RESUMEN
In this work, we report the synthesis of a peptide analogue of the KTTKS, termed Ac- Wahx-KTTKS and evaluate its cytotoxicity and role in biosynthesis of collagen for future application in skin aging. The peptide was obtained with purity higher than 97.5%. In the cytotoxicity assay, we observed non-toxic effects for Ac-WAhx-KTTKS at concentrations below 600 µM for HaCaT and 500 µM for HepG2 cells, respectively. After 24 and 48 h it was possible to observe significant changes in collagen synthesis in the groups treated with various concentrations of the peptide. In conclusion, the Ac-Wahx- KTTKS peptide increased collagen synthesis in fibroblasts by 80% and it is a promising candidate for improving skin aging.
Asunto(s)
Colágeno/metabolismo , Fibroblastos/efectos de los fármacos , Oligopéptidos/síntesis química , Oligopéptidos/farmacología , Envejecimiento de la Piel/efectos de los fármacos , Piel/efectos de los fármacos , Acetilación , Línea Celular Tumoral , Colágeno/biosíntesis , Células Hep G2 , Humanos , Oligopéptidos/efectos adversos , Piel/metabolismo , Envejecimiento de la Piel/patologíaRESUMEN
Cathepsin V is a papain-like cysteine protease. It is involved in the control of human T cells (responsible for cell immunity), and presents the largest elastolytic activity among the proteolytic enzymes. Therefore, cathepsin V is a potential molecular target for the treatment of atherosclerosis. In the present work, natural flavonoids were screened against cathepsin V, and two flavones were identified as potent inhibitors of cathepsin V. On the basis of this result, a combinatorial library of chalcones and flavones was prepared, in solution phase employing a scavenger reagent, and fully evaluated.