RESUMEN
We describe a mathematical model of signal from single-channel direct hybridization microarray platforms. The model establishes a linear relationship between microarray signals and their standard deviations from a minimum set of assumptions. We use the model to precisely define important microarray quality characteristics: resolved fold change and dynamic range. The definitions lead to closed form expressions relating these characteristics to physical parameters of the microarray experiment in the case when both specific and nonspecific binding of target to probe are governed by the Langmuir hybridization isotherm. The predictions of the model are in close agreement to data obtained from spike-in experiments. Given the generality of the model, the introduced definitions of dynamic range and resolved concentration fold-change can be used to conduct cross-platform comparisons and to guide improvement of the microarray platform.
Asunto(s)
Biología Computacional , Modelos Genéticos , Análisis de Secuencia por Matrices de Oligonucleótidos/estadística & datos numéricos , Interpretación Estadística de DatosRESUMEN
BACKGROUND: Because of limited genetic tools for use in Helicobacter pylori, tests routinely applied in other bacteria for demonstrating a gene's role in viability and other phenotypes have not been applied to this organism. In a mutational study of putative response regulator genes, we aimed to develop such tools for H. pylori. MATERIALS AND METHODS: We attempted to mutate five response regulator genes by allelic exchange insertional mutagenesis. For genes that yielded no viable mutants, a second copy of the gene was inserted into the chromosome via a suicide vector, and it was seen if providing the second copy would permit the gene's disruption. For genes that yielded mutants with selectable phenotypes, a strategy was developed for reversion whereby an intact copy of the gene is introduced to the organism by transformation with PCR products. Following this procedure, revertants were selected by phenotypic tests then tested for genetic reversion. RESULTS: After failure to attain transformants upon attempted mutation of genes HP0166 and HP1365, we inserted a second copy of each gene within the H. pylori chromosome. In each case the second copy relieved the block of transformation. Mutation of genes HP0703 and HP1021 gave non-motile and small-colony phenotypes, respectively. Following transformation with PCR products containing intact copies of the genes, both phenotype and genotype had reverted following phenotypic selections. CONCLUSIONS: The methods used in this study provide new approaches for confirming suspected genotype/phenotype associations and should be widely applicable in the study of H. pylori.
Asunto(s)
Genes Bacterianos , Genes Letales , Genes Reguladores , Helicobacter pylori/genética , Animales , Células Cultivadas , Cromosomas Bacterianos/genética , Ensayo de Inmunoadsorción Enzimática , Mucosa Gástrica/microbiología , Vectores Genéticos , Genotipo , Helicobacter pylori/crecimiento & desarrollo , Caballos , Humanos , Interleucina-8/metabolismo , Mutagénesis Insercional , Mutación , Fenotipo , Reacción en Cadena de la Polimerasa , Transformación BacterianaRESUMEN
Helicobacter pylori colonizes the stomach of half of the world's population, causing a wide spectrum of disease ranging from asymptomatic gastritis to ulcers to gastric cancer. Although the basis for these diverse clinical outcomes is not understood, more severe disease is associated with strains harboring a pathogenicity island. To characterize the genetic diversity of more and less virulent strains, we examined the genomic content of 15 H. pylori clinical isolates by using a whole genome H. pylori DNA microarray. We found that a full 22% of H. pylori genes are dispensable in one or more strains, thus defining a minimal functional core of 1281 H. pylori genes. While the core genes encode most metabolic and cellular processes, the strain-specific genes include genes unique to H. pylori, restriction modification genes, transposases, and genes encoding cell surface proteins, which may aid the bacteria under specific circumstances during their long-term infection of genetically diverse hosts. We observed distinct patterns of the strain-specific gene distribution along the chromosome, which may result from different mechanisms of gene acquisition and loss. Among the strain-specific genes, we have found a class of candidate virulence genes identified by their coinheritance with the pathogenicity island.
Asunto(s)
Variación Genética , Genoma Bacteriano , Helicobacter pylori/genética , ADN Bacteriano/análisis , Genes Bacterianos , Helicobacter pylori/patogenicidad , Humanos , Análisis de Secuencia por Matrices de Oligonucleótidos/métodosRESUMEN
Regulation of virulence gene expression in enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) is incompletely understood. In EPEC, the plasmid-encoded regulator Per is required for maximal expression of proteins encoded on the locus of enterocyte effacement (LEE), and a LEE-encoded regulator (Ler) is part of the Per-mediated regulatory cascade upregulating the LEE2, LEE3, and LEE4 promoters. We now report that Ler is essential for the expression of multiple LEE-located genes in both EPEC and EHEC, including those encoding the type III secretion pathway, the secreted Esp proteins, Tir, and intimin. Ler is therefore central to the process of attaching and effacing (AE) lesion formation. Ler also regulates the expression of LEE-located genes not required for AE-lesion formation, including rorf2, orf10, rorf10, orf19, and espF, indicating that Ler regulates additional virulence properties. In addition, Ler regulates the expression of proteins encoded outside the LEE that are not essential for AE lesion formation, including TagA in EHEC and EspC in EPEC. delta ler mutants of both EPEC and EHEC show altered adherence to epithelial cells and express novel fimbriae. Ler is therefore a global regulator of virulence gene expression in EPEC and EHEC.
Asunto(s)
Mapeo Cromosómico , Escherichia coli/genética , Escherichia coli/patogenicidad , Genes Bacterianos , Genes Reguladores , Proteínas/genética , Secuencia de Aminoácidos , Adhesión Bacteriana , Secuencia de Bases , Fimbrias Bacterianas/fisiología , Regulación Bacteriana de la Expresión Génica , Datos de Secuencia Molecular , VirulenciaRESUMEN
The severity of Helicobacter pylori-related disease is correlated with a pathogenicity island (the Cag region of about 26 genes) whose presence is associated with the up-regulation of an IL-8 cytokine inflammatory response in gastric epithelial cells. Statistical analysis of the Cag gene sequences calculated from the complete genome of strain 26695 revealed several unusual features. The Cag7 sequence (1,927 aa) has two repeat regions. Repeat region I runs 317 aa in a form of AAA proximal to the protein N terminal; repeat region II extends 907 aa in the middle of the protein sequence consisting of 74 contiguous segments composed from selections among six consensus sequences and includes 58 regularly distributed cysteine residues with consecutive cysteines mostly 12, 18, or 24 aa apart. This "regular" cysteine arrangement may provide a scaffolding of linker elements stabilized by disulfide bridges. When Cag7 homologues from different strains are compared, differences were found almost exclusively in the repeat regions, resulting from deletion and/or insertion of repeating units. These observations suggest that the anomalous repetitive structure of the sequence plays an important role in the conformation of Cag7 gene product and potentially in the function of the pathogenicity island. Other facets of the Cag7 sequence show significant charge clusters, high multiplet count, and extremes of amino acid usage.
Asunto(s)
Proteínas Bacterianas/genética , Genes Bacterianos , Genoma Bacteriano , Helicobacter pylori/genética , Secuencia de Aminoácidos , Secuencia de Bases , Mucosa Gástrica/microbiología , Infecciones por Helicobacter/microbiología , Helicobacter pylori/patogenicidad , Humanos , Datos de Secuencia Molecular , Familia de Multigenes , Secuencias Repetitivas de Ácidos Nucleicos , Eliminación de Secuencia , Virulencia/genéticaAsunto(s)
Proteínas Bacterianas/genética , Escherichia coli/genética , Escherichia coli/patogenicidad , Genes Bacterianos , Secuencia de Aminoácidos , Adhesión Bacteriana/genética , Proteínas Bacterianas/metabolismo , Datos de Secuencia Molecular , Sistemas de Lectura Abierta/genética , Relación Estructura-Actividad , Virulencia/genéticaRESUMEN
The locus of enterocyte effacement pathogenicity island confers the attaching and effacing histopathology on epithelial cells infected with enteropathogenic and enterohemorrhagic Escherichia coli. We investigated the site of insertion of the locus of enterocyte effacement in E. coli strains in relation to their evolution based on conservation of housekeeping proteins in these strains. The results indicate that the insertion site of the locus of enterocyte effacement varies according to the evolutionary lineage, suggesting that it has inserted at multiple times and sites during the evolution of these pathogens.
Asunto(s)
Escherichia coli/genética , Escherichia coli/patogenicidad , Intestinos/microbiología , Adhesión Bacteriana , Secuencia de Bases , Sitios de Unión , Cartilla de ADN/genética , ADN Bacteriano/genética , Células Epiteliales/microbiología , Células Epiteliales/patología , Escherichia coli/clasificación , Infecciones por Escherichia coli/microbiología , Infecciones por Escherichia coli/patología , Evolución Molecular , Hemorragia Gastrointestinal/microbiología , Hemorragia Gastrointestinal/patología , Humanos , Enfermedades Intestinales/microbiología , Enfermedades Intestinales/patología , Intestinos/patología , Filogenia , Reacción en Cadena de la Polimerasa , Virulencia/genéticaRESUMEN
Attaching and effacing (AE) bacteria are a diverse group of gastrointestinal pathogens, comprising members of four genera, that cause the intestinal epithelial microvilli to be replaced with raised clusters of filamentous actin that conform to the surface of attached bacteria. We have cloned a 35.4 kb 'pathogenicity island' from the prototype AE bacterium, enteropathogenic Escherichia coli, containing all previously described AE genes. Transfer of this pathogenicity island to avirulent E. coli converts the recipients into strains that secrete virulence proteins, induce host signal-transduction pathways, and cause AE lesions on cultured epithelial cells. These results demonstrate that this pathogenicity island contains all pathogen-specific genes necessary for inducing AE lesions, and that the defining feature of this class of pathogens can be acquired by an avirulent bacterium in a single genetic step.
Asunto(s)
Escherichia coli/patogenicidad , Transformación Bacteriana , Adhesión Bacteriana , Clonación Molecular , Microscopía Electrónica , Microscopía Fluorescente , Fenotipo , VirulenciaRESUMEN
EPEC Escherichia coli are an important cause of epidemic diarrhea in infants. The disease is characterized by attaching and effacing (A/E) lesions where the bacteria attach intimately to the enterocyte surface resulting in localized destruction of microvilli. A 35-kb chromosomal locus termed LEE (locus of enterocyte effacement) in an EPEC strain (E2348/69) has recently been found and is thought to contain all the necessary genes for A/E lesions. RDEC-1 is a strain of E. coli that causes diarrhea in rabbits by a similar mechanism and serves as a model for human EPEC disease. We report 1) the cloning of the RDEC-1 LEE, 2) show that the RDEC-1 LEE is similar in size to the LEE in E2348/69, 3) the RDEC-1 LEE possesses all four regions of the E2348/69 LEE, 4) there are restriction site polymorphisms between the RDEC-1 LEE and that of E2348/69, and 5) the RDEC-1 LEE clone is functionally similar to E2348/69 in its fluorescent actin staining test and suggests that the LEE may be sufficient for the production of A/E lesions by these strains.
Asunto(s)
Adhesión Bacteriana , Diarrea Infantil/microbiología , Infecciones por Escherichia coli/microbiología , Escherichia coli/genética , Línea Celular , Clonación Molecular , ADN Bacteriano/genética , Escherichia coli/patogenicidad , Genes Bacterianos , Humanos , Lactante , Recién Nacido , Polimorfismo de Longitud del Fragmento de RestricciónRESUMEN
Enteropathogenic E. coli (EPEC) are a major cause of diarrhea in infants throughout the world. Although this pathogen was described 50 years ago, it is only recently that the pathogenic mechanisms employed by this organism have been elucidated. The characteristic histopathology induced by this organism, called "attaching and effacing", consists of intimate adherence of the bacterium to the epithelial cell with marked cytoskeletal changes including effacement of microvilli. A 35 kb region of chromosomal DNA, called the LEE for locus of enterocyte effacement has recently been described which contains all known genes necessary for production of this characteristic histopathology. Within this region is the eae gene encoding intimin, a 94 kDa OMP involved in intimate adherence. Also within this region are genes encoding proteins secreted extracellularly by EPEC (esp) and a type III secretion apparatus (sep) which shares homology with similar systems in Yersinia, Shigella, and Salmonella. Additional genes on a 60 MDa plasmid encode a type IV pilus (BFP) and a positive transcriptional activator (per) of multiple chromosomal and plasmid virulence genes.
Asunto(s)
Diarrea/microbiología , Infecciones por Escherichia coli/fisiopatología , Escherichia coli/genética , Escherichia coli/patogenicidad , Animales , Adhesión Bacteriana , Células Cultivadas , ADN Bacteriano/genética , Regulación Bacteriana de la Expresión Génica , Genes Bacterianos , Enfermedades Intestinales/microbiología , PlásmidosRESUMEN
An unusual mutant of enteropathogenic E. coli (EPEC), deficient in its ability to invade host cells, was evaluated. The gene interrupted by the transposon in this mutant was located within a region of the EPEC chromosome devoted to secretion of proteins required for signal transduction. The mutant did not secrete detectable levels of the EspB protein, previously shown to be required for attaching and effacing, and did not induce detectable tyrosine phosphorylation of a 90 kDa host cell protein, previously associated with attaching and effacing and invasion. No quantitative or qualitative defect in the ability of the mutant to induce attaching and effacing effects was observed. Moreover, attaching and effacing by wild-type EPEC was unaffected by high doses of the tyrosine kinase inhibitor genistein. These results indicate that attaching and effacing activity can occur in the absence of detectable EspB secretion and tyrosine kinase mediated signal transduction.
Asunto(s)
Adhesión Bacteriana/genética , Proteínas Bacterianas/genética , Proteínas de Escherichia coli , Escherichia coli/genética , Genes Bacterianos , Mutación , Actinas/metabolismo , Secuencia de Aminoácidos , Proteínas de la Membrana Bacteriana Externa/metabolismo , Secuencia de Bases , Células Cultivadas , Clonación Molecular , Inhibidores Enzimáticos/farmacología , Escherichia coli/patogenicidad , Prueba de Complementación Genética , Intestinos/microbiología , Datos de Secuencia Molecular , Mutagénesis Insercional , Proteínas Tirosina Quinasas/antagonistas & inhibidores , Proteínas Tirosina Quinasas/metabolismo , Análisis de Secuencia de ADN , Transducción de SeñalRESUMEN
Enteropathogenic Escherichia coli (EPEC) causes a characteristic histopathology in intestinal epithelial cells called the attaching and effacing lesion. Although the histopathological lesion is well described the bacterial factors responsible for it are poorly characterized. We have identified four EPEC chromosomal genes whose predicted protein sequences are similar to components of a recently described secretory pathway (type III) responsible for exporting proteins lacking a typical signal sequence. We have designated the genes sepA, sepB, sepC, and sepD (sep, for secretion of E. coli proteins). The predicted Sep polypeptides are similar to the Lcr (low calcium response) and Ysc (yersinia secretion) proteins of Yersinia species and the Mxi (membrane expression of invasion plasmid antigens) and Spa (surface presentation of antigens) regions of Shigella flexneri. Culture supernatants of EPEC strain E2348/69 contain several polypeptides ranging in size from 110 kDa to 19 kDa. Proteins of comparable size were recognized by human convalescent serum from a volunteer experimentally infected with strain E2348/69. A sepB mutant of EPEC secreted only the 110-kDa polypeptide and was defective in the formation of attaching and effacing lesions and protein-tyrosine phosphorylation in tissue culture cells. These phenotypes were restored upon complementation with a plasmid carrying an intact sepB gene. These data suggest that the EPEC Sep proteins are components of a type III secretory apparatus necessary for the export of virulence determinants.
Asunto(s)
Proteínas Bacterianas/biosíntesis , Proteínas Bacterianas/fisiología , Proteínas de Escherichia coli , Escherichia coli/fisiología , Escherichia coli/patogenicidad , Genes Bacterianos , Secuencia de Aminoácidos , Animales , Anticuerpos , Proteínas Bacterianas/genética , Secuencia de Bases , Clonación Molecular , ADN Bacteriano/química , ADN Bacteriano/metabolismo , Epitelio/microbiología , Humanos , Immunoblotting , Intestinos/microbiología , Datos de Secuencia Molecular , Mutagénesis Insercional , Conejos/inmunología , Proteínas Recombinantes/biosíntesis , Mapeo Restrictivo , Shigella flexneri/patogenicidad , Shigella flexneri/fisiología , Yersinia/patogenicidad , Yersinia/fisiologíaRESUMEN
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli O157:H7 are intestinal pathogens that profoundly damage the microvilli and subapical cytoskeleton of epithelial cells. Here we report finding in EPEC a 35-kbp locus containing several regions implicated in formation of these lesions. DNA probes throughout this locus hybridize to E. coli O157:H7 and other pathogens of three genera that cause similar lesions but do not hybridize to avirulent members of the same species. The EPEC locus and a different virulence locus of uropathogenic E. coli insert into the E. coli chromosome at the identical site and share highly similar sequences near the point of insertion.
Asunto(s)
ADN Bacteriano/genética , Enteritis/microbiología , Escherichia coli/patogenicidad , Genes Bacterianos , Secuencia de Bases , Mapeo Cromosómico , Elementos Transponibles de ADN , Enteritis/patología , Epitelio/patología , Escherichia coli/genética , Datos de Secuencia Molecular , Mutagénesis Insercional , Mapeo RestrictivoRESUMEN
Loss of heterozygosity occurring on various chromosomes has been described in the majority of human tumors. The targets of frequent or consistent subchromosomal deletions are believed to be tumor suppressor genes. We examined 72 esophageal tumors (46 squamous cell carcinomas and 26 adenocarcinomas) for loss of heterozygosity at the p53, Rb, APC, MCC, and DCC loci. Inclusion of these tumor suppressor genes in the allelic deletions was directly ascertained by performing polymerase chain reaction at polymorphic sites within the genes. Loss of heterozygosity occurred in 55% of informative cases at p53, in 48% of informative cases at Rb, in 66% at APC, in 63% at MCC, and in 24% at DCC. Ninety-three % of tumors informative at all loci (fully informative) lost heterozygosity of at least one locus. A high percentage of fully informative tumors (71%) also lost heterozygosity at more than one locus. There were no significant differences among histological types in the prevalence of loss of heterozygosity at any locus. There were correlations of losses involving MCC versus DCC, Rb, and p53. These data suggest that (a) allelic deletions including these tumor suppressor genes are important in the formation and/or progression of most esophageal cancers; (b) allelic deletions involving MCC may not occur independently of deletions involving other tumor suppressor genes; and (c) the accumulation of multiple allelic deletions involving specific tumor suppressor genes may be important in most esophageal tumorigenesis or tumor evolution.
Asunto(s)
Adenocarcinoma/genética , Carcinoma de Células Escamosas/genética , Neoplasias Esofágicas/genética , Eliminación de Gen , Genes Supresores de Tumor , Genes p53 , Heterocigoto , Secuencia de Bases , Southern Blotting , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la PolimerasaRESUMEN
We describe a method for rapid radioactive labeling of PCR product. The method, employing the Klenow fragment of DNA polymerase I, consumes little product, requires no product purification and takes under 30 minutes.
Asunto(s)
ADN Polimerasa I , Reacción en Cadena de la Polimerasa/métodos , Radioisótopos de Fósforo , Secuencias Repetitivas de Ácidos NucleicosRESUMEN
Loss of heterozygosity affecting chromosome 17p has been detected at high frequencies in a variety of human tumors, including cancers of the colon, breast, lung, and brain. One presumed target of these losses is p53, a tumor suppressor gene located on 17p. To our knowledge, loss of heterozygosity has not yet been reported at any locus, including p53, in human esophageal cancer. Moreover, current methods of detecting loss of heterozygosity depend on the availability of large amounts of high molecular weight DNA, making the study of small biopsy specimens or paraffin-embedded tissues problematic. We examined 52 primary human esophageal neoplasms for loss of heterozygosity affecting the p53 gene by using the polymerase chain reaction. Loss of one allele was detected in 52% of informative cases and was more common in squamous carcinomas than in adenocarcinomas. Southern blot analysis was used to confirm polymerase chain reaction-derived data. The identification of allelic loss in approximately half of the tumors analyzed supports the hypothesis that inactivation of p53 is involved in the pathogenesis of esophageal cancer.