RESUMEN
Serologic and molecular biologic techniques were used to identify association of HTLV-1 with adult T-cell leukemia/lymphoma as well as with B-cell leukemia. HTLV-1 markers (antibodies and integrated provirus) were identified in all lympho- and myeloproliferative diseases, while integrated provirus genome--in T-cell population only.
Asunto(s)
Virus Linfotrópico T Tipo 1 Humano , Leucemia Linfocítica Crónica de Células B/diagnóstico , Leucemia-Linfoma de Células T del Adulto/diagnóstico , Provirus , Diagnóstico Diferencial , Anticuerpos Anti-HTLV-I/análisis , Humanos , Trastornos Mieloproliferativos/diagnóstico , Preleucemia/diagnósticoRESUMEN
The allogenic tumour extracts were studied as immunogens for the prevention of cattle infection with BLV. Immunization did not induce both the production of antibodies against the virus and spontaneous viral infection. Experimental infection of immunized animals with living leukocytes of a leukemic donor caused a BLV infection in 100% of cases.
Asunto(s)
Enfermedades de los Bovinos/prevención & control , Leucemia Experimental/prevención & control , Extractos de Tejidos/inmunología , Animales , Anticuerpos Antineoplásicos/análisis , Anticuerpos Antivirales/análisis , Bovinos , Enfermedades de los Bovinos/etiología , Enfermedades de los Bovinos/inmunología , Pruebas Inmunológicas de Citotoxicidad , Inmunización/métodos , Virus de la Leucemia Bovina/inmunología , Virus de la Leucemia Bovina/patogenicidad , Leucemia Experimental/etiología , Leucemia Experimental/inmunología , Transfusión de Leucocitos , Ganglios Linfáticos/inmunología , Trasplante de Neoplasias , Extractos de Tejidos/administración & dosificación , Trasplante Homólogo , Proteínas del Envoltorio Viral/inmunologíaRESUMEN
The electron microscopic examinations of the short-term cultures of mononuclear cells from patients with T-cell acute lymphoblastic leukemia were conducted for the detection of viral particles. The viral particles which correspond in their morphology to C type retroviruses are observed in the intercellular space of the culture.
Asunto(s)
Cuerpos de Inclusión Viral/ultraestructura , Leucemia Linfoide/sangre , Linfocitos T/ultraestructura , Anticuerpos Antivirales/análisis , Deltaretrovirus/inmunología , Humanos , Cariotipificación , Leucemia Linfoide/inmunología , Leucemia Linfoide/microbiología , Microscopía Electrónica , Linfocitos T/microbiología , Células Tumorales CultivadasRESUMEN
The leukemogenic properties of bovine leukemia virus (BLV), isolated from blood plasma, milk and short-term cultures of blood-circulating leukocytes were studied. A complex life-time examination of 14 calves inoculated with a preparation of BLV established leukemia in 4 animals; it was suspected in 3 calves. By the present time leukemia has been morphologically established in 6 out of 13 calves and one was a suspect. Positive results point directly to the viral etiology of bovine leukemia.