Protein tyrosine phosphatase receptor type delta (PTPRD) gene in an animal model of restless legs syndrome.

The pathophysiology of the restless legs syndrome (RLS) is related to dopaminergic dysfunction, reduced iron and variations in gene expression, such as the protein tyrosine phosphatase receptor type delta gene (PTPRD). Animal models could be key to achieving a mechanistic understanding of RLS and to facilitate efficient platforms for evaluating new therapeutics. Thus, the aim of this study was to evaluate the expression of PTPRD, of genes and proteins associated with RLS, the sleep patterns and the cardiovascular parameters in an animal model of RLS (spontaneously hypertensive rat [SHR]). Rats were divided into two groups: (i) Wistar-Kyoto and (ii) SHR. Cardiovascular parameters were assessed by tail plethysmography. Polysomnography was used to analyse the sleep pattern (24 h). For the PTPRD analyses, quantitative polymerase chain reaction (qPCR) and indirect enzyme-linked immunosorbent assay (ELISA) techniques were used. To evaluate the tyrosine hydroxylase enzyme, dopamine transporter (DAT) and type 2 dopaminergic receptor, qPCR and Western Blotting techniques were used. For the quantification of iron, ferritin and transferrin, the ELISA method was used. SHRs had higher blood pressure, alterations in sleep pattern, lower expression of protein content of PTPRD, lower expression of DAT, and lower serum concentrations of ferritin. These data suggest that the behavioural, physiological, and molecular changes observed in SHRs provide a useful animal model of RLS, reinforcing the importance of this strain as an animal model of this sleep disorder.

Efeito de video games ativos sobre a capacidade funcional e o humor de idosos: revisão sistemática e meta-análise / Effect of active video games on functional capacity and mood in older adults: systematic review and meta-analysis

Nos últimos anos, os video games ativos (VGAs) têm sido utilizados pela população geral como uma alternativa para a prática de exercício físico associada ao entretenimento. O objetivo deste estudo foi realizar uma revisão sistemática sobre o efeito dos VGAs na capacidade funcional e no estado de humor de idosos. Para a seleção dos artigos, considerou-se estudos publicados entre 2006 e 2017, realizados com idosos (> 60 anos); que utilizaram como intervenção, exercícios realizados em VGAs Nintendo Wii ou Xbox Kinect. Os resultados observados sugerem melhoras no equilíbrio, na força muscular de membros inferiores e na mobilidade funcional. No perfil de humor, os resultados são controversos. Sendo assim, podemos concluir que o exercício realizado em VGAs pode melhorar a capacidade funcional, no entanto, os benefícios no humor precisam ser mais investigados, especial-mente em estudos de longo prazo.

In the last years, the active video games (AVGs) have been used by the general population as an alternative to exercise and entertainment. The aim of this study was to do a systematic review about the effects of AVGs on the functional capacity and the mood in older adults. We selected studies published between 2006 and 2017, with older adults (> 60 years) and only that had interventions with Nintendo Wii or Xbox Kinect. The results suggested improvements in the balance, muscle strength of lower limbs and functional mobility. In the mood profile, the results are controversial. We could conclude that AVGs exercising may improve functional capacity, however, the benefits in the mood, need further investigations, especially in the long-term studies.