RESUMEN
A series of star poly(epsilon-caprolactone)s (PCL) with dendritic cores, PAMAM-PCLs, were synthesized through the ring-opening polymerization of epsilon-caprolactone (CL) initiated by poly(amidoamine) dendrimer (PAMAM-OH). By controlling the feed ratio of the macroinitiator PAMAM-OH to the monomer CL, the star polymers with different branch lengths and properties can be obtained. The successful incorporation of PCL sequences onto the PAMAM-OH core was verified by FTIR, 1H NMR, and combined size-exclusion chromatography and multiangle laser light scattering analysis. The in vitro degradation of PAMAM-PCLs was investigated. The results show the hydrolytic degradation rate increases with increasing content of hydrophilic PAMAM-OH core. While the enzymatic degradation rate is affected by two competitive factors, the catalytic effect of Pseudomonas cepacia lipase on the degradation of PCL branches and the hydrophilicity that depends on the polymer composition. Using the PAMAM-PCLs with different molecular weights, the microsphere drug delivery systems with submicron sizes were fabricated using an "ultrasonic assisted precipitation method." The in vitro drug release from these microspheres was investigated.
Asunto(s)
Materiales Biocompatibles/química , Portadores de Fármacos/química , Burkholderia cepacia/enzimología , Dendrímeros/química , Hidrólisis , Lipasa/química , Espectroscopía de Resonancia Magnética , Poliésteres/química , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Biodegradable amphiphilic graft copolymers poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide]-g-poly(epsilon-caprolactone) (PHEA-g-PCL) with different branch lengths were synthesized through the ring-opening polymerization of epsilon-caprolactone initiated by the macroinitiator PHEA bearing hydroxyl groups. With use of the graft copolymers with different compositions, nanoparticle drug delivery systems with sizes smaller than 100 nm were prepared by a dialysis method, and microparticle drug delivery systems with sizes smaller than 5 microm were fabricated by a melting-emulsion method. The regularly spherical shapes of the drug-loaded nano- and microparticles were verified by transmission electron microscopy and scanning electron microscopy. In vitro drug release properties of nano- and microparticle drug delivery systems were investigated, with the emphasis on the effects of polymer composition, particle size, and drug-loading content on the release behaviors.
Asunto(s)
Lactonas/química , Lactonas/síntesis química , Nanoestructuras/química , Polímeros/química , Polímeros/síntesis química , Diálisis , Sistemas de Liberación de Medicamentos , Estructura Molecular , Tamaño de la Partícula , Factores de TiempoRESUMEN
A series of biodegradable amphiphilic graft polymers were successfully synthesized by grafting poly(epsilon-caprolactone) (PCL) sequences onto a water-soluble poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide] (PHEA) backbone. The graft copolymers were prepared through the ring-opening polymerization of epsilon-caprolactone (CL) initiated by the macroinitiator PHEA with pendant hydroxyl groups without adding any catalyst. By controlling the feed ratio of the macroinitiator to the monomer, the copolymers with different branch lengths and properties can be obtained. The successful grafting of PCL sequences onto the PHEA backbone was verified by FTIR, 1H NMR, and combined size-exclusion chromatography and multiangle laser light scattering (SEC-MALLS) analysis. The hydrolytic degradation and enzymatic degradation of these graft copolymers were investigated. The results show the hydrolytic degradation rate increases with increasing content of hydrophilic PHEA backbone. While the enzymatic degradation rate is affected by two competitive factors, the catalytic effect of Pseudomonas cepacia lipase on the degradation of PCL branches and the hydrophilicity which depends on the copolymer composition. In situ observation of the degradation under polarizing light microscope (PLM) demonstrates the different degradation rates of different regions in the polymer samples.