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Mastitis is an inflammation of the mammary gland that can be caused by various factors, including biological, chemical, mechanical, or physical. Microbiological culture, DNA techniques, and high-throughput next-generation sequencing have been used to identify mastitis-causing pathogens in various animal species. However, little is known about microbiota and microbiome changes linked to yak milk mastitis. This study aimed to characterize the milk microbiota of healthy and mastitis-infected yaks using full-length 16S rRNA sequencing. The results showed that the bacterial microbiota comprises 7 phyla, 9 classes, 20 orders, 39 families, 59 genera, and 72 species. Proteobacteria and Firmicutes were the predominant microbial communities, with lower abundances of Bacteroidota, Actinobacteriota, Acidobacteriota, and other minor groupings also observed. Proteobacteria dominated the clinical and subclinical mastitis groups (95.36% and 89.32%, respectively), in contrast to the healthy group (60.17%). Conversely, Firmicutes were more common in the healthy group (39.7%) than in the subclinical and clinical mastitis groups (10.49% and 2.92%, respectively). The predominant organisms found in the healthy group were Leuconostoc mesenteroides, Lactococcus piscium, Carnobacterium maltaromaticum, and Lactococcus raffinolactis. Low abundances of Staphylococcus aureus species were found in both subclinical and clinical mastitis groups, with Moraxella osloensis and Psychrobacter cibarius dominating the subclinical mastitis group and Pseudomonas fluorescens dominating the clinical mastitis group. An alpha diversity study revealed that the healthy group had a higher microbial diversity than the clinical and subclinical mastitis groups. According to beta-diversity analysis, the principal coordinate analysis identified that mastitis-infected samples significantly differed from healthy ones. The milk microbiota of healthy yaks is more varied, and specific prominent taxa within various groups can act as marker microorganisms for mastitis risk. The genera Leuconostoc and Lactococcus are promising candidates for creating probiotics.
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(1) Background: Bangor Sewa sheep are an economically significant livestock species on the plateau. The roles of microbiota in reproduction are complex and critical for animal health. But little is known currently about the microbiome of plateau Bangor Sewa sheep. The purpose of this study was to discover the changes in the genital tract microbiota of pre- and post-partum Bangor Sewa sheep. (2) Methods: Samples from the birth canal were obtained for 16S rRNA sequencing, three days before and after delivery, respectively. (3) Results: The results showed that there was a noticeable difference in three phyla and 74 genera between the pre- and post-parturition groups in the microbiota of Bangor Sewa sheep. The changes included a decrease in the abundance of genera related to health (unclassified_Cellulomonadaceae, Cellulomonas, Fibrobacti, Flavobacterium, Eubacterium_ventriosum_group, Acetitomaculum, Aeromicrobium, Dietzia, Romboutsia, Ruminococcus, etc.) and an increased abundance of negatively related genera (Nocardioides, unclassified_Clostridia, Sphingobacteriaceae, unclassified_Ruminococcaceae, Prevotellaceae_UCG_004, Micromonospora, Streptococcus, Facklamia, Bosea, etc.) spp. (4) Conclusions: Microbes can serve as indicators of the physical state of Bangor Sewa sheep. These findings laid the foundation for deciphering the effects of microbial changes during birth on the reproductive health of plateau Bangor Sewa sheep.
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Cystic Echinococcosis (hydatidosis) is caused by the larval stage of Echinococcus spp. It is an animal-borne zoonotic parasitic disease with local epidemic and natural foci, which is very common in northwest China. In recent years, a considerable attention has been paid to the epidemic investigation of hydatid disease in humans and sheep, but there are few large-scale epidemic investigation and data analysis of bovine hydatid disease. We systematically reviewed and analyzed the prevalence of bovine hydatid disease (2000-2021) in China for the first time. Several databases including CNKI, Wanfang, VIP Chinese periodical database, Baidu Library, PubMed and ScienceDirect were used to search 57 articles and 72 sets of valid data about bovine hydatid disease in China from 2000 to 2021. We used the random effect model in META package of R software, and PAS for rate conversion. The subgroup analysis and univariate meta regression analysis were used to reveal the factors leading to the heterogeneity of the study. The total prevalence rate of bovine hydatid disease in China from 2000 to 2021 is estimated to be 17.27% (10898/63113). According to the analysis of sampling years, the lowest positive rate since 2016 is 7.54% (1503/19929). The highest prevalence rate of bovine hydatid disease is 53.93% (4340/8048). The infection rate of bovine liver accounted for the highest proportion of the total infections, 45.2% (2040/4507). We also assessed the effects of different geographical and climatic factors on the prevalence of bovine hydatid disease. The results showed that the prevalence rate of hydatid disease was higher in cold and humid areas. Although the infection rate of bovine hydatid disease has declined in recent years, it is still necessary to carry out long-term surveillance and control of hydatid disease, cut off the infection route and reduce the risk of infection in high-risk areas.
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Enfermedades de los Bovinos , Equinococosis , Epidemias , Animales , Bovinos , Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/parasitología , China/epidemiología , Equinococosis/epidemiología , Equinococosis/parasitología , Equinococosis/veterinaria , Prevalencia , OvinosRESUMEN
Lactobacillus fermentum (L. fermentum) YLF016 is a well-characterized probiotic with several favorable characteristics. This study aimed to analyze the probiotic characteristics of L. fermentum and uncover the genes implicated in its potential probiotic ability on the base of its genomics features. The complete genome of L. fermentum YLF016 was found to have a circular chromosome of 2,094,354 bp, and 51.46% G + C content without any plasmid. Its chromosome contained 2,130 predicted protein-encoding genes, 58 tRNA, and 15 rRNA-encoding genes. Also, it was found to have many other probiotic properties, such as a high survival rate in the gastrointestinal tract with strong adherence to intestinal cells, antibacterial activity against pathogens, and antioxidant activity. Moreover, the genome sequence analysis demonstrated specific genes coding for carbon metabolism pathway, genetic adaption, stress resistance, and adhesive ability. Further analysis revealed its non-hemolytic activity and its non-functional ability of virulence factors. In conclusion, L. fermentum YLF016 possesses many valuable probiotic properties that refer to its potential probiotic ability.
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Limosilactobacillus fermentum , Probióticos , Antibacterianos , Tracto Gastrointestinal , Limosilactobacillus fermentum/genéticaRESUMEN
This paper discusses compensation strategies for the aberration fields caused by the error in the radius of curvature (ROC) of the primary mirror (PM) in pupil-offset off-axis three-mirror anastigmatic (TMA) astronomical telescopes. Based on the nodal aberration theory framework, the specific astigmatic and coma aberrations of the off-axis three-mirror system in the presence of the ROC error of the PM are derived. It is demonstrated that some field-dependent aberration components can be induced by ROC error in the off-axis TMA telescopes, apart from the dominating field-constant aberration terms. To reduce the influence of the ROC error on the aberration fields, we propose two aberration compensation strategies: adjusting the position of the PM and introducing axial misalignment of the secondary mirror (SM). Through theoretical analysis and simulations, we conclude that the compensation strategy of changing the axial position of the PM can make the aberration distribution close to the nominal state; the compensation strategy of axially adjusting the SM can make the aberration distribution meet the observation requirements, which is more suitable for space applications. We also discuss compensating the effect of the ROC error using lateral misalignments.
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Space-based optical astronomical telescopes are susceptible to mirror misalignments due to space disturbance in mechanics and temperature. Therefore, it is of great importance to actively align the telescope in orbit to continuously maintain imaging quality. Traditional active alignment methods usually need additional delicate wavefront sensors and complicated operations (such as instrument calibration and pointing adjustment). This paper proposes a novel active alignment approach by matching the geometrical features of several stellar images at arbitrary multiple field positions. Based on nodal aberration theory and Fourier optics, the relationship between stellar image intensity distribution and misalignments of the system can be modeled for an arbitrary field position. On this basis, an objective function is established by matching the geometrical features of the collected multi-field stellar images and modeled multi-field stellar images, and misalignments can then be solved through nonlinear optimization. Detailed simulations and a real experiment are performed to demonstrate the effectiveness and practicality of the proposed approach. This approach eliminates the need for delicate wavefront sensors and pointing adjustment, which greatly facilitates the maintainance of imaging quality.
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This paper presents an optical alignment strategy for complex perturbed pupil-offset off-axis reflective telescopes, based on the extension of nodal aberration theory (NAT). First, the direct expansion of the wave aberration function in the vector form for perturbed off-axis systems is given, which is especially convenient for the expansion of the corresponding higher-order terms. The inherent vector relationships between the contributions generated by the aberrations of the on-axis parent systems through pupil transformation are disclosed in detail, which is helpful to understand the aberration behavior of off-axis systems. Then, according to the inherent vector relationships, an analytical alignment model based on NAT for complex cases of perturbed off-axis telescopes is established. It can quantitatively separate the effects of misalignments and surface figure errors from the total aberration fields. The alignment model is solved by using particle swarm optimization algorithm. Then, an optical alignment example of the off-axis three-mirror anastigmatic telescope with misalignments and complex surface figure errors based on the proposed method is demonstrated. After correction, the perturbed telescope can be nearly restored to the nominal states. Finally, Monte Carlo simulations are carried out to show the effectiveness and accuracy of the proposed method.
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Toxoplasma gondii is an intracellular parasite that is extensively prevalent globally. Studies have indicated the presence of T. gondii infection in animals in some provinces of China, but little is known about T. gondii infection in yaks (Bos grunniens) on the Qinghai-Tibetan Plateau. In the current study, to determine the seroprevalence and associated risk factors of T. gondii, a total of 2784 serum samples were collected from 18 different sampling sites in eight counties of the Qinghai and Tibet regions of China from 2018 to 2019. Serum antibodies against T. gondii were detected in 261 yaks (9.38%) via enzyme-linked immunosorbent assay (ELISA). We found that seroprevalence differed significantly among different counties (ranging from 5.41% in Gangcha to 19.79% in Datong), by year in the Tibet Autonomous Region (from 2.34% in 2018 to 13.24% in 2019), and by age (from 5.59% in 0 < year ≤ 1 to 11.76% in year > 7) (p < 0.05). Climate, geographical conditions, and age are the main factors influencing T. gondii infection in yaks in these regions. Therefore, our study provides a data reference for public health and prevention of yak toxoplasmosis.
TITLE: Séroprévalence et facteurs de risque associés à l'infection par Toxoplasma gondii chez les yaks (Bos grunniens) du plateau QinghaiTibet en Chine. ABSTRACT: Toxoplasma gondii est un parasite intracellulaire largement répandu dans le monde. Des études ont indiqué la présence d'une infection par T. gondii chez les animaux dans certaines provinces de Chine, mais on connaît peu l'infection par T. gondii chez les yaks (Bos grunniens) sur le plateau QinghaiTibet. Dans la présente étude, pour déterminer la séroprévalence et les facteurs de risque associés de T. gondii, un total de 2784 échantillons de sérum ont été prélevés sur 18 sites d'échantillonnage différents dans huit comtés des régions du Qinghai et du Tibet en Chine entre 2018 et 2019. Des anticorps sériques contre T. gondii ont été détectés par dosage immuno-enzymatique (ELISA) chez 261 yaks (9,38 %). Nous avons constaté que la séroprévalence différait considérablement entre les différents comtés (allant de 5,41 % à Gangcha à 19,79 % à Datong), d'une année à l'autre dans la région autonome du Tibet (de 2,34 % en 2018 à 13,24 % en 2019), et par âge (de 5,59 % pour les animaux de moins d'un an à 11,76 % pour ceux âgés de plus de 7 ans) (p < 0,05). Le climat, les conditions géographiques et l'âge sont les principaux facteurs influençant l'infection à T. gondii chez les yaks de ces régions. Par conséquent, notre étude fournit des données de référence pour la santé publique et la prévention de la toxoplasmose du yak.
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Enfermedades de los Bovinos , Toxoplasma , Animales , Bovinos , Enfermedades de los Bovinos/epidemiología , China/epidemiología , Factores de Riesgo , Estudios Seroepidemiológicos , Tibet/epidemiologíaRESUMEN
This paper presents an active compensation strategy for RMS wavefront error of perturbed off-axis telescopes in the framework of nodal aberration theory. First, the orthogonalized expression of the wave aberration function in the vector form for perturbed off-axis telescopes is derived by using RMS normalization. The orthogonalized aberration function is applied to analytically describe the RMS wavefront error in perturbed off-axis telescopes with circular apertures. Then, the system compensation model for perturbed off-axis telescopes is established. The compensation model takes the weighted square sum of the RMS wavefront errors at representative field points as the objective function, which is minimized to obtain the optimal compensation solution of off-axis systems with perturbation constraints. The compensation model is solved by using a particle swarm optimization algorithm. Then, the off-axis three-mirror anastigmatic telescope is taken as an example, and the system compensations for the misaligned tertiary mirror and deformed primary mirror are discussed. After compensation, the average RMS wavefront errors in the perturbed off-axis systems are greatly reduced, which can well meet the system requirements. Finally, Monte Carlo simulations of the optimal compensation method and sensitivity table method are carried out to demonstrate the correctness and accuracy of the proposed method.
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This paper presents a systematic and deep discussion on the aberration field characteristics of pupil-offset off-axis two-mirror astronomical telescopes induced by the radius of curvature (ROC) error based on the framework of the nodal aberration theory (NAT). The expressions of the third-order aberrations in off-axis two-mirror astronomical telescopes with ROC error are derived first. Then the astigmatic and coma aberration fields are discussed, and it is shown in a field constant astigmatism and coma will be induced by ROC error. The aberration compensation between axial misalignments and ROC error are further discussed, and it is shown that the net astigmatic and coma aberration field induced by ROC error can well be compensated by axial misalignments. Importantly, it is also demonstrated that the focal plane shift induced by ROC error can also be compensated at the same time. Also, this paper briefly analyzes the aberration field characteristics when there is the error of conic constant in optical system. Some other discussions are also presented concerning the ROC inconsistency in astronomical telescopes with a segmented primary mirror. This work will lead to a deep understanding of the influence of ROC error in pupil-offset off-axis astronomical telescopes.
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Due to the absence of rotational symmetry, the effects of axial and lateral misalignments couple tightly together, which leads to special aberration field characteristics. This paper will present an in-depth and systematic discussion on the interactions between the effects of axial and lateral misalignments in pupil-offset off-axis two-mirror astronomical telescopes. The aberration function of this class of telescopes in the presence of axial and lateral misalignments is derived. The specific expressions of two dominant non-rotationally symmetric aberrations, i.e., astigmatism and coma, are obtained and the aberration field characteristics are discussed. Importantly, it is shown that under certain conditions, a node will arise in the field of view for these two kinds of aberrations. Then the aberrational compensation mechanisms between axial and lateral misalignments are quantitatively explicated, and it is shown that the non-rotationally symmetric aberrations induced by axial misalignments can well be compensated by lateral misalignments. However, we also find that in this process, the defocus aberration induced by these two kinds of misalignments will accumulate (rather than cancel out). Therefore, in practice, it is better to separate these two kinds of misalignment. Finally, we propose a simple method to decouple axial misalignments from lateral misalignments with wavefront measurement at one field position. Most of this work can be extended to other kind of pupil-offset off-axis astronomical telescopes, such as off-axis three-mirror anastigmatic telescopes.
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Due to the absence of rotational symmetry, off-axis astronomical telescopes with off-set pupil become subject to rotational misalignments. Rotational misalignments of large off-axis mirrors with reference to their geometric center can greatly degrade the imaging quality. This paper presents an in-depth discussion on the net aberration fields of off-axis astronomical telescopes induced by rotational misalignments. Aberration function of off-axis telescopes with rotational misalignments is derived based on the framework of nodal aberration theory. Expressions of several important aberrations are obtained under some approximations. Then the specific field characteristics of these aberrations are presented and explicated. Meanwhile, we demonstrate that rotational misalignments can be converted to a kind of surface decenters; on the other hand, the effects of rotational misalignments have their special features which are different from the effects of general surface decenters. Besides, some other insightful discussions are further presented. This work is also applicable to the rotational misalignments of the off-axis segments of primary mirror in segmented mirror astronomical telescopes.
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In unobscured off-axis astronomical telescopes with an offset pupil, the effects of axial misalignments are very different from those in on-axis ones. Specifically, a series of nonrotationally symmetric aberrations with characteristic field dependence will be induced by axial misalignments. This paper takes off-axis two-mirror astronomical telescopes as an example to discuss the field characteristics of several important nonrotationally symmetric aberrations (including astigmatism, coma, and trefoil aberration) induced by axial misalignments in off-axis astronomical telescopes. The expressions of these aberrations are derived under some approximations. The accuracy of the proposed expressions is demonstrated. The specific field characteristics of these aberrations are presented and explicated. It is shown that the effects of axial misalignments bear strong similarities to the effects of the lateral misalignments in the symmetry plane of the off-axis system. On the other hand, the inherent relationships between astigmatism and coma induced by axial misalignments are further revealed, which are different from those induced by lateral misalignments. This fact presents the possibility of separating the effects of axial misalignments and lateral misalignments. Most of this work can be extended to other off-axis astronomical telescopes with more freedom.
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A feature-based phase retrieval wavefront sensing approach using machine learning is proposed in contrast to the conventional intensity-based approaches. Specifically, the Tchebichef moments which are orthogonal in the discrete domain of the image coordinate space are introduced to represent the features of the point spread functions (PSFs) at the in-focus and defocus image planes. The back-propagation artificial neural network, which is one of most wide applied machine learning tool, is utilized to establish the nonlinear mapping between the Tchebichef moment features and the corresponding aberration coefficients of the optical system. The Tchebichef moments can effectively characterize the intensity distribution of the PSFs. Once well trained, the neural network can directly output the aberration coefficients of the optical system to a good precision with these image features serving as the input. Adequate experiments are implemented to demonstrate the effectiveness and accuracy of proposed approach. This work presents a feasible and easy-implemented way to improve the efficiency and robustness of the phase retrieval wavefront sensing.
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In this paper, the Zernike coefficient is analytically expressed as the product of the dependence of aberration field decenter vectors (related with perturbations) and the dependence of fields of view (FOVs), on the frame work of nodal aberration theory (NAT). By expanding and analyzing this expression, an alignment strategy by optical compensation for the perturbed on-axis or off-axis telescope is presented. Specifically, two cases, corresponding to the misalignment of tertiary mirror (TM) and the deformation of primary mirror (PM), respectively, are discussed for the same three mirror anastigmatic (TMA) telescope. Here the misaligned TM and the deformed PM are compensated only by aligning secondary mirror (SM). By analyzing the aberration field after compensation with the nominal, it is found that either PM or TM can be compensated by SM. It is also found TM is more easily compensated than PM. In the end, the NAT method developed here used for optical compensation is compared to merit function regression (MFR) method and sensitivity table method (STM). By comparing NAT method with MFR method, it is shown that the calculated correction values of SM based on NAT method is very close to the referred values obtained from MFR method. It proves the correctness of NAT method developed here. By comparing NAT method with STM, it demonstrates that the computation accuracy of NAT method is much higher in poor conditions and NAT method is less sensitive to measurement errors. It is further illustrated that the theory of optical compensation by SM developed here is correct and applicable.
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This paper presents a systematic and in-depth discussion for the aberration fields of off-axis two-mirror astronomical telescopes with an offset pupil that is induced by lateral misalignment. Based on the framework of nodal aberration theory and a system level pupil coordinate transformation, the aberration function for misaligned off-axis telescopes is derived. Some general descriptions for the misalignment-induced aberrations are presented. The specific astigmatic and coma aberration field characteristics in off-axis two-mirror telescopes are then discussed. The precision of the presented aberration expressions is demonstrated. The discrepancies between the ray tracing data and aberration expressions are explicated. Then the inherent relationships between the astigmatism and coma aberration fields are revealed and explicated. Based on this knowledge, some quantitative discussions are further presented for determining the misalignments used to compensate for the effects of primary mirror astigmatic figure errors as well as separating these two effects when coupled. Other effects of lateral misalignments are also presented, especially the field-constant focal shift, which is only sensitive to the lateral misalignments in the symmetry plane of the nominal off-axis system. A quantitative discussion is also presented which explains the reason why trefoil aberration in off-axis telescopes is more sensitive to lateral misalignments. Most of the results presented in this paper can be extended to the other off-axis astronomical telescopes with more freedoms.
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Our paper mainly separates the specific aberration contributions of third-order astigmatism and third-order coma from the total aberration fields, on the framework of the modified nodal aberration theory (NAT), for the perturbed off-axis telescope. Based on the derived aberration functions, two alignment models for the same off-axis two-mirror telescope are established and compared. Among them, one is based on third-order NAT, the other is based on fifth-order NAT. By comparison, it is found that the calculated perturbations based on fifth-order NAT are more accurate. It illustrates that third-order astigmatism and third-order coma contributed from fifth-order aberrations can't be neglected in the alignment process. Then the fifth-order NAT is used for the alignment of off-axis three-mirror telescopes. After simulation, it is found that the perturbed off-axis three-mirror telescope can be perfectly aligned as well. To further demonstrate the application of the alignment method based on fifth-order NAT (simplified as NAT method), Monte-Carlo simulations for both off-axis two-mirror telescope and off-axis three-mirror telescope are conducted in the end. Meantime, a comparison between NAT method and sensitivity table method is also conducted. It is proven that the computation accuracy of NAT method is much higher, especially in poor conditions.
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In active optics systems, one concern is how to quantitatively separate the effects of astigmatic and trefoil figure errors and misalignments that couple together in determining the total aberration fields when wavefront measurements are available at only a few field points. In this paper, we first quantitatively describe the impact of mount-induced trefoil deformation on the net aberration fields by proposing a modified theoretical formulation for the field-dependent aberration behavior of freeform surfaces based on the framework of nodal aberration theory. This formulation explicitly expresses the quantitative relationships between the magnitude of freeform surfaces and the induced aberration components where the freeform surfaces can be located away from the aperture stop and decentered from the optical axis. On this basis, and in combination with the mathematical presentation of nodal aberration theory for the effects of misalignments, we present the analytic expressions for the aberration fields of two-mirror telescopes in the presence of astigmatic primary mirror figure errors, mount-induced trefoil deformations on both mirrors, and misalignments. We quantitatively separate these effects using the analytical expressions with wavefront measurements at a few field points and pointing errors. Valuable insights are provided on how to separate these coupled effects in the computation process. Monte Carlo simulations are conducted to demonstrate the correctness and accuracy of the analytic method presented in this paper.
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We present the design of a horizontally staggered lightguide solar concentrator with lateral displacement tracking for high concentration applications. This solar concentrator consists of an array of telecentric primary concentrators, a horizontally staggered lightguide layer, and a vertically tapered lightguide layer. The primary concentrator is realized by two plano-aspheric lenses with lateral movement and maintains a high F-number over an angle range of ±23.5°. The results of the simulations show that the solar concentrator achieves a high concentration ratio of 500× with ±0.5° of acceptance angle by a single-axis tracker and dual lateral translation stages.
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An off-axis three-mirror system (OTS) was designed based on the primary mirror and tertiary mirror (TM) integrated on a single substrate in order to solve the OTS drawbacks, such as the alignment difficulty and the large opto-mechanical weight. Furthermore, an optical freeform surface that can increase the optimizing degrees of freedom (DOF) was applied on the TM in order to achieve a wide field of view (FOV). An example with a focal length of 1200 mm, F-number of 12, and FOV of 10°×4° was given, and the maximum wave front error (WFE) RMS was 0.0126λ, indicating a good imaging quality. The design result shows that the number of alignment DOF was reduced from 12 to 6, and the weight of the mirror support assembly can also be lighter. An XY polynomial, established as an even function of x, was employed as the TM surface, so we obtained an axial symmetrical imaging quality about the x axis, and the axial symmetry aberration performance also brings considerable convenience to alignment and testing for the OTS.