Ameliorating effects of Nigella sativa oil on aggravation of inflammation, oxidative stress and cytotoxicity induced by smokeless tobacco extract in an allergic asthma model in Wistar rats

BACKGROUND: The comparison of smokeless tobacco (ST) exposure versus Ovalbumin (Ova) sensitized rats or asthmatic patients has hardly been studied in the literature. Thus, the present study aims to investigate the aggravation of inflammation, exacerbation of asthma, oxidative stress and cytotoxicity induced by ST. METHODS: ST was given at the dose of 40 mg/kg in an allergic asthma model in Wistar rats. Furthermore, the effects of oral administration of Nigella sativa oil (NSO), at a dose of 4 mL/kg/day, were investigated. RESULTS: The obtained results showed that ST clearly enhanced lung inflammation through interleukin-4 (IL-4) and Nitric oxide (NO) increased production. Actually, ST was found to intensify the oxidative stress state induced by Ova-challenge in rats, which was proven not only by augmenting lipid peroxidation and protein oxidation, but also by altering the non-enzymatic and enzymatic antioxidant status. Furthermore, the aggravation of inflammation and oxidative stress was obviously demonstrated by the histopathological changes observed in lung. In contrast, NSO administration has shown anti-inflammatory effects by reducing IL-4 and NO production, restoring the antioxidant status, reducing lipid peroxidation and improving the histopathological alterations by both protein oxidation and NSO treatment. CONCLUSIONS: Our data have proven that severe concurrent exposure to allergen and ST increases airway inflammation and oxidative stress in previously sensitized rats. They also suggest that the oral NSO treatment could be a promising treatment for asthma

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Ameliorating effects of Nigella sativa oil on aggravation of inflammation, oxidative stress and cytotoxicity induced by smokeless tobacco extract in an allergic asthma model in Wistar rats.

BACKGROUND: The comparison of smokeless tobacco (ST) exposure versus Ovalbumin (Ova) sensitized rats or asthmatic patients has hardly been studied in the literature. Thus, the present study aims to investigate the aggravation of inflammation, exacerbation of asthma, oxidative stress and cytotoxicity induced by ST. METHODS: ST was given at the dose of 40mg/kg in an allergic asthma model in Wistar rats. Furthermore, the effects of oral administration of Nigella sativa oil (NSO), at a dose of 4mL/kg/day, were investigated. RESULTS: The obtained results showed that ST clearly enhanced lung inflammation through interleukin-4 (IL-4) and Nitric oxide (NO) increased production. Actually, ST was found to intensify the oxidative stress state induced by Ova-challenge in rats, which was proven not only by augmenting lipid peroxidation and protein oxidation, but also by altering the non-enzymatic and enzymatic antioxidant status. Furthermore, the aggravation of inflammation and oxidative stress was obviously demonstrated by the histopathological changes observed in lung. In contrast, NSO administration has shown anti-inflammatory effects by reducing IL-4 and NO production, restoring the antioxidant status, reducing lipid peroxidation and improving the histopathological alterations by both protein oxidation and NSO treatment. CONCLUSIONS: Our data have proven that severe concurrent exposure to allergen and ST increases airway inflammation and oxidative stress in previously sensitized rats. They also suggest that the oral NSO treatment could be a promising treatment for asthma.

Eosinophilic activity and bronchial hyperresponsiveness within an asthmatic paediatric population

Background: Our study aims to assess the importance of serum eosinophil cationic protein (ECP) levels as a non-invasive marker of bronchial hyperresponsiveness (BHR) in children with asthma, and may predict objectively the asthmatic severity and sensitivities. Methods: This study, which was carried out on 75 asthmatic patients from a paediatric population (average age: nine years old, sex-ratio M/F: 1.64), is based on both interrogation conducted by the clinician and biological explorations, essentially serological testing of ECP and eosinophilia determination, as well as the measurement of serological IgE amounts. Results: The analysis of the questionnaires and the biological results allowed us to evaluate the clinico-biological relations within this population. ECP, more than eosinophilia, proves to be a relevant marker of asthma severity (p<0.05) and sensitivities within this given population (r=0.65). Conclusion: We were able to show that the evaluation of the serological levels of ECP seems to be a good biological marker of asthma(AU)

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Eosinophilic activity and bronchial hyperresponsiveness within an asthmatic paediatric population.

BACKGROUND: Our study aims to assess the importance of serum eosinophil cationic protein (ECP) levels as a non-invasive marker of bronchial hyperresponsiveness (BHR) in children with asthma, and may predict objectively the asthmatic severity and sensitivities. METHODS: This study, which was carried out on 75 asthmatic patients from a paediatric population (average age: nine years old, sex-ratio M/F: 1.64), is based on both interrogation conducted by the clinician and biological explorations, essentially serological testing of ECP and eosinophilia determination, as well as the measurement of serological IgE amounts. RESULTS: The analysis of the questionnaires and the biological results allowed us to evaluate the clinico-biological relations within this population. ECP, more than eosinophilia, proves to be a relevant marker of asthma severity (p<0.05) and sensitivities within this given population (r=0.65). CONCLUSION: We were able to show that the evaluation of the serological levels of ECP seems to be a good biological marker of asthma.