Facilitating Growth of Maize (Zea mays L.) by Biostimulants: A Perspective from the Interaction between Root Transcriptome and Rhizosphere Microbiome.

The plant growth-promoting effects of biostimulants have been widely documented, while little is known about the intrinsic mechanism. In our study, a pot experiment was conducted to investigate the effects of biostimulants on maize, and the maize root transcriptome and rhizosphere microbiome were assessed. The physicochemical properties of the soil were significantly altered with various trends, and the growth and yield of maize were promoted by biostimulants. Sampling time and maize strain were the strongest factors that altered the rhizosphere microorganisms. Rhizosphere microbiota with biostimulant application exhibited high community robustness. Root transcriptome analysis suggested an altered expression profile induced by biostimulants and maize strains. An integrated correlation analysis demonstrated that phosphate and nitrate metabolism genes are tightly associated with some rhizosphere microbiota. These results implied the plant growth-promoting effects of biostimulants might act in a rhizosphere microorganism-dependent manner and help to expand the use of biostimulants in sustainable agriculture.

Estimation of Botanical Composition in Mixed Clover-Grass Fields Using Machine Learning-Based Image Analysis.

This study aims to provide an effective image analysis method for clover detection and botanical composition (BC) estimation in clover-grass mixture fields. Three transfer learning methods, namely, fine-tuned DeepLab V3+, SegNet, and fully convolutional network-8s (FCN-8s), were utilized to detect clover fractions (on an area basis). The detected clover fraction (CF detected ), together with auxiliary variables, viz., measured clover height (H clover ) and grass height (H grass ), were used to build multiple linear regression (MLR) and back propagation neural network (BPNN) models for BC estimation. A total of 347 clover-grass images were used to build the estimation model on clover fraction and BC. Of the 347 samples, 226 images were augmented to 904 images for training, 25 were selected for validation, and the remaining 96 samples were used as an independent dataset for testing. Testing results showed that the intersection-over-union (IoU) values based on the DeepLab V3+, SegNet, and FCN-8s were 0.73, 0.57, and 0.60, respectively. The root mean square error (RMSE) values for the three transfer learning methods were 8.5, 10.6, and 10.0%. Subsequently, models based on BPNN and MLR were built to estimate BC, by using either CF detected only or CF detected , grass height, and clover height all together. Results showed that BPNN was generally superior to MLR in terms of estimating BC. The BPNN model only using CF detected had a RMSE of 8.7%. In contrast, the BPNN model using all three variables (CF detected , H clover , and H grass ) as inputs had an RMSE of 6.6%, implying that DeepLab V3+ together with BPNN can provide good estimation of BC and can offer a promising method for improving forage management.