A SAS code to estimate phenotypic-genotypic covariance and correlation matrices based on expected value of statistical designs to use in plant breeding.

Phenotypic-genotypic covariance and correlation have been useful in crop and animal breeding programs. In the study of diversity of natural populations and different cultivars of plants that are examined based on statistical design, estimation of genotypic-phenotypic covariance through expected value of statistical designs mean square is hard and time-consuming when the number of studied traits is high. Moreover, the lack of a program in this field and manual calculations make the estimation more complicated. Therefore, in this study, one program was developed in SAS that can be used to calculate the genotypic-phenotypic covariance matrix through the first part of the program based on the expected value of applied statistical designs mean square. Then, based on the covariance matrix computed from the previous design model, their correlation matrix was calculated using the second part of the program based on the interactive matrix language (IML) of SAS. The phenotypic-genotypic covariance matrices of the 12 studied traits of rice are calculated based on this code. This program could compute phenotypic-genotypic covariance and correlation matrices based on the expected value of any statistical designs.

Multi-trait and multi-environment QTL analyses for resistance to wheat diseases.

BACKGROUND: Stripe rust, leaf rust, tan spot, and Karnal bunt are economically significant diseases impacting wheat production. The objectives of this study were to identify quantitative trait loci for resistance to these diseases in a recombinant inbred line (RIL) from a cross HD29/WH542, and to evaluate the evidence for the presence loci on chromosome region conferring multiple disease resistance. METHODOLOGY/PRINCIPAL FINDINGS: The RIL population was evaluated for four diseases and genotyped with DNA markers. Multi-trait (MT) analysis revealed thirteen QTLs on nine chromosomes, significantly associated with resistance. Phenotypic variation explained by all significant QTLs for KB, TS, Yr, Lr diseases were 57%, 55%, 38% and 22%, respectively. Marginal trait analysis identified the most significant QTLs for resistance to KB on chromosomes 1BS, 2DS, 3BS, 4BL, 5BL, and 5DL. Chromosomes 3AS and 4BL showed significant association with TS resistance. Significant QTLs for Yr resistance were identified on chromosomes 2AS, 4BL and 5BL, while Lr was significant on 6DS. MT analysis revealed that all the QTLs except 3BL significantly reduce KB and was contributed from parent HD29 while all resistant QTLs for TS except on chromosomes 2DS.1, 2DS.2 and 3BL came from WH542. Five resistant QTLs for Yr and six for Lr were contributed from parents WH542 and HD29 respectively. Chromosome region on 4BL showed significant association to KB, TS, and Yr in the population. The multi environment analysis for KB identified three putative QTLs of which two new QTLs, mapped on chromosomes 3BS and 5DL explained 10 and 20% of the phenotypic variation, respectively. CONCLUSIONS/SIGNIFICANCE: This study revealed that MT analysis is an effective tool for detection of multi-trait QTLs for disease resistance. This approach is a more effective and practical than individual QTL mapping analyses. MT analysis identified RILs that combine resistance to multiple diseases from parents WH542 and/or HD29.

Manejo anestésico de las emergencias de oftalmología / Anaesthesic treatment of the ophtalmologic emergencies

Se realizó un estudio prospectivo sobre el manejo anestésico de los pacientes intervenidos bajo anestesia general por traumas oculares agudos durante un período de tres meses en el Hospital Dr. Luis E. Aybar, con la intención de evaluar la calidad de la anestesia, sobretodo por la forma del paciente recobrar su estado de conciencia. Por su condición de emergencia los pacientes no fueron premedicados, se les administró anestesia general endotraqueal con Tiopental o Ketamina o anestesia general endovenosa con Diazepam o Ketamina. El criterio que prevaleció para determinar la técnica anestésica fue de la edad del paciente, duración del procedimiento, carácter intra o extraocular de la intervención y status hemático. La técnica anestésica general endotraqueal se empleó en 60 pacientes para un 83.3//y la general endovenosa en 12 para un 16.///. En 56 de los pacientes (77.7//) el diagnóstico prequirurgico fue herida de cornea. Todos los pacientes en este estudio fueron menores de 15 años de edad. Ningún paciente tuvo una mala recuperación de la anestesia, 16.7//tuvo una recuperación regular y 83.3//una recuperación buena