianyungang were significantly increased on O3 exceedance days compared with those on non-exceedance days. This was mainly because Rizhao was the convergence zone of western, southwestern, and eastern winds on exceedance days, which facilitated the transport and accumulation of pollutants. Process analysis showed that the transport process (TRAN) contribution to the near-surface O3 near the coastal areas of Rizhao and Lianyungang increased significantly on the exceedance days, whereas the contribution to most areas to the west of Linyi decreased. Photochemical reaction (CHEM) had a positive contribution to the O3 concentration in Rizhao during the daytime at all heights, and TRAN had a positive contribution at 0-60 m above the ground, and mainly had a negative contribution above 60 m. The contributions of CHEM and TRAN at 0-60 m above the ground would increase significantly on exceedance days, which was approximately twice that on the non-exceedance days. Source analysis showed that the local sources in Rizhao were the main contribution sources of NOx and VOCs, with the contribution rates of 47.5% and 58.0%, respectively. O3 mainly came from the contribution outside the simulation area (67.5%). The O3 and precursor contributions of the western cities of Rizhao (Weifang, Linyi, etc.) and the southern cities (Lianyungang, etc.) would increase significantly on the days of exceeding the standard. The transportation path analysis showed that the number of exceedances accounted for the largest proportion (11.8%) in the path from the west of Rizhao, which was the main transportation channel of O3 and precursors in Rizhao. This was verified through process analysis and source tracking results, and such trajectories accounted for 13.0% of the total number of trajectories, and their main routes were in the Shaanxi, Shanxi, Hebei, and Shandong regions.
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Zhayu is a type of traditional fermented fish product in China that is made through the fermentation of salted fish with a mixture of cereals and spices. Inoculation fermentation was performed using Pediococcus pentosaceus P1, Lactiplantibacillus plantarum L1, and a mixture of two strains, which were isolated from cured fish in Hunan Province. Compared with the natural fermentation, inoculation with lactic acid bacteria (LAB) accelerated the degradation of myosin and actin in Zhayu, increased the trichloroacetic acid (TCA)-soluble peptide content by about 1.3-fold, reduced the colony counts of Enterobacteriaceae and Staphylococcus aureus by about 40%, and inhibited their lipid oxidation. In the texture profile analysis performed, higher levels of hardness and chewiness were observed in the inoculation groups. In this study, the bacterial community and volatile flavor compounds were detected through 16S high-throughput sequencing and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). Inoculation with L. plantarum L1 reduced around 75% abundance of Klebsiella compared with the natural fermentation group, which was positively correlated with 2,3-Butanediol, resulting in a less pungent alcohol odor in Zhayu products. The abundances of 2-pentylfuran and 2-butyl-3-methylpyrazine were increased over threefold in the L1 group, which may give Zhayu its unique flavor and aroma.
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This research was aimed to quantify the effects of acetic acid, malic acid, and citric acid (0, 0.5, 1.0, and 2.0 g/100 g H2 O) on the stress-strain responses of fish gelatin (FG) gels (2, 4, and 6.67 g/100 g H2 O) under uniaxial compression up to 68% of deformation. The first-order Ogden model fitted quite well for the compression responses of FG gels (R2 = 0.9909-0.9997). Protons from the acids played a key role on weakening the FG gel structures (gel rigidity, µ, decreased 11%-27%), as the µ values and pH values of FG gels were linearly correlated (R2 = 0.8240-0.9748), regardless of the acid type. The addition of an acid also resulted in a significant increase (p < .002) in the strain hardening capacity (α) of gels with 2 g FG/100 g H2 O. Both µ and α values of FG gels with higher gelatin concentrations were less affected by an acid partly due to their stronger buffering effects. The µ and α values of FG gels as affected by acids could not be fully explained based upon the pH changes, implying that the effects of acetate, malate, and citrate ions on the gel structure could not be ignored.
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OBJECTIVE: To investigate risk factors for metabolic bone disease (MBD) in preterm infants and establish a nomogram model for predicting MBD risk. METHODS: A total of 1104 preterm infants were enrolled, among whom 809 were included in the modelling set and 295 were included in the validation set. The modelling set was divided into MBD (n = 185) and non-MBD (n = 624) groups. A multivariate logistic regression analysis was used to investigate the independent risk factors for MBD. R software was used to plot the nomogram model, which was then validated by the data of the validation set. Receiver operating characteristic (ROC) and calibration curves were used to evaluate the nomogram model's performance, and the clinical decision curve was used to assess the clinical practicability of the model. RESULTS: Gestational age, time of trophic feeding initiation, parenteral nutrition duration, necrotizing enterocolitis, bronchopulmonary dysplasia, cholestasis and sepsis were independent risk factors for MBD in preterm infants (P < 0.05). The ROC curve of the modelling set had an area under the curve (AUC) of 0.801; the risk prediction value of 0.196 corresponding to the maximum Youden index was the best value, and the prediction critical value was 125 points. The ROC curve of the validation set had an AUC of 0.854. The calibration curve analysis showed good accuracy and consistency between the model's predicted and actual values. CONCLUSIONS: The nomogram model provides an efficient tool for the early assessment of MBD risk. Preterm infants with scores ≥ 125 should receive close attention and interventions in the early stage. WHAT IS KNOWN: ⢠The incidence and severity of MBD are inversely proportional to gestational age and birth weight. Bone loss can lead to prolonged hospital stay, ventilator dependence, pathological fractures and short stature. WHAT IS NEW: ⢠Gestational age, time of trophic feeding initiation, parenteral nutrition duration, necrotizing enterocolitis, bronchopulmonary dysplasia, cholestasis and sepsis were independent risk factors for MBD in preterm infants. The nomogram model provides an efficient tool for the early assessment of MBD risk.
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Enfermedades Óseas Metabólicas , Displasia Broncopulmonar , Colestasis , Enterocolitis Necrotizante , Enfermedades del Recién Nacido , Lactante , Recién Nacido , Humanos , Recien Nacido Prematuro , Estudios de Casos y Controles , Nomogramas , Estudios Retrospectivos , Enterocolitis Necrotizante/diagnóstico , Enterocolitis Necrotizante/epidemiología , Enterocolitis Necrotizante/etiología , Enfermedades Óseas Metabólicas/diagnóstico , Enfermedades Óseas Metabólicas/epidemiología , Enfermedades Óseas Metabólicas/etiologíaRESUMEN
The membrane-separated silver carp hydrolysates (>10 kD, 3-10 kD and < 3 kD) displayed abilities to mitigate oxidation and denaturation of myofibrillar protein and cryoprotective activities for frozen surimi. However, the mechanism of the membrane-separated fractions on ice crystal growth in the system is still unknown. Therefore, the cryoprotective activities (recrystallization inhibition, RI and thermal hysteresis activity, THA) of the fractions were investigated and the mechanism was explored by molecular dynamics (MD) simulation to predict the probable binding sites and model the possible interactions between the peptides and water/ice. The fractions < 3 kD displayed remarkable RI activity, with significantly higher THA (0.60 ± 0.13 °C) and lower amount of ice nuclei (4.74 ± 0.53%) than that of fractions > 10 kD and 3-10 kD. The results of MD simulation certified that the main peptides in the fractions < 3 kD interacted firmly with water molecules and inhibited growth of ice crystals with mechanism compatible with Kelvin effect. Hydrophilic and hydrophobic amino acid residues in the membrane-separated fractions offered synergistic effects on the inhibition of ice crystals.
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Carpas , Hielo , Animales , Simulación de Dinámica Molecular , Cristalización , Congelación , Agua/química , Péptidos/química , Proteínas Anticongelantes , Crioprotectores/farmacologíaRESUMEN
This study aimed to screen and characterize antifreeze peptides from silver carp muscle hydrolysate (SCMH). The SCMH was initially fractionated by ultrafiltration, and the resultant SCMH-IV (<10 kDa) showing 90 % of yeast survival rate was subsequently separated into four fractions using ion-exchange chromatography. The fraction with the best antifreeze activity was further analyzed by liquid chromatography-tandem mass spectrometry. A total of 514 peptides were identified, of which a novel antifreeze peptide (Sc-AFP, KAADSFNHKAFFAKVG) with a thermal hysteresis activity of 0.87 â was selected. The parvalbumin-derived Sc-AFP showed an alanine-rich, α-helical and amphipathic character. Based on molecular dynamics simulations, the Sc-AFP could interact with 48 water molecules via hydrogen bonds, and could be adsorbed onto the ice surface through a total of 21 hydrogen bonds mainly linked to the lysine residues. This could account for its antifreeze properties via preventing the formation and growth of ice crystals.
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Carpas , Hielo , Animales , alfa-Fetoproteínas , Proteínas Anticongelantes/química , MúsculosRESUMEN
The heat-induced (121 °C, 10 or 30 min) formation of two potentially hazardous advanced glycation end-products (AGEs), protein-bound NÉ -carboxymethyllysine (CML) and NÉ -carboxyethyllysine (CEL), in pork as affected by citric or acetic acid (0.5, 1 g/100 pork) and the storage duration (0 °C, 0 - 8 d) prior to the heating was investigated. A longer storage time of raw pork resulted in higher levels of AGEs produced during the later heating, likely due to the accumulation of some AGE precursors during the storage. Depending on the acid level and heating time, adding acid in pork led to 30 - 54% (citric acid) or 14 - 48% (acetic acid) average reduction of heat-induced production of CML/CEL, which corresponded to the reduction of thiobarbituric acid reactive substances and Schiff bases. The marinating time of raw pork with an acid did not significantly affect (P = 0.959 - 0.998) the acid's inhibition effect on heat-induced formation of CML/CEL.
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Lipid oxidation and protein degradation, along with the formation of advanced glycation end-products (AGEs), including Nε-carboxymethyl-lysine (CML), Nε-carboxyethyl-lysine (CEL) and fluorescent AGEs, in raw and subsequently heated surimi products were investigated during freezing-thawing cycles. Lipid oxidation, protein degradation, Schiff base, and AGEs formation constantly increased during freezing-thawing cycles and heat treatment (P < 0.05). Heat-induced increase of AGEs in surimi products was accelerated by freezing-thawing treatment. Formation of CML in one-stage heated (45 °C, 30 min) samples increased from 0.10 ± 0.04 to 0.53 ± 0.11 mg/kg and CEL increased from 0.03 ± 0.32 to 0.92 ± 0.74 mg/kg. In two-stage heated samples (45 °C, 30 min and 90 °C, 20 min), CML increased from 0.86 ± 0.06 to 1.12 ± 0.11 mg/kg and CEL from 1.00 ± 0.34 to 2.11 ± 1.86 mg/kg, during up to 6 freezing-thawing cycles. Formation of fluorescent AGEs derived from heating was also significantly accelerated by freezing-thawing treatment (P < 0.01). Correlation analysis suggested that the chemical synthesis of AGEs in surimi products was promoted by lipid oxidation and protein degradation during freezing-thawing cycles. AGEs formation through Schiff base oxidation likely occurred only under thermal treatment since no relationship was found between Schiff base and AGEs levels in raw surimi products.
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Carpas , Productos Finales de Glicación Avanzada , Animales , Carpas/metabolismo , Congelación , Productos Finales de Glicación Avanzada/análisis , Calor , Lípidos , Bases de SchiffRESUMEN
There is a huge demand to identify the sex of unhatched fertilized eggs for laying industry and to understand the differences between male and female eggs as early as possible. Then the molecular mechanisms of sex determination and sex allocation in chicken were revealed. Therefore, TMT proteomic was applied to characterize the variation of molecular matrix between unhatched male and female egg yolks. A total of 411 proteins were identified and 35 differentially expressed proteins (DEPs), including 375332005, 015809562, 763550308 (upregulated, UPs) and 1337178851, 89000557, 89000581 (downregulated, DPs), etc. were confirmed between them. Gene ontology analyses showed that DEPs were mainly involved in response to stimulus, distributed in the extracellular region and participated in binding; KEGG analyses showed that few DPs were participated in cell growth and death, transport and catabolism, signaling molecules, interaction and were enriched in ubiquitin mediated proteolysis, endocytosis, ferroptosis, etc. metabolic pathways. Moreover, most of the DEPs and related metabolic pathways were associated with sex hormones. More importantly, this study supports maternal sex-allocation theory and extends our understanding of the molecular mechanism of sex determination and differentiation in avian. Which also provides a powerful evidence for ovo sexing of unhatched fertilized domestic chicken eggs by nondestructive approach and will be of great significance to eggs processing and production.
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Pollos , Yema de Huevo , Animales , Pollos/genética , Yema de Huevo/química , Femenino , Masculino , Óvulo , Proteómica , Análisis para Determinación del Sexo/veterinariaRESUMEN
Structural evolution, digestibility and inhibition on starch digestion of rice glutelin fibril aggregates (RGFAs) as affected by incubation were investigated. Thioflavin T fluorescence intensity of the RGFAs, incubated for 4-day, reached the maximum values, which ranged from 845.00 ± 23.52 to 873.67 ± 50.30. Transmission electron microscopy (TEM) observed that the samples heated for 2 h (2 h fibril) were self-assembled from small glutelin aggregates and a few protofibrils into mature fibrils, the samples heated for 4-10 h (4-10 h fibril) were elongated into long, branched fibrils, and the longer fibrils of 15 h fibril sample dissociated into short fibrils after 4-day of incubation. Compared to rice glutelin, the RGFAs showed thermal stability and resistance to proteolysis. The fluorescence retention rate of 6 h fibril, after incubation for 4-day, was 8.62 ± 0.61 % after in vitro stomach and pancreas digestion, which was the highest among all of the samples. The RGFAs incubated for 1-day displayed much better inhibition effects on starch digestion. This was the first study to clarify the relationship between incubation and physicochemical/functional properties of protein fibrils, which could help understand the preservation of food protein fibrils and their application.
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Oryza , Almidón , Digestión , Glútenes , Microscopía Electrónica de Transmisión , Oryza/química , Almidón/químicaRESUMEN
Measurements of ozone (O3) and its precursors were performed in the summer of 2019 in Lanzhou, a petrochemical industrial city, to better understand the reactivity of volatile organic compounds (VOCs) and their effects on O3 production. During the campaign, the daily maximum 8-hour average (MDA8) O3, NO2, and total VOC (TVOC) concentrations reached 72.2 ± 19.9 ppb, 24.9 ± 10.8 ppb, and 50.8 ± 46.1 ppb, respectively. Alkanes, alkenes, halocarbons, aromatics, and alkynes contributed 45.3%, 24.0%, 16.5%, 10.0%, and 4.2% to TVOCs, respectively. The OH reactivity and relative incremental reactivity (RIR) of VOCs at different times were calculated. The results indicated that alkenes played a predominant role, accounting for an average of 68.5% of the initial VOC reactivity. Compared to other regions, alkenes are relatively more important for O3 formation in the petrochemical industry area of Lanzhou, while aromatics are relatively less important. Generally, O3 formation occurred in a VOC-limited regime in the morning and in a transitional regime in the afternoon. The response surface methodology (RSM) combined with a chemical box model was applied to obtain relationships between O3 and its precursors and determine the most effective way to reduce the O3 concentration. Reduction in the non-alkene concentration slightly affected the O3 concentration. In contrast, the effect of nitrogen oxides (NOx) was closely related to the alkene concentration, and NOx concentration reduction could lead to an increase in the O3 concentration when alkenes were abated to less than 80% of the present concentration. To mitigate O3 pollution near the petrochemical industrial area of Lanzhou, reducing the alkene concentration, especially the C4 alkene concentration (1,3-butadiene, cis-2-butene, and trans-2-butene), was the fastest and most effective control strategy. The results of this study serve as a reference for O3 pollution control in petrochemical industrial areas.
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Contaminantes Atmosféricos , Ozono , Compuestos Orgánicos Volátiles , Contaminantes Atmosféricos/análisis , Alquenos , China , Monitoreo del Ambiente/métodos , Ozono/análisis , Compuestos Orgánicos Volátiles/análisisRESUMEN
A new electrochemical sensor has been constructed for ultra-sensitive detection of lead ions (Pb2+) by square wave anodic stripping voltammetry (SWASV), based on the copper sulfide/graphitic carbon nitride nanocomposite modified glassy carbon electrode (CuS/g-C3N4/GCE). First, spherical CuS nanoparticles with good electrical conductivity were anchored on layered g-C3N4 with high coordination activity, affording an excellent electrode modifier CuS/g-C3N4 nanocomposite. Then, the performance of the CuS/g-C3N4/GCE and its electrochemical response to Pb2+ were thoroughly studied, and the sensing mechanism was investigated. On the one hand, the CuS/g-C3N4 nanocomposite has greatly improved the electron transportation and electrode performance through functional complementarity - CuS endows g-C3N4 with a good electrical conductivity and a large active specific surface area, while g-C3N4 endows CuS with high dispersibility and strong adsorption. On the other hand, the CuS/g-C3N4 modifier has effectively promoted the deposition of trace Pb2+ from the solution onto the electrode surface by means of synergistic enrichment (crucial for amplification of detection signals) - g-C3N4 can coordinate with Pb2+ by its large number of conjugated triazine heterocyclic rings in its molecular framework, while CuS can adsorb Pb2+ due to its inherent size effect of nanomaterials. The proposed sensor can efficiently detect Pb2+ in the concentration range of 0.050-5.000 µM with a limit of detection (LOD) as low as 4.00 nM, and can be well applied for the detection of trace Pb2+ in actual tea samples.