Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Más filtros











Base de datos
Intervalo de año de publicación
1.
J Clin Microbiol ; 42(5): 2227-30, 2004 May.
Artículo en Inglés | MEDLINE | ID: mdl-15131197

RESUMEN

We describe a 7-month outbreak of nosocomial Burkholderia cepacia bacteremia involving eight children in a pediatric hospital and the results of epidemiological investigations. A B. cepacia strain genotypically identical to the blood isolates was recovered from the upper surface of capped rubber stoppers of bottles of a commercial lipid emulsion used for parenteral nutrition.


Asunto(s)
Bacteriemia/epidemiología , Infecciones por Burkholderia/epidemiología , Burkholderia cepacia , Infección Hospitalaria/epidemiología , Brotes de Enfermedades , Contaminación de Equipos , Bacteriemia/microbiología , Infecciones por Burkholderia/microbiología , Burkholderia cepacia/clasificación , Burkholderia cepacia/genética , Burkholderia cepacia/aislamiento & purificación , Infección Hospitalaria/microbiología , Emulsiones , Humanos , Lactante , Recién Nacido , Lípidos , Nutrición Parenteral/efectos adversos , Nutrición Parenteral/instrumentación , Paris/epidemiología , Ribotipificación
2.
Antimicrob Agents Chemother ; 45(6): 1889-91, 2001 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-11353645

RESUMEN

Macrolide susceptibility was investigated in clinical group B streptococci obtained from neonates or pregnant women in 2000 in France. Of 490 consecutive isolates, 18% were resistant to erythromycin. The erm(B), erm(A) subclass erm(TR), and mef(A) genes were harbored by 47, 45, and 6% of these strains, respectively. Two isolates did not harbor erm or mef genes.


Asunto(s)
Antibacterianos/farmacología , Metiltransferasas/genética , Streptococcus agalactiae/efectos de los fármacos , Farmacorresistencia Microbiana/genética , Femenino , Humanos , Recién Nacido , Macrólidos , Pruebas de Sensibilidad Microbiana , Embarazo , Streptococcus agalactiae/genética , Streptococcus agalactiae/aislamiento & purificación
3.
J Infect Dis ; 181(6): 1971-8, 2000 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-10837177

RESUMEN

The genetic diversity of Streptococcus pneumoniae isolates (n=291) recovered from cerebrospinal fluid of patients with meningitis in France was investigated by restriction fragment length polymorphism analysis of the ribosomal RNA gene regions and of the pbp2b and 2x genes. Statistical analysis of the data by factorial analysis of correspondence established the following: penicillin-susceptible isolates had a high level of genetic diversity, especially those belonging to serogroups frequently associated with carriage; capsular serotype switches could occur among penicillin-susceptible and -resistant isolates; and the mechanisms of acquired penicillin resistance were clearly distinct in isolates with penicillin minimum inhibitory concentration (MIC) values <1 mg/L and isolates with penicillin MIC values >/=1 mg/L. Thus, an increase in the penicillin MIC for a given strain, from intermediate to high-level resistance would be a rare event.


Asunto(s)
Aminoaciltransferasas , Proteínas Bacterianas , Hexosiltransferasas , Meningitis Neumocócica/microbiología , Resistencia a las Penicilinas , Peptidil Transferasas , Streptococcus pneumoniae/efectos de los fármacos , Streptococcus pneumoniae/genética , Adulto , Proteínas Portadoras/genética , Niño , Variación Genética , Humanos , Meningitis Neumocócica/tratamiento farmacológico , Muramoilpentapéptido Carboxipeptidasa/genética , Proteínas de Unión a las Penicilinas , Polimorfismo de Longitud del Fragmento de Restricción , Streptococcus pneumoniae/clasificación
4.
Antimicrob Agents Chemother ; 44(6): 1453-7, 2000 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-10817692

RESUMEN

A total of 1,500 recent throat isolates of Streptococcus pyogenes collected between 1996 and 1999 from children throughout France were tested for their susceptibility to erythromycin, azithromycin, josamycin, clindamycin, and streptogramin B. The erythromycin-resistant isolates were further studied for their genetic mechanism of resistance, by means of PCR. The clonality of these strains was also investigated by means of serotyping and ribotyping. In all, 6.2% of the strains were erythromycin resistant, and 3.4 and 2.8% expressed the constitutive MLS(B) and M resistance phenotypes and harbored the ermB and mefA genes, respectively; ermTR was recovered from one isolate which also harbored the ermB gene. Ten serotypes and 8 ribotypes were identified, but we identified 17 strains by combining serotyping with ribotyping. Among the eight ribotypes, the mefA gene was recovered from six clusters, one being predominant, while the ermB gene was recovered from four clusters, of which two were predominant.


Asunto(s)
Antibacterianos/farmacología , Farmacorresistencia Microbiana , Eritromicina/farmacología , Infecciones Estreptocócicas/tratamiento farmacológico , Streptococcus pyogenes/efectos de los fármacos , Antibacterianos/uso terapéutico , Niño , Preescolar , Eritromicina/uso terapéutico , Francia/epidemiología , Humanos , Lactante , Infecciones Estreptocócicas/epidemiología , Infecciones Estreptocócicas/microbiología
5.
Scand J Gastroenterol ; 35(2): 160-5, 2000 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-10720113

RESUMEN

BACKGROUND: Small-bowel resection in animals results in alterations of the morphology and functional adaptation in the remaining intestine. The aim of our study was to study the effect of Saccharomyces boulardii versus placebo in rats after 50% small-bowel resection. METHODS: Sixty-three rats were assigned to one of three groups: small-bowel resection (n = 31), transected surgery controls (n = 16), or non-surgical controls (n = 16). Of the 31 rats with small-bowel resection, 15 were given S. boulardii (140 mg/dl), and 16 were given placebo. Intestinal markers measured included bacterial overgrowth (BO) on days 4 and 8 and translocation into mesenteric lymph nodes, liver, and spleen. Markers of small-bowel adaptation included histomorphology of the mucosa, protein content, and various brush-border enzymes (sucrase, glucoamylase, n-aminopeptidase). RESULTS: In the jejunal mucosal samples on day 8, S. boulardii-treated rats showed a significant increase in protein content (58.3 +/- 12 mg/10 cm) compared with placebo-treated rats (29.2 +/- 1.8) or non-surgery controls (18.3 +/- 1.2; P < 0.001). S. boulardii-treated rats also had significantly higher levels of all three brush-border enzymes. A significant increase of enzyme-specific activities was observed in the ileum of S. boulardii resected rats compared with the placebo resected group on day 4, and no significant differences were seen in the remnant ileum except an increase in protein content in S. boulardii-treated rats on day 8. Histomorphometric studies showed no differences in ileal villus height or translocation frequencies by day 8 in S. boulardii or placebo resected rats. CONCLUSIONS: These data indicate that, after resection, S. boulardii does not modify bacterial overgrowth or translocation frequency but does significantly enhance the functional adaptation of the remaining intestinal segments.


Asunto(s)
Traslocación Bacteriana , Intestino Delgado/microbiología , Intestino Delgado/cirugía , Saccharomyces , Adaptación Fisiológica , Animales , Fenómenos Fisiológicos Bacterianos , Enzimas/metabolismo , Intestino Delgado/fisiología , Masculino , Ratas , Ratas Sprague-Dawley
6.
Antimicrob Agents Chemother ; 43(6): 1480-3, 1999 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-10348775

RESUMEN

The genetic relatedness of French isolates of Streptococcus pneumoniae highly resistant to amoxicillin (MIC, >/=4 microg/ml, equal to or exceeding those of penicillin) was investigated by molecular fingerprinting. The results suggest that high-level resistance to amoxicillin has emerged within preexisting penicillin-resistant clones.


Asunto(s)
Amoxicilina/farmacología , Resistencia a las Penicilinas , Penicilinas/farmacología , Streptococcus pneumoniae/efectos de los fármacos , Dermatoglifia del ADN , Humanos , Pruebas de Sensibilidad Microbiana , Polimorfismo de Longitud del Fragmento de Restricción , Serotipificación , Streptococcus pneumoniae/clasificación , Streptococcus pneumoniae/genética
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA