RESUMEN
Initiation of DNA replication at oriC occurs bidirectionally both in vivo and in vitro. Although the proteins involved in establishing the replication forks are known, little is known about the events that ensure that initiation is bidirectional. We show here that in the absence of DNA gyrase, replication fork progression from oriC on a plasmid template in vitro is unidirectional, although both replication forks have formed at the origin. There was no bias in the release of one fork or the other, ruling out protein blockage of one fork as a possible reason for the asymmetric release. Timely release of both forks required the presence of either DNA gyrase or topoisomerase IV, suggesting that modulation of the topology of the origin region is the governing factor.
Asunto(s)
Proteínas Bacterianas , Replicación del ADN/genética , Replicación del ADN/fisiología , Western Blotting , Reactivos de Enlaces Cruzados/farmacología , Girasa de ADN/metabolismo , ADN Helicasas/metabolismo , AdnB Helicasas , Escherichia coli/enzimología , Escherichia coli/genética , Cinética , Modelos Genéticos , Unión Proteica , Factores de TiempoRESUMEN
Replication forks formed at bacterial origins often encounter template roadblocks in the form of DNA adducts and frozen protein-DNA complexes, leading to replication-fork stalling and inactivation. Subsequent correction of the corrupting template lesion and origin-independent assembly of a new replisome therefore are required for survival of the bacterium. A number of models for replication-fork restart under these conditions posit that nascent strand regression at the stalled fork generates a Holliday junction that is a substrate for subsequent processing by recombination and repair enzymes. We show here that early replication intermediates containing replication forks stalled in vitro by the accumulation of excess positive supercoils could be cleaved by the Holliday junction resolvases RusA and RuvC. Cleavage by RusA was inhibited by the presence of RuvA and was stimulated by RecG, confirming the presence of Holliday junctions in the replication intermediate and supporting the previous proposal that RecG could catalyze nascent strand regression at stalled replication forks. Furthermore, RecG promoted Holliday junction formation when replication intermediates in which the replisome had been inactivated were negatively supercoiled, suggesting that under intracellular conditions, the action of RecG, or helicases with similar activities, is necessary for the catalysis of nascent strand regression.
Asunto(s)
Proteínas Bacterianas/metabolismo , ADN Helicasas/metabolismo , Replicación del ADN , ADN Bacteriano/biosíntesis , ADN Superhelicoidal/biosíntesis , Proteínas de Escherichia coli , Escherichia coli/genética , Escherichia coli/metabolismoRESUMEN
There are two modes of DNA synthesis at a replication fork. The leading strand is synthesized in a continuous fashion in lengths that in Escherichia coli can be in excess of 2 megabases. On the other hand, the lagging strand is synthesized in relatively short stretches of 2 kilobases. Nevertheless, identical assemblies of the DNA polymerase III core tethered to the beta sliding clamp account for both modes of DNA synthesis. Yet the same lagging strand polymerase accounts for the synthesis of all Okazaki fragments at a replication fork, cycling repeatedly every 1 or 2 s from the 3'-end of the just-completed fragment to the 3'-end of the new primer. Several models have been invoked to account for the rapid cycling of a polymerase complex that can remain bound to the template for upward of 40 min. By using isolated replication protein-DNA template complexes, we have tested these models and show here that cycling of the lagging strand polymerase can be triggered by either the action of primase binding to the replisome and synthesizing a primer or by collision of the lagging strand polymerase with the 5'-end of the previous Okazaki fragment.
Asunto(s)
ADN Polimerasa III/metabolismo , Replicación del ADN , Secuencia de Bases , Cartilla de ADNRESUMEN
The fundamental activities of the replicative primosomes of Escherichia coli are provided by DnaB, the replication fork DNA helicase, and DnaG, the Okazaki fragment primase. As we have demonstrated previously, DnaG is recruited to the replication fork via a transient protein-protein interaction with DnaB. Here, using site-directed amino acid mutagenesis, we have defined the region on DnaB required for this protein-protein interaction. Mutations in this region of DnaB affect the DnaB-DnaG interaction during both general priming-directed and phiX174 complementary strand DNA synthesis, as well as at replication forks reconstituted in rolling circle DNA replication reactions. The behavior of the purified mutant DnaB proteins in the various replication systems suggests that access to the DnaG binding pocket on DnaB may be restricted at the replication fork.
Asunto(s)
Proteínas Bacterianas , ADN Helicasas/metabolismo , ADN Primasa/metabolismo , ADN/metabolismo , Sustitución de Aminoácidos , Bacteriófago phi X 174/genética , ADN Helicasas/genética , Replicación del ADN , ADN Viral/metabolismo , AdnB Helicasas , Electroforesis en Gel de Poliacrilamida , Escherichia coli , Modelos Moleculares , Relación Estructura-ActividadRESUMEN
The encounter of a replication fork with either a damaged DNA template, a nick in the template strand or a 'frozen' protein-DNA complex can stall the replisome and cause it to fall apart. Such an event generates a requirement for replication fork restart if the cell is going to survive. Recent evidence shows that replication fork restart is effected by the action of the recombination proteins generating a substrate for PriA-directed replication fork assembly.
Asunto(s)
Replicación del ADN/fisiología , Proteínas de Unión al ADN/fisiología , Escherichia coli/genética , Cromosomas Bacterianos , Fenotipo , Proteína de Replicación ARESUMEN
A contact between the dimeric tau subunit within the DNA polymerase III holoenzyme and the DnaB helicase is required for replication fork propagation at physiologically-relevant rates (Kim, S., Dallmann, H. G., McHenry, C. S., and Marians, K. J. (1996) Cell 84, 643-650). In this report, we exploit the OmpT protease to generate C-tau, a protein containing only the unique C-terminal sequences of tau, free of the sequences shared with the alternative gamma frameshifting product of dnaX. We have established that C-tau is a monomer by sedimentation equilibrium and sedimentation velocity ultracentrifugation. Monomeric C-tau binds the alpha catalytic subunit of DNA polymerase III with a 1:1 stoichiometry. C-tau also binds DnaB, revealed by a coupled immunoblotting method. C-tau restores the rapid replication rate of inefficient forks reconstituted with only the gamma dnaX gene product. The acceleration of the DnaB helicase can be observed in the absence of primase, when only leading-strand replication occurs. This indicates that C-tau, bound only to the leading-strand polymerase, can trigger the conformational change necessary for DnaB to assume the fast, physiologically relevant form.
Asunto(s)
Proteínas Bacterianas/química , Proteínas Bacterianas/metabolismo , ADN Helicasas/química , ADN Helicasas/metabolismo , ADN Polimerasa III/química , ADN Polimerasa III/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas Bacterianas/aislamiento & purificación , ADN Helicasas/aislamiento & purificación , ADN Polimerasa III/aislamiento & purificación , Dimerización , AdnB Helicasas , Cinética , Sustancias Macromoleculares , Peso Molecular , Estructura Cuaternaria de Proteína , Origen de RéplicaRESUMEN
A bacterial housekeeping function, which requires both recombination and replication enzymes, has been identified that re-establishes inactivated replication forks under normal growth conditions. Some long-tract gene-conversion events initiated by double-strand breaks in yeast and mammalian cells can be attributed to recombination-directed DNA replication. Double-strand break repair in yeast has been shown to require both leading- and lagging-strand DNA synthesis. These observations suggest that the recombination and replication machinery cooperate to maintain genomic integrity.
Asunto(s)
Replicación del ADN/genética , Recombinación Genética , Animales , ADN Bacteriano/genética , HumanosRESUMEN
The bacterial SOS response to unusual levels of DNA damage has been recognized and studied for several decades. Pathways for re-establishing inactivated replication forks under normal growth conditions have received far less attention. In bacteria growing aerobically in the absence of SOS-inducing conditions, many replication forks encounter DNA damage, leading to inactivation. The pathways for fork reactivation involve the homologous recombination systems, are nonmutagenic, and integrate almost every aspect of DNA metabolism. On a frequency-of-use basis, these pathways represent the main function of bacterial DNA recombination systems, as well as the main function of a number of other enzymatic systems that are associated with replication and site-specific recombination.
Asunto(s)
Bacterias/genética , Replicación del ADN , Respuesta SOS en Genética , Proteínas Bacterianas/fisiología , Cromosomas Bacterianos , ADN Bacteriano/genética , Escherichia coli/genética , Recombinación Genética , Origen de RéplicaRESUMEN
Escherichia coli strains lacking PriA are severely compromised in their ability to repair UV-damaged DNA and to perform homologous recombination. These phenotypes arise because of a lack of PriA-directed replication fork assembly at recombination intermediates such as D-loops. Naturally arising suppressor mutations in dnaC restore strains carrying the priA2::kan null allele to wild-type function. We have cloned one such gene, dnaC810, and overexpressed, purified, and characterized the DnaC810 protein. DnaC810 can support a PriA-independent synthesis of phiX174 complementary strand DNA. This can be attributed to its ability, unlike wild-type DnaC, to catalyze a SSB-insensitive general priming reaction with DnaB and DnaG on any SSB-coated single-stranded DNA. Gel mobility shift analysis revealed that DnaC810 could load DnaB directly to SSB-coated single-stranded DNA as well as to D loop DNA. This explains the ability of DnaC810 to bypass the requirement for PriA, PriB, PriC, and DnaT during replication fork assembly at recombination intermediates.
Asunto(s)
Proteínas Bacterianas/genética , Bacteriófago phi X 174/genética , ADN Helicasas/metabolismo , Replicación del ADN , Proteínas de Unión al ADN/metabolismo , Proteínas de Escherichia coli , Mutación , Proteínas Bacterianas/aislamiento & purificación , Proteínas Bacterianas/metabolismo , ADN de Cadena Simple/metabolismo , AdnB Helicasas , Escherichia coli/virología , Unión Proteica , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Proteína de Replicación A , Supresión GenéticaRESUMEN
A 17-amino-acid residue domain has been identified in Escherichia coli DNA topoisomerase III (Topo III) that is essential for Topo III-mediated resolution of DNA replication intermediates in vitro. Deletion of this domain reduced Topo III-catalysed resolution of DNA replication intermediates and decatenation of multiply linked plasmid DNA dimers by four orders of magnitude, whereas reducing Topo III-catalysed relaxation of negatively supercoiled DNA substrates only 20-fold. The presence of this domain has been detected in multiple plasmid-encoded topoisomerases, raising the possibility that these enzymes may also be decatenases.
Asunto(s)
ADN-Topoisomerasas de Tipo I/metabolismo , Escherichia coli/enzimología , Secuencia de Aminoácidos , Sitios de Unión , Replicación del ADN , ADN-Topoisomerasas de Tipo I/química , ADN-Topoisomerasas de Tipo I/genética , ADN Bacteriano/genética , ADN Bacteriano/metabolismo , ADN Superhelicoidal/química , ADN Superhelicoidal/metabolismo , Dimerización , Escherichia coli/genética , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Unión Proteica , Estructura Terciaria de Proteína , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genética , Homología de Secuencia de Aminoácido , Especificidad por SustratoRESUMEN
ParE is the ATP-binding subunit of topoisomerase IV (Topo IV). During topoisomerization, the ATP-binding and hydrolysis cycle must be coordinated with the cycle of DNA cleavage and religation. We have isolated three dominant-negative mutant alleles of parE that encode ParE proteins that fail to hydrolyze ATP when reconstituted with ParC to form Topo IV. ParE G110S Topo IV and ParE S123L Topo IV failed to bind ATP at all, whereas ParE T201A could bind ATP. All three mutant Topo IV proteins exhibited an elevated level of spontaneous DNA cleavage that could be associated with a decreased rate of DNA resealing. In ParE T201A Topo IV, this defect appeared to result from an increased likelihood that the tetrameric enzyme would fall apart after DNA cleavage. Thus, while ATP is not required for DNA cleavage, the properties of these mutant enzymes suggests that ATP-hydrolysis informs DNA religation.
Asunto(s)
Adenosina Trifosfatasas/genética , Proteínas Bacterianas/genética , ADN-Topoisomerasas de Tipo II/genética , Proteínas de Unión al ADN/genética , Escherichia coli/enzimología , Adenosina Trifosfato/metabolismo , Adenilil Imidodifosfato/metabolismo , Animales , Cromatografía en Gel , Crithidia fasciculata , Análisis Mutacional de ADN , Topoisomerasa de ADN IV , ADN Superhelicoidal/metabolismo , Dimerización , Modelos Moleculares , MutaciónRESUMEN
In order to define regions of ParE, one of the two subunits of topoisomerase IV, that are involved in catalysis during topoisomerization, we developed a selection procedure to isolate dominant-negative parE alleles. Both wild-type parC and mutagenized parE were expressed from a tightly-regulated lac promoter on a moderate-copy plasmid. Mutated parE alleles were rescued from those plasmids that caused IPTG-dependent cell death. The mutant ParE proteins could be divided into two groups when reconstituted with ParC to form topoisomerase IV, those that elicited hyper-DNA cleavage and those that affected covalent complex formation.
Asunto(s)
Proteínas Bacterianas/genética , ADN-Topoisomerasas de Tipo II/genética , Proteínas de Unión al ADN/genética , Escherichia coli/enzimología , Alelos , Topoisomerasa de ADN IV , ADN Superhelicoidal/química , Electroforesis en Gel de Poliacrilamida , Isopropil Tiogalactósido/metabolismo , Microscopía Fluorescente , Mutación , FenotipoRESUMEN
The products of three dominant-negative alleles of parE, encoding the ATP-binding subunit of topoisomerase IV (Topo IV), were purified and their activities characterized when reconstituted with ParC to form Topo IV. The ability of the ParE E418K, ParE G419D, and ParE G442D mutant Topo IVs to bind DNA, hydrolyze ATP, and close their ATP-dependent clamp was relatively unaffected. However, their ability to relax negatively supercoiled DNA was compromised significantly. This could be attributed to severe defects in covalent complex formation between ParC and DNA. Thus, these residues, which are far from the active site Tyr of ParC, contribute to covalent catalysis. This indicates that a dramatic conformational rearrangement of the protein likely occurs subsequent to the binding of the G segment at the DNA gate and prior to its opening.
Asunto(s)
Proteínas Bacterianas/genética , ADN-Topoisomerasas de Tipo II/genética , Proteínas de Unión al ADN/genética , Escherichia coli/enzimología , Adenosina Trifosfato/metabolismo , Alelos , Proteínas Bacterianas/química , Sitios de Unión , Topoisomerasa de ADN IV , ADN-Topoisomerasas de Tipo II/química , ADN Superhelicoidal/metabolismo , Proteínas de Unión al ADN/química , Modelos Moleculares , Mutación , Norfloxacino/química , Unión Proteica/genética , Conformación ProteicaRESUMEN
Crystal structures have recently been solved of the monomeric DNA helicase PcrA bound to forked DNA, and of the hexameric helicase domain of the bacteriophage T7 gene 4 protein, a replication fork DNA helicase/primase. These structures have led to the elaboration of the first molecular models to describe DNA helicase action.
Asunto(s)
ADN Helicasas/química , ADN Helicasas/metabolismo , ADN Bacteriano/química , ADN Bacteriano/metabolismo , Proteínas Bacterianas/química , Proteínas Bacterianas/metabolismo , Simulación por Computador , ADN Primasa/química , ADN Primasa/metabolismo , Modelos Moleculares , Relación Estructura-ActividadAsunto(s)
Replicación del ADN , Proteínas de Unión al ADN/genética , Proteínas de Escherichia coli , Escherichia coli/genética , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , ADN Primasa/genética , ADN Primasa/metabolismo , Reparación del ADN/genética , ADN Bacteriano/química , ADN Bacteriano/genética , Proteínas de Unión al ADN/metabolismo , Escherichia coli/metabolismo , Modelos Genéticos , Proteína de Replicación ARESUMEN
PriA, PriB and PriC were originally discovered as proteins essential for the PhiX174 in vitro DNA replication system. Recent studies have shown that PriA mutants are poorly viable, have high basal levels of SOS expression (SOSH), are recombination deficient (Rec-), sensitive to UV irradiation (UVS) and sensitive to rich media. These data suggest that priA's role may be more complex than previously thought and may involve both DNA replication and homologous recombination. Based on the PhiX174 system, mutations in priB and priC should cause phenotypes like those seen in priA2:kan mutants. To test this, mutations in priB and priC were constructed. We found that, contrary to the PhiX174 model, del(priB)302 and priC303:kan mutants have almost wild-type phenotypes. Most unexpectedly, we then found that the priBC double mutant had very poor viability and/or a slow growth rate (even less than a priA2:kan mutant). This suggests that priB and priC have a redundant and important role in Escherichia coli. The priA2:kan suppressor, dnaC809, partially suppressed the poor viability/slow growth phenotype of the priBC double mutant. The resulting triple mutant (priBC dnaC809 ) had small colony size, recombination deficiency and levels of SOS expression similar to a priA2:kan mutant. The priBC dnaC809 mutant, however, was moderately UVR and had good viability, unlike a priA2:kan mutant. Additional mutations in the triple mutant were selected to suppress the slow growth phenotype. One suppressor restored all phenotypes tested to nearly wild-type levels. This mutation was identified as dnaC820 (K178N) [mapping just downstream of dnaC809 (E176G)]. Experiments suggest that dnaC820 makes dnaC809 suppression of priA and or priBC mutants priB and or priC independent. A model is proposed for the roles of these proteins in terms of restarting collapsed replication forks from recombinational intermediates.
Asunto(s)
Proteínas Bacterianas/genética , Replicación del ADN , ADN Bacteriano/genética , Proteínas de Unión al ADN/genética , Proteínas de Escherichia coli , Escherichia coli/genética , Proteínas Bacterianas/metabolismo , Bacteriófago mu/patogenicidad , División Celular/genética , Proteínas de Unión al ADN/metabolismo , Escherichia coli/virología , Mutación , Fenotipo , Recombinación Genética , Proteína de Replicación A , Respuesta SOS en Genética/genética , Especificidad por Sustrato , Supresión GenéticaRESUMEN
PriA is a single-stranded DNA-dependent ATPase, DNA translocase, and DNA helicase that was discovered originally because of its requirement in vitro for the conversion of bacteriophage phi X174 viral DNA to the duplex replicative form. Studies demonstrated that PriA catalyzes the assembly of a primosome, a multiprotein complex that primes DNA synthesis, on phi X174 DNA. The primosome was shown to be capable of providing both the DNA unwinding function and the Okazaki fragment priming function required for replication fork progression. However, whereas seven proteins, PriA, PriB, PriC, DnaT, DnaB, DnaC, and DnaG, were required for primosome assembly on phi X174 DNA, only DnaB, DnaC, and DnaG were required for replication from oriC, suggesting that the other proteins were not involved in chromosomal replication. Strains carrying priA null mutations, however, were constitutively induced for the SOS response, and were defective in homologous recombination, repair of UV-damaged DNA, and double-strand breaks, and both induced and constitutive stable DNA replication. The basis for this phenotype can now be explained by the ability of PriA to load replication forks at a D loop, an intermediate that forms during homologous recombination, double-strand break-repair, and stable DNA replication. Thus, a long-theorized connection between recombination and replication is demonstrated.
Asunto(s)
Replicación del ADN , Proteínas de Unión al ADN/metabolismo , Recombinación Genética , Secuencia de Bases , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Proteína de Replicación ARESUMEN
Several protein-protein interactions have been shown to be critical for proper replication fork function in Escherichia coli. These include interactions between the polymerase and the helicase, the helicase and the primase, and the primase and the polymerase. We have studied the influence of these interactions on proper initiation at oriC by using mutant primases defective in their interaction with the helicase and DNA polymerase III holoenzyme lacking the tau subunit so that it will not interact with the helicase. We show here that accurate initiation of bidirectional DNA replication from oriC is dependent on proper placement of the primers for leading strand synthesis and is thus governed primarily by the interaction between the helicase and primase.
Asunto(s)
Proteínas Bacterianas , ADN Helicasas/metabolismo , ADN Primasa/metabolismo , Replicación del ADN , Proteínas de Unión al ADN/genética , Proteínas Virales/genética , ADN Bacteriano/biosíntesis , Proteínas de Unión al ADN/biosíntesis , AdnB Helicasas , Electroforesis en Gel de Agar , Escherichia coli/genética , Complejo de Reconocimiento del Origen , Unión Proteica , Proteínas Virales/biosíntesisRESUMEN
The role of PriA, required for the assembly of the phiX174-type primosome on DNA, in cellular DNA replication has been unclear since its discovery. Recent evidence, based on the phenotypes of strains carrying priA null mutations, has led to proposals that the primosome assembly activity of PriA was required to load replication forks at intermediates such as D loops during homologous recombination. McGlynn et al. (McGlynn, P., Al-Deib, A. A., Liu, J., Marians, K. J., and Lloyd, R. G. (1997) J. Mol. Biol. 270, 212-221) demonstrated that PriA could, in fact, bind D loops. We show here that there are two modes of stable binding of PriA to DNA. One mode, in which the enzyme binds 3'-single-stranded extensions from duplex DNAs, presumably reflects the 3' --> 5' DNA helicase activity of PriA. The D loop DNA binding activity of PriA can be accounted for by the second mode, where the enzyme binds bent DNA at three strand junctions.
Asunto(s)
Adenosina Trifosfatasas/metabolismo , ADN/química , ADN Helicasas/metabolismo , Replicación del ADN , Proteínas de Unión al ADN/metabolismo , Electroforesis en Gel de Poliacrilamida , Conformación de Ácido Nucleico , OligodesoxirribonucleótidosRESUMEN
Escherichia coli strains carrying null mutations in priA are chronically induced for the SOS response and are defective in homologous recombination, repair of UV damaged DNA, double-strand break repair, and both induced and constitutive stable DNA replication. This led to the proposal that PriA directed replication fork assembly at D loops formed by the homologous recombination machinery. The demonstration that PriA specifically recognized and bound D loop DNA supported this hypothesis. Using DNA footprinting as an assay, we show here that PriA also directs the assembly of a varphiX174-type primosome on D loop DNA. The ability to load a complete primosome on D loop DNA is a step necessary for replication fork assembly.