RESUMEN
PACAP is a neuropeptide with diverse functions in various organs, including reproductive system. It is present in the testis in high concentrations, and in addition to the stage-specific expression within the seminiferous tubules, PACAP affects spermatogenesis and the functions of Leydig and Sertoli cells. Mice lacking endogenous PACAP show reduced fertility, but the possibility of abnormalities in spermatogenic signaling has not yet been investigated. Therefore, we performed a detailed morphological analysis of spermatozoa, sperm motility and investigated signaling pathways that play a role during spermatogenesis in knockout mice. No significant alterations were found in testicular morphology or motility of sperm in homozygous and heterozygous PACAP-deficient mice in spite of the moderately increased number of severely damaged sperms. However, we found robust changes in mRNA and/or protein expression of several factors that play an important role in spermatogenesis. Protein kinase A expression was markedly reduced, while downstream phospho-ERK and p38 were elevated in knockout animals. Expression of major transcription factors, such as Sox9 and phospho-Sox9, was decreased, while that of Sox10, as a redundant factor, was increased in PACAP-deficient mice. The reduced phospho-Sox9 expression was partly due to increased expression and activity of phosphatase PP2A in knockout mice. Targets of Sox transcription factors, such as collagen type IV, were reduced in knockout mice. In summary, our results show that lack of PACAP leads to disturbed signaling in spermatogenesis, which could be a factor responsible for reduced fertility in PACAP knockout mice, and further support the role of PACAP in reproduction.
Asunto(s)
Biomarcadores/metabolismo , Polipéptido Hipofisario Activador de la Adenilato-Ciclasa/fisiología , Túbulos Seminíferos/patología , Motilidad Espermática/fisiología , Espermatogénesis , Espermatozoides/patología , Animales , Masculino , Ratones , Ratones Noqueados , Proteína Fosfatasa 2/metabolismo , Reproducción , Túbulos Seminíferos/metabolismo , Espermatozoides/metabolismoRESUMEN
Osteoblasts, the bone forming cells, affect self-renewal and expansion of hematopoietic stem cells (HSCs), as well as homing of healthy hematopoietic cells and tumor cells into the bone marrow. Constitutive activation of ß-catenin in osteoblasts is sufficient to alter the differentiation potential of myeloid and lymphoid progenitors and to initiate the development of acute myeloid leukemia (AML) in mice. We show here that Notch1 is the receptor mediating the leukemogenic properties of osteoblast-activated ß-catenin in HSCs. Moreover, using cell-specific gene inactivation mouse models, we show that FoxO1 expression in osteoblasts is required for and mediates the leukemogenic properties of ß-catenin. At the molecular level, FoxO1 interacts with ß-catenin in osteoblasts to induce expression of the Notch ligand, Jagged-1. Subsequent activation of Notch signaling in long-term repopulating HSC progenitors induces the leukemogenic transformation of HSCs and ultimately leads to the development of AML. These findings identify FoxO1 expressed in osteoblasts as a factor affecting hematopoiesis and provide a molecular mechanism whereby the FoxO1/activated ß-catenin interaction results in AML. These observations support the notion that the bone marrow niche is an instigator of leukemia and raise the prospect that FoxO1 oncogenic properties may occur in other tissues.
Asunto(s)
Factores de Transcripción Forkhead/fisiología , Leucemia Mieloide Aguda/etiología , Osteoblastos/fisiología , beta Catenina/fisiología , Anemia/etiología , Animales , Proteínas de Unión al Calcio/genética , Proteína Forkhead Box O1 , Células Madre Hematopoyéticas/fisiología , Péptidos y Proteínas de Señalización Intercelular/genética , Proteína Jagged-1 , Proteínas de la Membrana/genética , Ratones , Receptores Notch/fisiología , Proteínas Serrate-Jagged , Transducción de SeñalRESUMEN
Reduced central noradrenergic function has been implicated as a factor in reduced behavioral activity after stress. The present studies examined the role of reduced beta adrenergic neurotransmission in mediating this effect. This was done by testing the ability of beta receptor antagonists to mimic the behavioral actions of stress. Mice were subjected to stress or given various beta antagonists and tested for swimming behavior, locomotor activity, or grooming behavior. As previously reported, stress reduced swimming and locomotor activity and increased grooming. Both the nonselective antagonist, l-propranolol, and the beta-1 selective antagonist, betaxolol, produced the same effects as stress on all three measures. A beta-2 selective antagonist, ICI 118,551, was effective only on swimming, whereas a membrane stabilizing agent, d-propranolol, was effective only on grooming behavior. The peripherally active beta-1 antagonist, atenolol, was not effective on any measure. The nonspecific dopaminergic receptor blocker, fluphenazine, reduced locomotion but tended also to reduce grooming. The results indicate that blockade of beta-1 receptors in the CNS selectively mimics the action of stress on gross motor activity in mice and, along with previous data, suggest that stress leads to a relative deficiency in central beta-1 noradrenergic neurotransmission in these animals.