RESUMEN
Three bovine rotavirus strains belonging to two distinct serotype groups, serotype 6 (NCDV and B641) and B223, distinct from the other six mammalian rotavirus serotypes but not yet assigned to a serotype group, were compared with each other and with canine rotavirus (K9, serotype 3) by studying the properties of their cognate polypeptide species VP4, VP6, and VP7. The three viruses showed distinct differences in the polyacrylamide gel electrophoretic migration rates of protein species VP4 and VP7, with minor differences in VP6. Differences were also observed among the migration patterns of genome segments 4, 6, and the 7-8-9 triplet, which encode VP4, VP6, and VP7, respectively. Monoclonal antibodies (MAbs) to B223, which were directed against VP4 or VP7, showed homologous specificity for neutralization and immunofluorescence (IF), although one MAb reactive with VP4 also reacted by IF and by immunoprecipitation (IP) with all four viruses and weakly neutralized B641 and K9. This MAb may react with the epitope responsible for the B223-induced one-way neutralizing and protection response of calves against B641 observed in earlier studies. MAbs reactive with VP6 by IP showed enzyme-linked immunosorbent assay and IF reactivity with all three bovine viruses and the canine virus. The two serotype 6 viruses could be distinguished by the two B641 MAbs, B641-N2b reacting by neutralization and IF with both viruses and B641-N1 reacting with B641 and the serotype 3 canine rotavirus but not with NCDV. One nonneutralizing B641 MAb reacted by IP and IF with VP7 of all four rotaviruses examined, and one B223 MAb neutralized B223 and, to low titer, B641 and K9 although reacting by IP and IF with all four viruses. Three MAb-resistant mutants were selected by passage of B223 in the presence of one of three selected B223 MAbs at concentrations which only neutralized approximately 90% of the infectious virions. The resulting mutants were 100% resistant to neutralization with their respective MAb but remained neutralizable by the same selection of MAbs as the parent B223 virus.
Asunto(s)
Antígenos Virales/análisis , Cápside/inmunología , Rotavirus/inmunología , Animales , Anticuerpos Monoclonales/inmunología , Cápside/química , Bovinos , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción Enzimática , Epítopos/análisis , Técnica del Anticuerpo Fluorescente , Pruebas de Neutralización , Péptidos/análisis , Pruebas de Precipitina , Valor Predictivo de las Pruebas , ARN Viral/análisis , Rotavirus/genéticaRESUMEN
Homologous and heterologous active immunity was studied in mice with mammalian group A rotaviruses. One day old mice were vaccinated with one of the following rotaviruses: bovine B641 (serotype 6), bovine B223 (untyped), simian SA11 (serotype 3) and murine EDIM (untyped). At 10 days of age they were challenged with EDIM virulent virus or SA11 virus. All the vaccines induced a serological antibody response in the mice but only the homologous immune response was protective.
Asunto(s)
Animales Recién Nacidos/inmunología , Rotavirus/inmunología , Animales , Células Cultivadas , Diarrea/microbiología , Ratones , Rotavirus/clasificación , Infecciones por Rotavirus/inmunología , Infecciones por Rotavirus/prevención & control , Serotipificación , Especificidad de la Especie , VacunaciónRESUMEN
In a study of the role of predisposing factors and gonadal hormones in the buller syndrome, it was found that weather, amount of space available to each steer, and entry weights of the steers were not associated with increased occurrence of bulling; however, the type and timing of administration of the hormonal implant, the number of steers in a pen, and the manner in which the steers were grouped after arrival at the feedlot were found to influence the incidence of the syndrome. Serum concentrations of estradiol and testosterone in the buller steers were found to be lower during bulling than after a short period of isolation and apparent recovery from the syndrome. It was concluded that the occurrence of bulling is related to the use of hormonal implants and certain feedlot management procedures but that it is apparently not related to increased serum estrogen or testosterone concentrations.