Small biochar addition enhanced anammox granular sludge system for practical wastewater treatment: Performance and microbial community.

Anaerobic ammonium oxidation (Anammox) granular sludge (AnGS) has poor strength and is prone to disintegration under complex environmental conditions, especially in the presence of complex organic carbon, which renders the Anammox process instable. Herein, with a mixture of landfill leachate and domestic sewage as wastewater, the effect on the properties of AnGS with two small particle size (0.1-0.2 mm) biochars (coconut and peach biochars) addition were investigated at different COD concentrations (150 mg·L-1, 200 mg·L-1, and 250 mg·L-1), as well as at different BOD/TN (B/N) (0.3 and 0.5). Results showed that the nitrogen removal efficiencies decreased from 89 % to 72 % as the COD concentration increased by 100 mg·L-1, while peach biochar reactor had better nitrogen removal performance. Excessive organic carbon supply inhibits AnAOB proliferation and B/N had the most significant effect on AnAOB (p < 0.05). The Polymerase Chain Reaction (PCR) indicated peach biochar reactor get higher activity of anammox-related functional genes (hzsA, hdh).

Bamboo charcoal addition enhanced the nitrogen removal of anammox granular sludge with COD: Performance, physicochemical characteristics and microbial community.

The effects of different chemical oxygen demand (COD) concentrations on the anammox granular sludge with Bamboo Charcoal (BC) addition were evaluated in UASB reactor. The results showed that the average total nitrogen (TN) removal efficiency was reduced from 85.9% to 81.4% when COD concentration was increased from 50 to 150 mg/L. However, the TN removal efficiency of BC addition reactors was dramatically 3.1%-6.4% higher than that without BC under different COD concentrations. The average diameter of granular sludge was 0.13 mm higher than that without BC. The settling velocity was increased by elevated COD concentration, while the EPS and VSS/SS were increased with BC addition. The high-throughput Miseq sequencing analyses revealed that the bacterial diversity and richness were decreased under COD addition, and the Planctomycetes related to anammox bacteria were Candidatus Brocadia and Candidatus Kuenenia. The Metagenomic sequencing indicated that the abundance of denitrification related functional genes all increased with elevated COD, while the abundance of anammox related functional genes of decreased. The functional genes related to anammox was hydrazine synthase encoding genes (hzsA, hzsB and hzsB). The average relative abundance of hzs genes in the reactor with BC addition was higher than the control at COD concentrations of 50 mg/L and 150 mg/L. The functional genes of denitrification mediated by BC were higher than those without BC throughout the operation phase. It is interesting to note that BC addition greatly enriched the related functional genes of denitrification and anammox.