RESUMEN
The purpose of this study is the assessment of the acute and chronic toxicity of pharmacological substance sodium, calcium, iron-polygalacturonate (PG Na,Ca,Fe) in rabbits as one of the stages of preclinical studies. We studied an acute and chronic oral toxicity of PG Na,Ca,Fe, which stimulates the process of hemopoiesis, in male and female rabbits of the "Chinchilla". According to the results of the study of acute toxicity of PG Na,Ca,Fe, treating with it the rabbits of both sexes in doses of 0.5-5â¯g/kg has no toxic effect (LD50 greater than 5â¯g/kg). The histostructure of studied organs of animals, treated with preparations in a dose of 5â¯g/kg, did not differ from that of the animals of the control group. This study allow to classify PG Na,Ca,Fe as a preparation of the 6th class with respect to harmless drugs. An estimate of the chronic toxicity of PG Na,Ca,Fe at administration of preparation in the form of boluses to rabbits in doses 0.025, 0.262 and 0.5â¯g/kg of the body weight demonstrated that the general condition and behavior of animals did not differ from the norm. The data of hematological and biochemical studies of blood serum and urine, electrocardiographic studies, the study of the mass coefficients of the internal organs of the experimental rabbits, treated with PG Na,Ca,Fe in the mentioned doses for 60â¯days, compared to those obtained in the 30-day post-observation period, did not show significant changes with respect to the control and intact group of rabbits.
RESUMEN
New water-soluble pectin complexes with Ca(2+), Mg(2+), Co(2+), Cu(2+), Fe(2+), Mn(2+), Zn(2+) on the basis of pectin biopolymer have been synthesized and successfully tested on white rats. For a starting, we have obtained a sodium pectate to enhance solubility of target complexes as a whole. Shortly afterwards, running the reaction of ligand exchange of Nа(+) ions with corresponding s-, d- metal cations we were able to synthesize new pectin complexes. The ranges of s-, d-metals salts concentrations were detected experimentally, in which the selective formation of water-soluble complexes occurred. Antianemic effect of new pectin complexes with Na, Fe and Na, Ca, Fe was investigated on white rats with posthemorrhagic anemia. Under the effect of complexes, the improvement of animals and prevention of erythropoiesis disorders were observed. Antianemic effect of the complexes manifested itself in the doses equivalent to 25% or 50% of the iron daily rate, recommended in the treatment of iron-deficiency anemia with the drugs based on iron sulphate.