Salmonella screening in pork slaughter flowchart

The aim of this study was to trace the sources of Salmonella contamination during the pig slaughter flowchart. Ten lots of pigs sent for slaughter were followed (four animals per lot), and two weeks before slaughter they were selected based on the presence or absence of Salmonella in their farm stalls. Stool samples were collected after stunning, and from the surface swabs of the carcass in different parts of the flowchart. Samples were also collected immediately after the animals left the dehairing machine, after opening the abdominal cavity, before the carcass entered the cooling chamber, and from jowl samples. The water samples used in the scalding tank were collected before commencing the slaughter process and after the passage of the animals. For the comparison of band patterns, the isolates were analyzed by rep-PCR. The percentage of isolation was 35.3% after stunning, 17.6% immediately after the animals left the dehairing machine, 17.6% after evisceration, 23.5% before entering the cooling chamber and 5.8% from the jowls. The serotypes obtained were: Senftenberg, Idikan, Typhimurium, Heidelberg, Minnesota, Panama and Salmonella group O:4,5. By repPCR analysis, it was found that Salmonella strains that reached the slaughterhouse in carrier pigs may not be eliminated during processing, thereby making its isolation from the carcasses possible. It was also observed that the strains introduced by animals can infect others in different stages of the slaughter flowchart, thus resulting in cross-contamination.(AU)

Salmonella screening in pork slaughter flowchart

The aim of this study was to trace the sources of Salmonella contamination during the pig slaughter flowchart. Ten lots of pigs sent for slaughter were followed (four animals per lot), and two weeks before slaughter they were selected based on the presence or absence of Salmonella in their farm stalls. Stool samples were collected after stunning, and from the surface swabs of the carcass in different parts of the flowchart. Samples were also collected immediately after the animals left the dehairing machine, after opening the abdominal cavity, before the carcass entered the cooling chamber, and from jowl samples. The water samples used in the scalding tank were collected before commencing the slaughter process and after the passage of the animals. For the comparison of band patterns, the isolates were analyzed by rep-PCR. The percentage of isolation was 35.3% after stunning, 17.6% immediately after the animals left the dehairing machine, 17.6% after evisceration, 23.5% before entering the cooling chamber and 5.8% from the jowls. The serotypes obtained were: Senftenberg, Idikan, Typhimurium, Heidelberg, Minnesota, Panama and Salmonella group O:4,5. By repPCR analysis, it was found that Salmonella strains that reached the slaughterhouse in carrier pigs may not be eliminated during processing, thereby making its isolation from the carcasses possible. It was also observed that the strains introduced by animals can infect others in different stages of the slaughter flowchart, thus resulting in cross-contamination.

Qualidade microbiológica de queijo mussarela em peça e fatiado / Microbiological quality of sliced and block mozzarella cheese

O objetivo deste trabalho foi verificar o padrão microbiológico de queijo mussarela comercializado em estabelecimentos varejistas de Pelotas, Rio Grande do Sul. Foram analisadas 40 amostras de queijo mussarela, compreendendo 20 de queijo em peça e 20 de queijo fatiado, as quais foram submetidas a contagem de coliformes termotolerantes e de estafilococos coagulase positiva, e a pesquisa de Salmonella spp. e de Listeria monocytogenes. Observou-se que 12,5% das amostras de queijo fatiado e 5% de queijo em peça estavam em desacordo com os padrões estabelecidos pela legislação brasileira. Estes resultados indicam a necessidade de maior monitoramento desses produtos e maior cuidado higiênico-sanitário durante o processamento por parte das indústrias.(AU)

The aim of this study was to verify the microbiological quality of mozzarella cheese sold in retail markets of Pelotas, Rio Grande do Sul, Brazil. Forty samples of mozzarella cheese were analyzed, comprising 20 samples of block cheese and 20 of sliced cheese. The cheese samples were analyzed for thermotolerant coliform counts and coagulase positive staphylococci counts, and presence of Salmonella spp and Listeria monocytogenes. The percentage of 12,5% and 5% of the sliced and block cheese samples analyzed, respectively, exceeded the microbiological standards accepted by Brazilian legislation. These results indicate the need for a better product monitoring and more concern with hygiene and sanitary practices during industrial process.(AU)

Qualidade microbiológica de queijo mussarela em peça e fatiado / Microbiological quality of sliced and block mozzarella cheese

O objetivo deste trabalho foi verificar o padrão microbiológico de queijo mussarela comercializado em estabelecimentos varejistas de Pelotas, Rio Grande do Sul. Foram analisadas 40 amostras de queijo mussarela, compreendendo 20 de queijo em peça e 20 de queijo fatiado, as quais foram submetidas a contagem de coliformes termotolerantes e de estafilococos coagulase positiva, e a pesquisa de Salmonella spp. e de Listeria monocytogenes. Observou-se que 12,5% das amostras de queijo fatiado e 5% de queijo em peça estavam em desacordo com os padrões estabelecidos pela legislação brasileira. Estes resultados indicam a necessidade de maior monitoramento desses produtos e maior cuidado higiênico-sanitário durante o processamento por parte das indústrias.

The aim of this study was to verify the microbiological quality of mozzarella cheese sold in retail markets of Pelotas, Rio Grande do Sul, Brazil. Forty samples of mozzarella cheese were analyzed, comprising 20 samples of block cheese and 20 of sliced cheese. The cheese samples were analyzed for thermotolerant coliform counts and coagulase positive staphylococci counts, and presence of Salmonella spp and Listeria monocytogenes. The percentage of 12,5% and 5% of the sliced and block cheese samples analyzed, respectively, exceeded the microbiological standards accepted by Brazilian legislation. These results indicate the need for a better product monitoring and more concern with hygiene and sanitary practices during industrial process.