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1.
Braz J Med Biol Res ; 46(9): 780-8, 2013 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24068194

RESUMEN

4-Nerolidylcatechol (4-NC) is found in Pothomorphe umbellata root extracts and is reported to have a topical protective effect against UVB radiation-induced skin damage, toxicity in melanoma cell lines, and antimalarial activity. We report a comparative study of the antioxidant activity of 4-NC and α-tocopherol against lipid peroxidation initiated by two free radical-generating systems: 2,2'-azobis(2-aminopropane) hydrochloride (AAPH) and FeSO4/H2O2, in red blood cell ghost membranes and in egg phosphatidylcholine (PC) vesicles. Lipid peroxidation was monitored by membrane fluidity changes assessed by electron paramagnetic resonance spectroscopy of a spin-labeled lipid and by the formation of thiobarbituric acid-reactive substances. When lipoperoxidation was initiated by the hydroxyl radical in erythrocyte ghost membranes, both 4-NC and α-tocopherol acted in a very efficient manner. However, lower activities were observed when lipoperoxidation was initiated by the peroxyl radical; and, in this case, the protective effect of α-tocopherol was lower than that of 4-NC. In egg PC vesicles, malondialdehyde formation indicated that 4-NC was effective against lipoperoxidation initiated by both AAPH and FeSO4/H2O2, whereas α-tocopherol was less efficient in protecting against lipoperoxidation by AAPH, and behaved as a pro-oxidant for FeSO4/H2O2. The DPPH (2,2-diphenyl-1-picrylhydrazyl) free-radical assay indicated that two free radicals were scavenged per 4-NC molecule, and one free radical was scavenged per α-tocopherol molecule. These data provide new insights into the antioxidant capacity of 4-NC, which may have therapeutic applications for formulations designed to protect the skin from sunlight irradiation.


Asunto(s)
Antioxidantes/farmacología , Catecoles/farmacología , Membrana Eritrocítica/efectos de los fármacos , Peróxidos/análisis , Fosfolípidos/farmacología , alfa-Tocoferol/farmacología , Amidinas/administración & dosificación , Amidinas/farmacología , Espectroscopía de Resonancia por Spin del Electrón , Radicales Libres/análisis , Humanos , Peroxidación de Lípido/efectos de los fármacos , Malondialdehído/análisis , Fosfatidilcolinas/farmacología , Extractos Vegetales/química , Extractos Vegetales/farmacología , Raíces de Plantas/química
2.
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;46(9): 780-788, 19/set. 2013. graf
Artículo en Inglés | LILACS | ID: lil-686573

RESUMEN

4-Nerolidylcatechol (4-NC) is found in Pothomorphe umbellata root extracts and is reported to have a topical protective effect against UVB radiation-induced skin damage, toxicity in melanoma cell lines, and antimalarial activity. We report a comparative study of the antioxidant activity of 4-NC and α-tocopherol against lipid peroxidation initiated by two free radical-generating systems: 2,2′-azobis(2-aminopropane) hydrochloride (AAPH) and FeSO4/H2O2, in red blood cell ghost membranes and in egg phosphatidylcholine (PC) vesicles. Lipid peroxidation was monitored by membrane fluidity changes assessed by electron paramagnetic resonance spectroscopy of a spin-labeled lipid and by the formation of thiobarbituric acid-reactive substances. When lipoperoxidation was initiated by the hydroxyl radical in erythrocyte ghost membranes, both 4-NC and α-tocopherol acted in a very efficient manner. However, lower activities were observed when lipoperoxidation was initiated by the peroxyl radical; and, in this case, the protective effect of α-tocopherol was lower than that of 4-NC. In egg PC vesicles, malondialdehyde formation indicated that 4-NC was effective against lipoperoxidation initiated by both AAPH and FeSO4/H2O2, whereas α-tocopherol was less efficient in protecting against lipoperoxidation by AAPH, and behaved as a pro-oxidant for FeSO4/H2O2. The DPPH (2,2-diphenyl-1-picrylhydrazyl) free-radical assay indicated that two free radicals were scavenged per 4-NC molecule, and one free radical was scavenged per α-tocopherol molecule. These data provide new insights into the antioxidant capacity of 4-NC, which may have therapeutic applications for formulations designed to protect the skin from sunlight irradiation.


Asunto(s)
Humanos , Antioxidantes/farmacología , Catecoles/farmacología , Membrana Eritrocítica/efectos de los fármacos , Peróxidos/análisis , Fosfolípidos/farmacología , alfa-Tocoferol/farmacología , Amidinas/administración & dosificación , Amidinas/farmacología , Espectroscopía de Resonancia por Spin del Electrón , Radicales Libres/análisis , Peroxidación de Lípido/efectos de los fármacos , Malondialdehído/análisis , Fosfatidilcolinas/farmacología , Extractos Vegetales/química , Extractos Vegetales/farmacología , Raíces de Plantas/química
3.
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;45(6): 473-481, June 2012. ilus, tab
Artículo en Inglés | LILACS | ID: lil-622783

RESUMEN

Electron paramagnetic resonance (EPR) spectroscopy of spin labels was used to monitor membrane dynamic changes in erythrocytes subjected to oxidative stress with hydrogen peroxide (H2O2). The lipid spin label, 5-doxyl stearic acid, responded to dramatic reductions in membrane fluidity, which was correlated with increases in the protein content of the membrane. Membrane rigidity, associated with the binding of hemoglobin (Hb) to the erythrocyte membrane, was also indicated by a spin-labeled maleimide, 5-MSL, covalently bound to the sulfhydryl groups of membrane proteins. At 2% hematocrit, these alterations in membrane occurred at very low concentrations of H2O2 (50 µM) after only 5 min of incubation at 37°C in azide phosphate buffer, pH 7.4. Lipid peroxidation, suggested by oxidative hemolysis and malondialdehyde formation, started at 300 µM H2O2 (for incubation of 3 h), which is a concentration about six times higher than those detected with the probes. Ascorbic acid and α-tocopherol protected the membrane against lipoperoxidation, but did not prevent the binding of proteins to the erythrocyte membrane. Moreover, the antioxidant (+)-catechin, which also failed to prevent the cross-linking of cytoskeletal proteins with Hb, was very effective in protecting erythrocyte ghosts from lipid peroxidation induced by the Fenton reaction. This study also showed that EPR spectroscopy can be useful to assess the molecular dynamics of red blood cell membranes in both the lipid and protein domains and examine oxidation processes in a system that is so vulnerable to oxidation.


Asunto(s)
Humanos , Antioxidantes/farmacología , Membrana Eritrocítica/efectos de los fármacos , Peróxido de Hidrógeno/farmacología , Peroxidación de Lípido/efectos de los fármacos , Proteínas de la Membrana/metabolismo , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo , Ácido Ascórbico/farmacología , Catequina/farmacología , Óxidos N-Cíclicos/metabolismo , Espectroscopía de Resonancia por Spin del Electrón , Membrana Eritrocítica/química , Membrana Eritrocítica/fisiología , Hemólisis , Concentración de Iones de Hidrógeno , Hemoglobinas/metabolismo , Peróxido de Hidrógeno/metabolismo , Fluidez de la Membrana/efectos de los fármacos , Estrés Oxidativo/fisiología , alfa-Tocoferol/farmacología
4.
Braz J Med Biol Res ; 45(6): 473-81, 2012 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-22473321

RESUMEN

Electron paramagnetic resonance (EPR) spectroscopy of spin labels was used to monitor membrane dynamic changes in erythrocytes subjected to oxidative stress with hydrogen peroxide (H(2)O(2)). The lipid spin label, 5-doxyl stearic acid, responded to dramatic reductions in membrane fluidity, which was correlated with increases in the protein content of the membrane. Membrane rigidity, associated with the binding of hemoglobin (Hb) to the erythrocyte membrane, was also indicated by a spin-labeled maleimide, 5-MSL, covalently bound to the sulfhydryl groups of membrane proteins. At 2% hematocrit, these alterations in membrane occurred at very low concentrations of H(2)O(2) (50 µM) after only 5 min of incubation at 37°C in azide phosphate buffer, pH 7.4. Lipid peroxidation, suggested by oxidative hemolysis and malondialdehyde formation, started at 300 µM H(2)O(2) (for incubation of 3 h), which is a concentration about six times higher than those detected with the probes. Ascorbic acid and α-tocopherol protected the membrane against lipoperoxidation, but did not prevent the binding of proteins to the erythrocyte membrane. Moreover, the antioxidant (+)-catechin, which also failed to prevent the cross-linking of cytoskeletal proteins with Hb, was very effective in protecting erythrocyte ghosts from lipid peroxidation induced by the Fenton reaction. This study also showed that EPR spectroscopy can be useful to assess the molecular dynamics of red blood cell membranes in both the lipid and protein domains and examine oxidation processes in a system that is so vulnerable to oxidation.


Asunto(s)
Antioxidantes/farmacología , Membrana Eritrocítica/efectos de los fármacos , Peróxido de Hidrógeno/farmacología , Peroxidación de Lípido/efectos de los fármacos , Proteínas de la Membrana/metabolismo , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo , Ácido Ascórbico/farmacología , Catequina/farmacología , Óxidos N-Cíclicos/metabolismo , Espectroscopía de Resonancia por Spin del Electrón , Membrana Eritrocítica/química , Membrana Eritrocítica/fisiología , Hemoglobinas/metabolismo , Hemólisis , Humanos , Peróxido de Hidrógeno/metabolismo , Concentración de Iones de Hidrógeno , Fluidez de la Membrana/efectos de los fármacos , Estrés Oxidativo/fisiología , alfa-Tocoferol/farmacología
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