1.
Nucleosides Nucleotides Nucleic Acids
; 26(10-12): 1265-8, 2007.
Artículo
en Inglés
| MEDLINE
| ID: mdl-18066765
RESUMEN
An artificial restriction enzyme, which we developed recently by combining Ce(IV)/EDTA and peptide nucleic acids, was used for PCR-free construction of a fusion protein. The fusion protein was successfully expressed in mammalian cells. This artificial DNA cutter can be also applied to site-selective scission of huge DNAs. Promising features of this novel tool were concretely evidenced.
Asunto(s)
Cerio/química , División del ADN , Enzimas de Restricción del ADN/química , ADN/química , Ácido Edético/química , Ácidos Nucleicos de Péptidos/química , Animales , Bacteriófago lambda/genética , Secuencia de Bases , Línea Celular , Clonación Molecular , ADN Viral/química , Genoma Viral , Datos de Secuencia Molecular , Proteínas Recombinantes de Fusión/genética
2.
Nucleic Acids Symp Ser (Oxf)
; (50): 265-6, 2006.
Artículo
en Inglés
| MEDLINE
| ID: mdl-17150919
RESUMEN
Genomic DNAs of lambda phage (48.5 kbp) and E. coli (4.6 Mbp) were successfully cleaved at the target site by artificial restriction DNA cutter (ARCUT), which we recently developed by combining Ce(IV)/EDTA and PNA (peptide nucleic acid). PNA satisfactorily invaded the target site in such huge DNAs and activate there for Ce(IV)/EDTA-induced scission.
Asunto(s)
Enzimas de Restricción del ADN/química , Ácido Edético/química , Ácidos Nucleicos de Péptidos/química , Bacteriófago lambda/genética , Cerio/química , ADN Bacteriano/química , ADN Bacteriano/metabolismo , ADN Viral/química , ADN Viral/metabolismo , Escherichia coli/genética , Hidrólisis
3.
Cerebrovasc Dis
; 20(3): 207-8, 2005.
Artículo
en Inglés
| MEDLINE
| ID: mdl-16088118
