Identification and mapping of groundwater recharge zones using multi influencing factor and analytical hierarchy process.

The management of groundwater systems is essential for nations that rely on groundwater as the principal source of communal water supply (e.g., Mohmand District of Pakistan). The work employed Remote Sensing and GIS datasets to ascertain the groundwater recharge zones (GWRZ) in the Mohmand District of Pakistan. Subsequently, a sensitivity analysis was conducted to examine the impact of geology and hydrologic factors on the variability of the GWRZ. The GWRZ was determined by employing weighted overlay analysis on thematic maps derived from datasets about drainage density, slope, geology, rainfall, lineament density, land use/land cover, and soil types. The use of multi-criteria decision analysis (MCDA) involves the utilization of the multi-influencing factor (MIF) and analytical hierarchy procedure (AHP) to allocate weights to the selected influencing factors. The MIF data found that very high groundwater recharge spanned 1.20%, high zones covered 40.44%, moderate zones covered 50.81%, and low zones covered 7.54%. In comparison, the AHP technique results suggest that 1.81% of the whole area is very high, 33.26 is high, 55.01% is moderate, and 9.92% has low groundwater potential. The geospatial-assisted multi-influencing factor approach helps increase conceptual knowledge of groundwater resources and evaluate possible groundwater zones.

SHORT-ROOT stabilizes PHOSPHATE1 to regulate phosphate allocation in Arabidopsis.

The coordinated distribution of inorganic phosphate (Pi) between roots and shoots is an important process that plants use to maintain Pi homeostasis. SHORT-ROOT (SHR) is well characterized for its function in root radial patterning. Here we demonstrate a role of SHR in controlling Pi allocation from root to shoot by regulating PHOSPHATE1 in the root differentiation zone. We recovered a weak mutant allele of SHR in Arabidopsis that accumulates much less Pi in the shoot and shows a constitutive Pi starvation response under Pi-sufficient conditions. In addition, Pi starvation suppresses SHR protein accumulation and releases its inhibition on the HD-ZIP III transcription factor PHB. PHB accumulates and directly binds the promoter of PHOSPHATE2 to upregulate its transcription, resulting in PHOSPHATE1 degradation in the xylem-pole pericycle cells. Our findings reveal a previously unrecognized mechanism of how plants regulate Pi translocation from roots to shoots.

An Agrobacterium-mediated transient expression method contributes to functional analysis of a transcription factor and potential application of gene editing in Chenopodium quinoa.

KEY MESSAGE: An efficient Agrobacterium-mediated transient expression method was developed, which contributed to the functional characterization of the transcription factor CqPHR1, and demonstrates the potential application of gene editing in quinoa. Chenopodium quinoa is a crop expected to ensure global food security in future due to its high resistance to multiple abiotic stresses and nutritional value. We cloned one of the paralogous genes of the Arabidopsis homolog PHR1 (PHOSPHATE STARVATION RESPONSE 1) in quinoa-inbred lines by reverse genetic approach. Overexpression of CqPHR1 driven by the constitutive CaMV 35S promoter in Arabidopsis phr1 mutant can complement its phenotypes, including the induction of phosphate starvation-induced (PSI) genes and anthocyanin accumulation in leaves. By Agrobacterium-mediated gene transient expression, we found that CqPHR1 localized in the nucleus of quinoa cells, and overexpression of CqPHR1 in quinoa cells promoted PSI genes expression, which further revealed the function of CqPHR1 as a transcription factor. We have also shown that the transient expression system can be used to express Cas9 protein in various quinoa-inbred lines and perform effective gene editing in quinoa tissue. The method developed in this study will be useful for verifying the effectiveness of gene-editing systems in quinoa cells and has potential application in the generation of gene-edited quinoa with heritable traits.

WIPK-NtLTP4 pathway confers resistance to Ralstonia solanacearum in tobacco.

KEY MESSAGE: WIPK-NtLTP4 module improves the resistance to R. solanacearum via upregulating the expression of defense-related genes, increasing the antioxidant enzyme activity, and promoting stomatal closure in tobacco. Lipid transfer proteins (LTPs) are a class of small lipid binding proteins that play important roles in biotic and abiotic stresses. The previous study revealed that NtLTP4 positively regulates salt and drought stresses in Nicotiana tabacum. However, the role of NtLTP4 in biotic stress, especially regarding its function in disease resistance remains unclear. Here, the critical role of NtLTP4 in regulating resistance to Ralstonia solanacearum (R. solanacearum), a causal agent of bacterial wilt disease in tobacco, was reported. The NtLTP4-overexpressing lines markedly improved the resistance to R. solanacearum by upregulating the expression of defense-related genes, increasing the antioxidant enzyme activity, and promoting stomatal closure. Moreover, NtLTP4 interacted with wound-induced protein kinase (WIPK; a homolog of MAPK3 in tobacco) and acted in a genetically epistatic manner to WIPK in planta. WIPK could directly phosphorylate NtLTP4 to positively regulate its protein abundance. Taken together, these results broaden the knowledge about the functions of the WIPK-NtLTP4 module in disease resistance and may provide valuable information for improving tobacco plant tolerance to R. solanacearum.

Lewis Base-Catalyzed [4 + 3] Annulation of ortho-Quinone Methides and MBH Carbonates: Synthesis of Functionalized Benzo[ b]oxepines Bearing Oxindole Scaffolds.

A novel Lewis base-catalyzed [4 + 3] annulation process for the construction of benzo[ b]oxepine scaffolds has been developed. 1,4-Diazabicyclo[2.2.2]octane (DABCO) promotes the union of o-QMs and Morita-Baylis-Hillman carbonates in reasonable to excellent yields and good stereoselectivities (dr > 20:1). This straightforward, catalytic approach offers access to a variety of synthetically useful benzo[ b]oxepine derivatives bearing oxindole scaffolds containing all-carbon spiro-quaternary stereocenters.

Harmful effect of nanoparticles on the functions of freshwater ecosystems: Insight into nanoZnO-polluted stream.

ZnO nanoparticle toxicity on aquatic organisms has been extensively studied, but its concentration-and time-dependent effects on ecosystem functioning are remain uncertain. Here we assessed the harmful effects of nano-ZnO (10, 100, 1000 mg L-1) on the stream functioning by using a microcosm system simulating poplar leaf decomposition for 50 days. The 100 mg L-1 ZnO nanoparticles had significantly and stably inhibitory effect on the litter decomposition during the exposure period. The inhibition was not detected in the 10 mg L-1 treatment until 43 d. In contrast, the significant and continuous inhibition started to disappear from 43 d in the 1000 mg L-1 treatment. The varied consequences on litter decomposition might be directly affected by the different ZnO nanoparticle homogeneity of the different treatments. ZnO nanoparticles led to significant decreases in pH value of the decomposition environment, which had significant and positive relationships to the activities of dehydrogenase, glycine-aminopeptidase, N-acetylglucosaminidase, and acid phosphatase. Besides, 10 and 1000 mg L-1 ZnO nanoparticles led to lower fungal diversity, which was negatively related to the variability of decomposition. In conclusion, fungal decomposers showed different responses to the different concentrations of ZnO nanoparticle, and ultimately affected the stability of ecosystem functions.

Hepatotoxicity and immunotoxicity of MC-LR on silver carp.

Microcystins produced by some cyanobacteria can cause damages to the liver and kidneys of aquatic animals. In the natural water with cyanobacterial blooms, silver carp may suffer from the most serious affect of the bloom due to their filtering these cyanobacteria and ingesting them as food. In the present study, silver carp was exposed to microcystin-LR by using the method of intraperitoneal injection first to determine the acute toxicity of microcystin-LR on silver carp and then to determine the activity of inflammatory protein and content of inflammatory factors from the serum of silver carp following a subacute exposure of microcystin-LR at doses of 104.9 µg kg-1 (1/5 of LD50) or 262.1 µg kg-1 (1/2 of LD50). The results showed that MC-LR exposure increased fish liver index and promoted the activities of fish serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), indicating the hepatotoxicity of MC-LR on the fish. Moreover, MC-LR exposure also increased the number of leukocytes, complement C3 level, lysozyme activity (at the first 9 h of exposure), and the contents of cytokines TNF-α, IL-1ß and IFN-γ in fish serum. In addition, a significant increase in IgM level was observed in the serum and head kidney of silver carp following MC-LR exposure. This result suggests that semi-lethal doses of MC-LR exposure is not only hepatotoxic but also immunotoxic to silver carp.

Lewis Acid Catalyzed Tandem 1,4-Conjugate Addition/Cyclization of in Situ Generated Alkynyl o-Quinone Methides and Electron-Rich Phenols: Synthesis of Dioxabicyclo[3.3.1]nonane Skeletons.

A versatile Lewis acid catalyzed tandem cyclization of in situ generated alkynyl o-quinone methides ( o-AQMs) with electron-rich phenols has been developed on the basis of the mode involving an intermolecular 1,4-conjugate addition/6-endo cyclization/1,3-aryl shift/intramolecular 1,4-conjugate addition cascade. This reaction provides a new method for expeditious assembly of synthetically and biologically interesting tetracyclic bridged dioxabicyclo[3.3.1]nonane skeletons featuring a congested bridgehead oxa-quaternary stereocenter.