RESUMEN
A Giardia lamblia antigen detected by the TechLab Giardia Test (TechLab, Inc., Blacksburg, Va.) and the Alexon ProSpecT Giardia microplate assay (Alexon, Inc., Sunnyvale, Calif.) was purified by immunoaffinity chromatography from supernatant fluids of encystment cultures. Two major proteins (Mr 22,000 and 26,000) were observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Coomassie staining that did not resemble the GSA65 antigen reportedly detected by the Alexon test. These proteins reacted intensely with the monoclonal antibodies used in both commercial enzyme-linked immunosorbent assays (ELISAs). Both proteins had identical N-terminal amino acid sequences and were identified as cyst wall protein 1 (CWP1). The 26-kDa form appeared early during encystment followed by the appearance of the 22-kDa form. Recombinant CWP1 (Mr 26,000) was strongly positive in both commercial tests. CWP1 was stable in human stool specimens, resistant to degradation by proteases and N- and O-glycanases, and unaffected by oxidation with sodium periodate. Two minor proteins with Mrs of 32,000 and 39,000 were detected in CWP1 preparations by using a sensitive fluorescent protein stain. Both were identified as CWP2, and neither reacted with the monoclonal antibodies from the commercial tests. We analyzed 535 stool specimens for CWP1 by using both commercial ELISAs and resolved discrepant results by using routine ova and parasite examination (O&P) and on immunofluorescence antibody assay. The presence of CWP1 correlated well between both ELISAs (98.7% correlation). Our results demonstrate that both commercial ELISAs detect CWP1, which is a useful diagnostic marker because it is highly stable, is secreted in large amounts by encysting trophozoites, and correlates well with O&P.
Asunto(s)
Giardia/aislamiento & purificación , Giardiasis/diagnóstico , Proteínas Protozoarias/análisis , Animales , Antígenos de Protozoos/análisis , Western Blotting , Cromatografía de Afinidad , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción Enzimática/métodos , Humanos , Peso Molecular , Valor Predictivo de las Pruebas , Juego de Reactivos para Diagnóstico , Proteínas Recombinantes/análisis , Reproducibilidad de los ResultadosRESUMEN
We compared the E test (AB Biodisk North America, Inc., Culver City, Calif.) with the National Committee for Clinical Laboratory Standards broth microdilution method for the determination of MICs of penicillin and cefotaxime for 108 isolates of Streptococcus pneumoniae. The E test was performed following manufacturer's recommendations with Mueller-Hinton blood agar, and the broth microdilution procedure was performed with lysed horse blood-supplemented Mueller-Hinton broth. The microdilution method classified 26 isolates as highly penicillin resistant (MIC, > or = 2 micrograms/ml), 33 as intermediately resistant to penicillin (MIC, > or = 0.1 < 2.0 micrograms/ml), and 49 as susceptible to penicillin (MIC, < 0.1 micrograms/ml). Discordant results obtained with the E test for penicillin susceptibility testing compared with broth microdilution occurred for 19 of the 108 isolates tested. Cefotaxime MICs for 90% of isolates found highly resistant, intermediately resistant, and susceptible to penicillin by broth microdilution were 2.0, 0.5, and 0.06 micrograms/ml, respectively. There were 16 susceptibility category changes when the E test was used to determine cefotaxime MICs. All of the discrepancies in the penicillin and cefotaxime MICs determined by the E test occurred at the susceptibility category breakpoints, and all represented differences of only one twofold dilution factor. Properly performed and controlled, the E test should be a reliable quantitative procedure for more accurately predicting the susceptibility of S. pneumoniae to several antibiotics.
Asunto(s)
Pruebas de Sensibilidad Microbiana/métodos , Streptococcus pneumoniae/efectos de los fármacos , Cefotaxima/farmacología , Farmacorresistencia Microbiana , Estudios de Evaluación como Asunto , Humanos , Pruebas de Sensibilidad Microbiana/estadística & datos numéricos , Resistencia a las Penicilinas , Infecciones Neumocócicas/tratamiento farmacológico , Infecciones Neumocócicas/microbiología , Sensibilidad y Especificidad , Streptococcus pneumoniae/aislamiento & purificaciónRESUMEN
The role of the outer membrane and lipopolysaccharide (LPS) in the interaction between the small cationic antimicrobial peptide magainin 2 and the Gram-negative cell envelope was studied by FT-IR spectroscopy. Magainin 2 alters the thermotropic properties of the outer membrane-peptidoglycan complexes from wild-type Salmonella typhimurium and a series of LPS mutants which display differential susceptibility to the bactericidal activity of cationic antibiotics. These results are correlated with the LPS phosphorylation pattern and charge (characterized by high-resolution 31P NMR) and outer membrane lipid composition, and are compared to the bactericidal susceptibility. LPS mutants show a progressive loss of resistance to killing by magainin 2 as the length of the LPS polysaccharide moiety decreases. Disordering of the outer membrane lipid fatty acyl chains by magainin 2, however, depends primarily upon the magnitude of LPS charge rather than the length of the LPS polysaccharide, contradicting the proposal by Weiss et al. [Weiss, J., Beckerdite-Quagiata, S., & Elsbach, P. (1980) J. Clin. Invest. 65, 619-628] that the sugar side chain of LPS shields the negative charges of the outer membrane surface. While disruption of outer membrane structure most likely is not the primary factor leading to cell death, the susceptibility of Gram-negative cells to magainin 2 is associated with factors that facilitate the transport of the peptide across the outer membrane, such as the magnitude and location of LPS charge, the concentration of LPS in the outer membrane, outer membrane molecular architecture, and the presence or absence of the O-antigen side chain.
Asunto(s)
Antibacterianos/farmacología , Péptidos Catiónicos Antimicrobianos , Membrana Celular/efectos de los fármacos , Lipopolisacáridos/química , Péptidos/farmacología , Salmonella typhimurium/efectos de los fármacos , Proteínas de Xenopus , Conformación de Carbohidratos , Secuencia de Carbohidratos , Membrana Celular/química , Lipopolisacáridos/genética , Magaininas , Espectroscopía de Resonancia Magnética/métodos , Lípidos de la Membrana/análisis , Datos de Secuencia Molecular , Mutación , Peptidoglicano/química , Peptidoglicano/genética , Fosfolípidos/análisis , Salmonella typhimurium/genética , Salmonella typhimurium/crecimiento & desarrollo , TermodinámicaRESUMEN
Salmonella typhimurium and a series of rough lipopolysaccharide mutants derived from it were used as target bacteria to examine the antimicrobial capacity of magainin 2. Magainin 2 demonstrated a dose-related bactericidal activity against the smooth parent strain and the series of lipopolysaccharide mutants. The lipopolysaccharide mutant series showed an ordered increase in sensitivity to the magainin 2 as the depth of the rough lesion in the lipopolysaccharide increased.
Asunto(s)
Antibacterianos/farmacología , Péptidos Catiónicos Antimicrobianos , Péptidos/farmacología , Salmonella typhimurium/efectos de los fármacos , Proteínas de Xenopus , Secuencia de Carbohidratos , Membrana Celular/química , Membrana Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Lipopolisacáridos/genética , Magaininas , Datos de Secuencia Molecular , Salmonella typhimurium/genéticaRESUMEN
Administration of AMP soon after inoculation of mice with herpes simplex virus type 1 inhibited development of virus-induced lesions and appeared to prevent establishment of virus latency. These effects were dependent on both the AMP dose and the time of AMP administration. Regression of herpes simplex virus type 1-induced lesions was also accelerated significantly by AMP treatment in a time- and dose-dependent manner.