RESUMEN
The maturation of mitochondrial RNA transcripts proceeds through several steps. Here we use insects trypanosomatid Leptomonas seymouri as a model organism for analysis of transcription and posttranscriptional processing of mitochondria encoded gene for the subunit 6 of ATPase (A6). It was shown that Cyt b (cytochrome b) and A6 genes were transcribed and edited as a polycistronic template. Analysis of twelve RT-PCR products of both genes led to identification of four types of differently and/or partially edited cDNA molecules. Based on the analysis of two fully edited A6 transcripts we propose the existence of two alternatively edited products.
Asunto(s)
Adenosina Trifosfatasas/genética , Edición de ARN , ARN Mensajero/genética , ARN Protozoario/genética , ARN/genética , Trypanosomatina/genética , Animales , Secuencia de Bases , Citocromos b/genética , ADN Protozoario/genética , Mitocondrias/genética , Datos de Secuencia Molecular , Subunidades de Proteína/genética , ARN Mitocondrial , Transcripción GenéticaRESUMEN
The fragment of kinetoplast maxicircle (mitochondrial) DNA divergent region Leptomonas seymouri was localized containing promotor of 12S rRNA gene. The ability of this fragment to initiate transcription was tested with transcription system in organello in isolated mitochondria of L. seymouri.
Asunto(s)
ADN Mitocondrial/genética , Regiones Promotoras Genéticas , ARN Ribosómico/genética , Trypanosomatina/genética , Animales , Secuencia de Bases , Cartilla de ADN , Electroforesis en Gel de PoliacrilamidaRESUMEN
The sequence of mitochondrial A6 (MURF4) was compared for several trypanosomatids in order to assess the reduction of the edited domain (ED). The association between the ED reduction and the phylogenetic position of a species proved to be less tight than believed earlier. Compared with digenetic species, monogenetic ones more often displayed ED reduction and had smaller ED.
