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OBJECTIVE: This study aims to develop and validate a risk nomogram for urinary tract infections (UTIs) in geriatric patients with hip fractures. PATIENTS AND METHODS: A total of 900 geriatric patients who underwent hip fracture surgery at Dandong Central Hospital between June 2017 and June 2023 were systematically collected. The cohort was randomly divided into a training set (70%, n=632) and a validation set (30%, n=268) for model development and validation, respectively. Univariate and multivariate logistic regression analyses were conducted to identify the independent risk factors associated with UTIs. Based on the results of the multivariate analysis, a UTI nomogram prediction model was developed and evaluated in the training and validation sets using the C-index, ROC curve, calibration curve, and decision curve analysis to assess discrimination, calibration, and clinical utility, respectively. RESULTS: Out of the 900 participants, 24.6% were diagnosed with UTIs. The nomogram was developed based on 9 predictors that were found to be independently associated with UTI. The area under the curve (AUC) for predicting UTI in geriatric patients with hip fractures was 0.829 in the training set and 0.803 in the validation set. Following internal verification, the modified C-index remained at 0.829. Furthermore, the nomogram's calibration plot and decision curve analysis demonstrated good performance in both the training and validation sets. CONCLUSIONS: The established and validated nomogram provides a reliable and convenient tool for predicting UTI risk in geriatric patients with hip fractures. This model facilitates the early identification of high-risk patients and offers guidance for implementing targeted preventive interventions.
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Fracturas de Cadera , Infecciones Urinarias , Humanos , Anciano , Estudios Retrospectivos , Nomogramas , Fracturas de Cadera/cirugía , Área Bajo la Curva , Infecciones Urinarias/diagnóstico , Infecciones Urinarias/epidemiologíaRESUMEN
OBJECTIVE: 30-day readmission after hip fracture surgery in the elderly is common and costly. A predictive tool to identify high-risk patients could significantly improve outcomes. This study aims to develop and validate a risk nomogram for 30-day readmission after hip fracture surgery in geriatric patients. PATIENTS AND METHODS: We retrospectively analyzed 1,249 geriatric hip fracture patients (≥60 years) undergoing surgery at Dandong Central Hospital from October 2011 to October 2023. Using a 7:3 ratio, patients were randomly divided into training (n=877) and validation (n=372) sets. Independent risk factors for 30-day readmission were identified using LASSO regression and logistic regression in the training set. A nomogram was constructed using the identified predictors. Finally, the C-index, ROC curve, calibration curve, and decision curve analysis were used to validate the model in the training and validation sets respectively. RESULTS: The nomogram was developed based on the 8 predictors of age, prior stroke, chronic liver disease, treatment, uric acid (UA), total protein (TP), albumin (ALB), and pneumonia that were found to be independently associated with 30-day readmission. The nomogram showed good discrimination with a C-index of 0.88 in the training set and 0.84 in the validation set. Calibration curves exhibited good agreement between predicted and observed outcomes. Decision curve analysis demonstrated clinical utility. CONCLUSIONS: We developed and validated a nomogram incorporating eight clinical variables to accurately predict the individualized risk of 30-day readmission after hip fracture surgery in elderly patients. The model demonstrated favorable discrimination, calibration, and clinical utility. It can help to identify high-risk patients needing additional interventions to prevent avoidable hospital readmissions.
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Fracturas de Cadera , Readmisión del Paciente , Anciano , Humanos , Albúminas , Fracturas de Cadera/cirugía , Nomogramas , Estudios Retrospectivos , Distribución AleatoriaRESUMEN
OBJECTIVE: This study aimed to develop and validate a risk nomogram for preoperative proximal and distal deep vein thrombosis (DVT) in geriatric patients with hip fractures. PATIENTS AND METHODS: The 970 collected geriatric hip fracture patients were randomly divided into a training set (70%, n=682) and a validation set (30%, n=288). Multivariate logistic regression analyses were used to optimize the predictive risk variables for proximal and distal preoperative lower extremity DVT in the training set, respectively, and the selected variables were finally incorporated to establish preoperative DVT nomogram prediction models. Receiver operating characteristic curves (ROC), calibration plots, and decision curve analysis (DCA) were performed to validate the nomograms in the training and validation sets, respectively. RESULTS: Among the 970 patients, 125 (12.88%) were diagnosed with preoperative DVT. The area under the curve (AUC) for predicting preoperative proximal DVT was 0.888 in the training and 0.792 in the validation sets. The AUC for predicting preoperative distal DVT was 0.907 in the training and 0.790 in the validation sets. The calibration plots and decision curve analysis for preoperative proximal DVT performed well in the training set and slightly worse in the validation set. The calibration plots and decision curve analysis for preoperative distal DVT performed well in both the training and validation sets. CONCLUSIONS: To construct nomograms for predicting the risk of proximal and distal preoperative lower extremity DVT in geriatric hip fracture patients. For patients at high risk, as assessed by this model, clinicians should intervene and treat them promptly before surgery.
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Fracturas de Cadera , Nomogramas , Humanos , Anciano , Fracturas de Cadera/cirugía , Área Bajo la Curva , Calibración , Extremidad Inferior , Estudios RetrospectivosRESUMEN
Several recent attoclock experiments have investigated the fundamental question of a quantum mechanically induced time delay in tunneling ionization via extremely precise photoelectron momentum spectroscopy. The interpretations of those attoclock experimental results were controversially discussed, because the entanglement of the laser and Coulomb field did not allow for theoretical treatments without undisputed approximations. The method of semiclassical propagation matched with the tunneled wave function, the quasistatic Wigner theory, the analytical R-matrix theory, the backpropagation method, and the under-the-barrier recollision theory are the leading conceptual approaches put forward to treat this problem, however, with seemingly conflicting conclusions on the existence of a tunneling time delay. To resolve the contradicting conclusions of the different approaches, we consider a very simple tunneling scenario which is not plagued with complications stemming from the Coulomb potential of the atomic core, avoids consequent controversial approximations and, therefore, allows us to unequivocally identify the origin of the tunneling time delay.
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A setup of a unique x-ray source is put forward employing a relativistic electron beam interacting with two counterpropagating laser pulses in the nonlinear few-photon regime. In contrast to Compton scattering sources, the envisaged x-ray source exhibits an extremely narrow relative bandwidth of the order of 10^{-4}, comparable with an x-ray free-electron laser. The brilliance of the x rays can be an order of magnitude higher than that of a state-of-the-art Compton source. By tuning the laser intensities and the electron energy, one can realize either a single peak or a comblike x-ray source of around keV energy. The laser intensity and the electron energy in the suggested setup are rather moderate, rendering this scheme compact and tabletop size, as opposed to x-ray free-electron laser and synchrotron infrastructures.
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Background The study examines the function of hypoxia-mediated down-regulation of microRNAs (miRNAs) (mir-30c, mir-135a, and mir-27a) in the process of bladder cancer immune escape. Methods Quantitative Real-time PCR (qRT-PCR) was carried out to determine gene expression levels of Drosha and Dicer under hypoxia treatment, while western blotting and flow cytometry were used to determine protein expression. Seven reported miRNAs were identified via qRT-PCR assay. Flow cytometry detection of CD3/CD4/CD8-positive expression and statistics. Enzyme-linked immunosorbent assay (ELISA) detected cellular immune factors content. Cell apoptosis was checked via flow cytometry assay. Luciferase report assay and western blot assays were both used to verify the relationship between miRNAs and Casitas B-lineage lymphoma proto-oncogene b (Cbl-b). The animal model was established and Hematoxylineosin (HE) staining, TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, and immunohistochemistry (IHC) assays were separately used to verify the conclusions. Results The CD3 + /CD4 + expression was increased in the hypoxia group, while CD3 + /CD8 + expression, the cellular immune factors content Interleukin-2 (IL-2) and Tumor Necrosis Factor-α (TNFα) along with the cell apoptosis were suppressed. The protein expression of Cbl-b was found to be up-regulated in the hypoxia group. After constructing the overexpression/ knockdown of Cbl-b in peripheral blood mononuclear cell (PBMC), Cbl-b has been found to promote tumor immune escape in bladder cancer. Furthermore, Cbl-b had been identified as the co-targets of mir-30c, mir-135a, and mir-27a and down-regulation of miRNA biogenesis promotes Cbl-b expression and deactivating T cells in vitro/in vivo. Conclusion Hypoxia-mediated down-regulation of miRNAs biogenesis promotes tumor immune escape in bladder cancer, which could bring much more advance to the medical research on tumors (AU)
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Humanos , Regulación hacia Abajo/inmunología , MicroARNs/metabolismo , Escape del Tumor/inmunología , Hipoxia Tumoral/inmunología , Neoplasias de la Vejiga Urinaria/inmunología , Apoptosis , Modelos Animales de Enfermedad , Regulación Neoplásica de la Expresión Génica , Interleucina-2/metabolismo , Ratones Endogámicos C57BL , Estudios Prospectivos , Proto-Oncogenes/genética , Distribución Aleatoria , Factor de Necrosis Tumoral alfa/metabolismo , Neoplasias de la Vejiga Urinaria/metabolismoRESUMEN
INTRODUCTION: The relation between type of ventilation used in the operating theatre and surgical site infection has drawn considerable attention. It has been reported that there is a possible relationship between the type of ventilation used in the operation theatre and surgical site infection. This meta-analysis was performed to evaluate this relationship. METHODS: Through a systematic literature search up to May 2020, 14 studies describing 590,121 operations, 328,183 were performed under laminar airflow ventilation and 2,611,938 were performed under conventional ventilation. Studies were identified that reported relationships between type of ventilation with its different categories and surgical site infection (10 studies were related to surgical site infection in total hip replacement, 7 in total knee arthroplasties and 3 in different abdominal and open vascular surgery). Odds ratios with 95% confidence intervals were calculated comparing surgical site infection prevalence and type of theatre ventilation using the dichotomous method with a random or fixed-effect model. FINDINGS: No significant difference was found between surgery performed under laminar airflow ventilation and conventional ventilation in total hip replacement (OR 1.23; 95% CI 0.97-1.56, p = 0.09), total knee arthroplasties (OR 1.14; 95% CI 0.62-2.09, p = 0.67) or different abdominal and open vascular surgery (OR 0.75; 95% CI 0.43-1.33, p = 0.33). The impact of the type of theatre ventilation may have no influence on surgical site infection as a tool for decreasing its occurrence. CONCLUSIONS: Based on this meta-analysis, operating under laminar airflow or conventional ventilation may have no independent relationship with the risk of surgical site infection. This relationship forces us not to recommend the use of laminar airflow ventilation since it has a much higher cost compared with conventional ventilation.
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Quirófanos , Procedimientos Quirúrgicos Operativos , Infección de la Herida Quirúrgica/epidemiología , Ventilación/estadística & datos numéricos , Artroplastia de Reemplazo de Cadera , Artroplastia de Reemplazo de Rodilla , Ambiente Controlado , Humanos , Procedimientos Quirúrgicos Vasculares , Ventilación/métodosRESUMEN
Inner Mongolia Cashmere goat is a well-known local cashmere goat breed in China. It is famous for excellent fleece quality and a significant advantage in cashmere yield compared to other cashmere goat breeds. In this study, a genome-wide association study was used to investigate fiber length, fiber diameter, and cashmere yield of 192 Inner Mongolia Cashmere goats using the Illumina GoatSNP52K Beadchip panel. We discovered that four single nucleotide polymorphisms (SNPs) reached genome-wide significance levels. These SNPs were located in some genes, e.g. FGF12, SEMA3D, EVPL, and SOX5, possibly related to fleece traits in Inner Mongolia Cashmere goat. Gene ontology enrichment analysis revealed that these genes were enriched in several biological pathways that were involved in hair follicle development in cashmere goats. In summary, the identified significant SNPs and genes provide useful information to explore genetic mechanisms underlying the variation in fleece traits and genomic selection of Chinese cashmere goat.
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Estudio de Asociación del Genoma Completo/veterinaria , Cabras/genética , Cabello , Animales , China , Ontología de Genes , Polimorfismo de Nucleótido SimpleRESUMEN
BACKGROUND: The study examines the function of hypoxia-mediated down-regulation of microRNAs (miRNAs) (mir-30c, mir-135a, and mir-27a) in the process of bladder cancer immune escape. METHODS: Quantitative Real-time PCR (qRT-PCR) was carried out to determine gene expression levels of Drosha and Dicer under hypoxia treatment, while western blotting and flow cytometry were used to determine protein expression. Seven reported miRNAs were identified via qRT-PCR assay. Flow cytometry detection of CD3/CD4/CD8-positive expression and statistics. Enzyme-linked immunosorbent assay (ELISA) detected cellular immune factors content. Cell apoptosis was checked via flow cytometry assay. Luciferase report assay and western blot assays were both used to verify the relationship between miRNAs and Casitas B-lineage lymphoma proto-oncogene b (Cbl-b). The animal model was established and Hematoxylin-eosin (HE) staining, TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, and immunohistochemistry (IHC) assays were separately used to verify the conclusions. RESULTS: The CD3 + /CD4 + expression was increased in the hypoxia group, while CD3 + /CD8 + expression, the cellular immune factors content Interleukin-2 (IL-2) and Tumor Necrosis Factor-α (TNFα) along with the cell apoptosis were suppressed. The protein expression of Cbl-b was found to be up-regulated in the hypoxia group. After constructing the overexpression/ knockdown of Cbl-b in peripheral blood mononuclear cell (PBMC), Cbl-b has been found to promote tumor immune escape in bladder cancer. Furthermore, Cbl-b had been identified as the co-targets of mir-30c, mir-135a, and mir-27a and down-regulation of miRNA biogenesis promotes Cbl-b expression and deactivating T cells in vitro/in vivo. CONCLUSION: Hypoxia-mediated down-regulation of miRNAs' biogenesis promotes tumor immune escape in bladder cancer, which could bring much more advance to the medical research on tumors.
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Regulación hacia Abajo/inmunología , MicroARNs/metabolismo , Escape del Tumor/inmunología , Hipoxia Tumoral/inmunología , Neoplasias de la Vejiga Urinaria/inmunología , Proteínas Adaptadoras Transductoras de Señales/genética , Animales , Apoptosis , Complejo CD3/metabolismo , Antígenos CD4/metabolismo , Antígenos CD8/metabolismo , ARN Helicasas DEAD-box/genética , Modelos Animales de Enfermedad , Regulación Neoplásica de la Expresión Génica , Humanos , Interleucina-2/metabolismo , Leucocitos Mononucleares/metabolismo , Masculino , Ratones Endogámicos C57BL , MicroARNs/biosíntesis , Estudios Prospectivos , Proto-Oncogenes Mas , Proteínas Proto-Oncogénicas c-cbl/genética , Distribución Aleatoria , Ribonucleasa III/genética , Factor de Necrosis Tumoral alfa/metabolismo , Regulación hacia Arriba , Neoplasias de la Vejiga Urinaria/metabolismoRESUMEN
AIMS: Suilysin is a critical pore-forming virulence factor of Streptococcus suis that has been demonstrated to substantially contribute to its pathogenicity. We have demonstrated that formononetin alleviates S. suis infection both in vivo and in vitro by targeting suilysin. However, the molecular mechanism of the effect is unclear. Our aim was to determine the molecular mechanism of the effect of formononetin on suilysin. METHODS AND RESULTS: The mechanism of interaction between formononetin and suilysin was investigated by molecular modelling. The results indicated that formononetin was bound at the junction of domain two and domain four of suilysin. The binding free energy values indicated that the A415, Y412, E414, N413, T61, T62 and G416 residues are critical for this binding, this observation was confirmed by the changes in the flexibility of these residues and the distances between these residues and formononetin. The inhibitory effect of formononetin on the pore-forming activity of suilysin, binding constant and binding free energy were significantly decreased by site-specific mutagenesis of Y412 and N413. Finally, we analysed the spatial configuration of suilysin before and after formononetin binding, the results indicated that the binding changed the conformation of suilysin, especially the angle between domain two and domain four, resulting in the disruption of cholesterol binding to suilysin and in the loss of pore-forming activity. CONCLUSIONS: Formononetin is located at the junction of domain two and domain four of suilysin, and Y412 and N413 play critical roles in the binding. Formononetin binding changes the angle between domain two and domain four of suilysin, resulting in the loss of the pore-inducing activity of suilysin. SIGNIFICANCE AND IMPACT OF THE STUDY: This work will promote the application of formononetin to combat S. suis infections and may contribute to the development of new inhibitors or modification of existing inhibitors.
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Infecciones Estreptocócicas , Streptococcus suis , Proteínas Hemolisinas , Humanos , IsoflavonasRESUMEN
PURPOSE: Gitelman syndrome (GS) is an autosomal recessive renal tubular disease that arises as a consequence of mutations in the SLC12A3 gene, which codes for an Na-Cl cotransporter (NCC) in distal renal tubules. This study was designed to explore the mutations associated with GS in an effort to more fully understand the molecular mechanisms governing GS. METHODS: We analyzed SLC12A3 mutations in a pedigree including a 42-year-old male with GS as well as four related family members over three generations using Sanger and next generation sequencing approaches. We additionally explored the functional ramifications of identified mutations using both Xenopus oocytes and the HEK293T cell line. RESULTS: We found that the subject with GS exhibited characteristic symptoms including sporadic thirst, fatigue, excess urination, and substantial hypokalemia and hypocalciuria, although magnesium levels were normal. Other analyzed subjects in this pedigree had normal laboratory findings and did not exhibit clear signs of GS. Sequencing analyses revealed that the GS subject exhibited a homozygous missense mutation (c.2874C > G, p.N958K) in exon 24 of SLC12A3. Both parents of this GS subject, as well as his older brother and daughter all exhibited heterozygous mutations at this same site. Functional analyses in Xenopus oocytes indicated that this mutated SLC12A3 gene encodes a protein which fails to mediate normal sodium transport, and when this mutant gene was expressed in HEK293T cells, we observed significant increases in endoplasmic reticulum (ER)-stress pathway activation. CONCLUSION: The p.N958K mutation in exon 24 of SLC12A3 can trigger GS at least in part via enhancing ER stress responses.
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Estrés del Retículo Endoplásmico , Síndrome de Gitelman/patología , Homocigoto , Mutación Missense , Adulto , Femenino , Síndrome de Gitelman/etiología , Síndrome de Gitelman/metabolismo , Humanos , Masculino , Linaje , Pronóstico , Miembro 3 de la Familia de Transportadores de Soluto 12/genéticaRESUMEN
BACKGROUND: Hand hygiene is a critical strategy for infection prevention in all healthcare settings. Automated electronic monitoring systems are expected to improve hand hygiene performance. AIM: To investigate the impact of the Internet of Things (IoT) management system on hand hygiene compliance among medical staff in an emergency intensive care unit (EICU). METHODS: This retrospective observational study was conducted between July 1st, 2017 and February 28th, 2018 in a 19-bed EICU. The changes in hand hygiene compliance among 54 members of medical staff and the incidence of hospital infections were compared, counted, and analysed before and after implementing the IoT management system in the EICU that was initiated on November 1st, 2017. FINDINGS: After the application of the IoT management system, the hand hygiene compliance rates among the members of the medical staff before (29.5% (3347/11,338) vs 57.9% (4690/8094), P < 0.001) and after (59.9% (9915/16,556) vs 73.8% (17,194/23 286), P < 0.001) the contact with patients and surrounding environment significantly improved. However, hand hygiene compliance among three cleaning staff did not significantly improve after the application. Moreover, there was no significant difference in healthcare-acquired infection rates (2.535% (9/355) vs 2.047% (7/342), P = 0.667) nor in the detection rates of the four major multidrug-resistant bacteria in the EICU before and after the application of the IoT management system (P > 0.05). CONCLUSION: The IoT management system significantly improved hand hygiene compliance among medical staff, except cleaners, in the EICU of one provincial hospital; however, the rates of nosocomial infection did not significantly decrease. The quality of hand hygiene implementation needs to be improved.
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Infección Hospitalaria , Adhesión a Directriz , Higiene de las Manos , Unidades de Cuidados Intensivos , Internet de las Cosas , Servicio de Urgencia en Hospital , Desinfección de las Manos , Humanos , Control de Infecciones , Estudios RetrospectivosAsunto(s)
Embolia , Embolia Pulmonar , Fracturas de la Columna Vertebral , Vertebroplastia , Cementos para Huesos/efectos adversos , Embolia/diagnóstico por imagen , Embolia/etiología , Humanos , Embolia Pulmonar/diagnóstico por imagen , Embolia Pulmonar/etiología , Fracturas de la Columna Vertebral/diagnóstico por imagen , Fracturas de la Columna Vertebral/etiología , Fracturas de la Columna Vertebral/cirugía , Vertebroplastia/efectos adversosAsunto(s)
Betacoronavirus , Infecciones por Coronavirus/diagnóstico por imagen , Enfermedad Crítica , Derrame Pleural/diagnóstico por imagen , Neumonía Viral/diagnóstico por imagen , COVID-19 , Femenino , Humanos , Unidades de Cuidados Intensivos , Persona de Mediana Edad , Monitoreo Fisiológico/métodos , Pandemias , SARS-CoV-2RESUMEN
The purification of parasite-infected erythrocytes from whole blood containing leucocytes is crucial for many downstream genetic and molecular assays in parasitology. Current methodologies to achieve this are often costly and time consuming. Here, we demonstrate the successful application of a cheap and simple Non-Woven Fabric (NWF) filter for the purification of parasitized red blood cells from whole blood. NWF filtration was applied to the malaria-parasitized blood of three strains of mice, and one strain of rat, and to Babesia gibsoni parasitized dog blood. Before and after filtration, the white blood cell (WBC) removal rates and red blood cell (RBC) recovery rates were measured. After NWF filter treatment of rodent malaria-infected blood, the WBC removal rates and RBC recovery rates were, for Kunming mice: 99.51%±0.30% and 86.12%±8.37%; for BALB/C mice: 99.61%±0.15% and 80.74%±7.11%; for C57 mice: 99.71%±0.12% and 84.87%±3.83%; for Sprague-Dawley rats: 99.93%±0.03% and 83.30%±2.96%. Microscopy showed WBCs were efficiently removed from infected dog blood samples, and there was no obvious morphological change of B. gibsoni parasites. NWF filters efficiently remove leukocytes from malaria parasite-infected mouse and rat blood, and are also suitable for filtration of B. gibsoni-infected dog blood.
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Babesia , Separación Celular/métodos , Eritrocitos/parasitología , Plasmodium , Animales , Perros , Femenino , Filtración , Leucocitos , Ratones , Ratones Endogámicos BALB C , Ratas Sprague-DawleyRESUMEN
BACKGROUND: The correlation between cardiovascular risk scoring systems and the severity of coronary artery diseases (CAD) is not clear. The present research aimed to evaluate the Multi-Ethnic Study of Atherosclerosis (MESA) risk score and Framingham risk score (FRS), using the Gensini score (GS) system as reference, so as to determine which model is better for the prediction of CAD severity. METHODS: This research was a single-center and cross-sectional observational study. In total, 1423 patients were included in our study. Three different groups were formed according to GS: 0â¯< GSâ¯≤ 22 (low GS group, nâ¯= 484); 22â¯< GSâ¯≤ 42 (intermediate GS group, nâ¯= 468); GSâ¯> 42 (high GS group, nâ¯= 471). Logistic and linear regression analyses were carried out to explore the relationship between the risk score models and the GS. The performance of the risk models was determined by receiver operating characteristic curve (ROC) analysis. RESULTS: The MESA risk score and the FRS both had a statistically significant power for the prediction of CAD severity (MESA area under curve: 0.630; FRS area under curve: 0.613). Furthermore, the MESA had a better performance in predicting the severity (pâ¯< 0.05) of CAD compared with the FRS. In the subgroup analysis, the MESA showed a better performance in the male (pâ¯< 0.05), diabetes mellitus (pâ¯< 0.05), and smoking subgroups (pâ¯< 0.05) compared with the FRS. CONCLUSION: The MESA and FRS predicted the severity of CAD in the Chinese population of this study. Moreover, the MESA had a better performance than the FRS model in predicting the severity of CAD in the overall population as well as in the male, smoking, diabetes, and non-diabetes subgroups.
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Aterosclerosis , Enfermedad de la Arteria Coronaria , Angiografía Coronaria , Enfermedad de la Arteria Coronaria/diagnóstico por imagen , Enfermedad de la Arteria Coronaria/epidemiología , Estudios Transversales , Humanos , Masculino , Curva ROC , Medición de Riesgo , Factores de Riesgo , Índice de Severidad de la EnfermedadRESUMEN
@#The purification of parasite-infected erythrocytes from whole blood containing leucocytes is crucial for many downstream genetic and molecular assays in parasitology. Current methodologies to achieve this are often costly and time consuming. Here, we demonstrate the successful application of a cheap and simple Non-Woven Fabric (NWF) filter for the purification of parasitized red blood cells from whole blood. NWF filtration was applied to the malaria-parasitized blood of three strains of mice, and one strain of rat, and to Babesia gibsoni parasitized dog blood. Before and after filtration, the white blood cell (WBC) removal rates and red blood cell (RBC) recovery rates were measured. After NWF filter treatment of rodent malaria-infected blood, the WBC removal rates and RBC recovery rates were, for Kunming mice: 99.51%±0.30% and 86.12%±8.37%; for BALB/C mice: 99.61%±0.15% and 80.74%±7.11%; for C57 mice: 99.71%±0.12% and 84.87%±3.83%; for Sprague-Dawley rats: 99.93%±0.03% and 83.30%±2.96%. Microscopy showed WBCs were efficiently removed from infected dog blood samples, and there was no obvious morphological change of B. gibsoni parasites. NWF filters efficiently remove leukocytes from malaria parasite-infected mouse and rat blood, and are also suitable for filtration of B. gibsoni-infected dog blood.
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Tracheophilus cymbius (Trematoda: Cyclocoelidae) is a common tracheal fluke of waterfowl, causing serious loss in the poultry industry. However, taxonomic identification of T. cymbius remains controversial and confused. Mitochondrial (mt) genomes can provide genetic markers for the identification of closely related species. We determined the mt genome of T. cymbius and reconstructed phylogenies with other trematodes. The T. cymbius mt genome is 13,760 bp in size, and contains 12 protein-coding genes (cox 1-3, nad 1-6, nad 4L, cyt b and atp 6), 22 transfer RNA (tRNA) genes, two ribosomal RNA genes and one non-coding region. All are transcribed in the same direction. The A + T content is 62.82%. ATG and TAG are the most common initiation and termination codons, respectively. Phylogenetic analyses of concatenated nucleotide sequences show T. cymbius grouping in suborder Echinostomata, and clustering together, with high statistical support, as a sister taxon with Echinochasmus japonicus (Echinochasmidae), the two forming a distinct branch rooted to the ancestor of all Echinostomatidae and Fasciolidae species. This is the first report of the T. cymbius mt genome, and the first reported mt genome within the family Cyclocoelidae. These data will provide a significant resource of molecular markers for studying the taxonomy, population genetics and systematics of trematodes.