Detection of micronuclei in gill cells and haemocytes of mussels exposed to benzo[a]pyrene.

Mediterranean mussels were exposed to benzo[a]pyrene for 2 days at doses which had previously caused the formation of specific adducts in gill DNA. Micronuclei and other nuclear abnormalities were detected in gill cells and haemocytes in order to ascertain the induction of cytogenetic damage in two different target cells in parallel. A number of procedural details were examined initially to improve the quality of slides obtained from mussel cells. Adequate cytological preparations were obtained when gill cells and haemocytes were suspended, respectively, in Alsever and sea water with EDTA, cytospun and fixed with absolute methanol. In the exposed mussels, micronuclei significantly increased in both the large gill cells (the main cell type) and the agranular haemocytes. Granular haemocytes, cells present in variable proportions between individual mussels, did not show cytogenetic damage except at the highest B[a]P doses. In the same slides, steady levels of binucleated cells were detected, whereas the incidence of other nuclear abnormalities was significantly higher in the exposed compared with control mussels. Precise knowledge of the replication kinetics of gill cells and haemocytes is still lacking.

A multidimensional approach to the study of the structure of coping with stress.

This article described a study in which the responses of 199 respondents to measures of coping with stress were submitted to multidimensional scaling. The three-dimensional solution suggests that respondents' perceptions of coping with stress are best explained along the following dimensions: (a) active coping versus denial/escape, (b) turning to interpersonal support versus seeking religious comfort, and (c) instrumental/cognitive disengagement versus affective/spiritual engagement.

Progeria: medical aspects, psychosocial perspectives, and intervention guidelines.

This article discusses progeria, a rare genetic childhood disorder that invariably results in the individual's death during early adolescence. The article begins by describing the major medical aspects of progeria. This is followed by a discussion of the psychosocial implications of the disorder with particular emphasis upon grief-triggered reactions. The article concludes with an overview of psychosocial intervention guidelines for caregivers who work with families of dying children and adolescents.

Splenic rupture due to extraperitoneal gunshot wound: use of peritoneal lavage in the low-tech environment.

The upsurge in armed conflicts and civil unrest around the world will result in the need to treat gunshot wounds in austere conditions. This report details a splenic injury that resulted from a gunshot of the torso that did not penetrate the peritoneum. The author made the diagnosis using peritoneal lavage, which can be adapted to field conditions.