RESUMEN
The article deals with results of studying parahemolytic vibrio separatedfrom different sources according their phenotype and genotype attributes associated with virulence. In certain cases the mismatch of results of Kanagava tests and polymerase chain reaction test of gene tdh was established. The need in virulence complex evaluation is substantiated. This complex has to include detection of hemolytic activity in Kanagava test and urease activity on the Kristensen medium and polymerase chain reaction detection of genes tdh and trh. The developed complex technique is described. The formula of pathogenic strains is established Three alternatives of virulent parahemolytic vibrio are given. The test-strains Vibrio parahaemolyticus are proposed as control in testing phenotype and genotype strains according virulence signs.
Asunto(s)
Proteínas Bacterianas/genética , Proteínas Hemolisinas/genética , Tipificación Molecular/métodos , Vibrio parahaemolyticus/patogenicidad , Toxinas Bacterianas/genética , Reacción en Cadena de la Polimerasa , Serotipificación , Vibrio parahaemolyticus/clasificación , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/aislamiento & purificación , Virulencia , Factores de Virulencia/genéticaRESUMEN
V. parahaemolyticus and V. alginolyticus strains isolated from patients during an outbreak of an acute enteric disease in Vladivostok in 1997 were studied. All strains were found to possess typical taxonomic signs. V. parahaemolyticus isolated from humans had direct heat stable haemolysin exotoxin. The overwhelming majority of these strains belonged to serovar O3K6. Among the cultures under study 7 phage types were determined: phage types 1, 2, 7, 10 in 8 V. parahaemolyticus strains and phage types 2, 4. 5. 7 in 5 V. alginolyticus strains. The diagnostic halophilic phage lyzed vibrios in 30.2% of strains. The cultures under study were found to be highly sensitive to chloramphenicol, cefotaxime, nalidixic acid and cyprofloxacin. The study proved that the outbreak of alimentary toxicoinfection was caused by vibrios of serogroup O3:K6.
Asunto(s)
Brotes de Enfermedades , Enfermedades Transmitidas por los Alimentos/epidemiología , Vibriosis/epidemiología , Vibrio , Antiinfecciosos/farmacología , Proteínas Bacterianas/análisis , Proteínas Bacterianas/metabolismo , Bacteriólisis , Tipificación de Bacteriófagos , Cefotaxima/farmacología , Cefalosporinas/farmacología , Cloranfenicol/farmacología , Ciprofloxacina/farmacología , Enterotoxinas/análisis , Enterotoxinas/metabolismo , Enfermedades Transmitidas por los Alimentos/microbiología , Humanos , Pruebas de Sensibilidad Microbiana , Ácido Nalidíxico/farmacología , Serotipificación , Siberia/epidemiología , Vibrio/clasificación , Vibrio/efectos de los fármacos , Vibrio/metabolismo , Vibrio/fisiología , Vibriosis/microbiologíaRESUMEN
The materials on the investigation of the outbreak cholera eltor in Vladivostok, caused by the import of infection from China. The leading role of the water route of transmission of this infection is shown due to the contamination of water sources with non-decontaminated sewage water. The complex of antiepidemic measures was carried out, which made it possible to arrest the spread of cholera and liquidate its foci.
