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Virus Genes ; 46(3): 546-50, 2013 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-23329009

RESUMEN

The feline immunodeficiency virus (FIV) is a retrovirus that is found worldwide, and it can be assigned to six subtypes (A, B, C, D, E, and a putative subtype F) based on sequencing analysis of the env and gag genes. Subtypes A and B are the most common worldwide. In Brazil, several authors have isolated only subtype B, and its prevalence differs markedly among investigated populations. Blood samples from 200 domestic felines from the Federal District in Brazil were analyzed by PCR. Samples that tested positive for FIV were then cloned, sequenced, and analyzed phylogenetically and statistically. The results represent the first description of FIV infection in the Central Region of Brazil and suggest that only 2 % of felines in this region are positive for the virus. In addition, the analysis showed that one out of the four positive samples that we detected could not be assigned to any of the six classical subtypes. This sample was taken as a putative novel subtype of the FIV virus. The remaining three positive samples were assigned to subtype B, with differences existing among these samples.


Asunto(s)
Enfermedades de los Gatos/virología , Virus de la Inmunodeficiencia Felina/clasificación , Virus de la Inmunodeficiencia Felina/aislamiento & purificación , Infecciones por Lentivirus/veterinaria , Animales , Sangre/virología , Brasil , Gatos , Clonación Molecular , Análisis por Conglomerados , Genotipo , Infecciones por Lentivirus/virología , Filogenia , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN , Homología de Secuencia
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