RESUMEN
Hypoxic ischaemic encephalopathy (HIE) is a significant cause of death and disability. Therapeutic hypothermia (TH) is the only available standard of treatment, but 45-55% of cases still result in death or neurodevelopmental disability following TH. This work has focussed on developing a new brain tissue physiology and biochemistry systems biology model that includes temperature effects, as well as a Bayesian framework for analysis of model parameter estimation. Through this, we can simulate the effects of temperature on brain tissue oxygen delivery and metabolism, as well as analyse clinical and experimental data to identify mechanisms to explain differing behaviour and outcome. Presented here is an application of the model to data from two piglets treated with TH following hypoxic-ischaemic injury showing different responses and outcome following treatment. We identify the main mechanism for this difference as the Q10 temperature coefficient for metabolic reactions, with the severely injured piglet having a median posterior value of 0.133 as opposed to the mild injury value of 5.48. This work demonstrates the use of systems biology models to investigate underlying mechanisms behind the varying response to hypothermic treatment.
Asunto(s)
Hipotermia Inducida , Hipoxia-Isquemia Encefálica , Animales , Teorema de Bayes , Hipoxia-Isquemia Encefálica/terapia , Oxígeno , Porcinos , Biología de SistemasRESUMEN
An anatomical model of the dissection of the human neck, supraclavicular and submandibular regions, was studied in order to determine its anatomical accuracy. The sample was made of brightly coloured wax and was of female appearance. Formally, it belonged to the collection (n(o) 23) of a medical doctor: Spitzner (1813-1894). It was donated late in the 20th century to the Delmas, Orfila and Rouvière museums, 45, rue des Saints-Pères, Paris 6(e), université Paris-Descartes (catalog n(o): 1222, SRA-1995). It measured 36cm in width and 27cm in height. With the camera, it was pictured on its main views and every 5-degree on 270° along its vertical axis in order to obtain a tri-dimensional, photographic reconstruction. The arterial and venous vessels were enhanced and the thyroid and submandibular glands as well. The musculo-osseous frames limited five main regions of dissection. Neither nerves nor lymphatics were represented. The author of this wax sample remains unknown (no signature). The aspect does not look like the samples of the Tramond or Auzoux's factories. The Spitzner collection was primarily an itinerant exhibition; however, it is of a highly anatomical quality. It is well known by the anatomical art world, especially abroad: its aesthetical features and facial characters make it very recognizable.
