Single molecule investigation of the onset and minimum size of the calcium-mediated junction zone in alginate.

One of the principal roles of alginate, both natively and in commercial applications, is gelation via Ca(2+)-mediated crosslinks between blocks of guluronic acid. In this work, single molecule measurements were carried out between well-characterised series of nearly monodisperse guluronic acid blocks ('oligoGs') using dynamic force spectroscopy. The measurements provide evidence that for interaction times on the order of tens of milliseconds the maximum crosslink strength is achieved by pairs of oligoGs long enough to allow the coordination of 4Ca(2+) ions, with both shorter and longer oligomers forming weaker links. Extending the interaction time from tens to hundreds of milliseconds allows longer oligoGs to achieve much stronger crosslinks but does not change the strength of individual links between shorter oligoGs. These results are considered in light of extant models for the onset of cooperative crosslinking in polyelectrolytes and an anisotropic distribution of oligoGs on interacting surfaces and provide a timescale for the formation and relaxation of alginate gels at the single crosslink level.

Avaliação da partida e operação de filtros anaeróbios tendo bambu como material de recheio / Start up evaluation and operation of anaerobic filters filled up with bamboo

Neste trabalho, foram constituídos três filtros anaeróbios com volume de 500 L preenchidos com anéis de bambu, operando-os com tempo de detenção hidráulica de nove horas. O objetivo foi a determinação do período de partida sem o emprego de inóculo adaptado e a obtenção da eficiência no tratamento de esgoto doméstico, empregando-se um tempo de detenção hidráulica inferior às 12 horas sugeridas pela NBR 7229. Na avaliação do sistema, fez-se uso do carbono orgânico dissolvido, da demanda química de oxigênio, da demanda bioquímica de oxigênio, pH e alcalinidade, evidenciando quanto ao carbono orgânico dissolvido, a necessidade de 19 semanas para que se alcançasse o equilíbrio dinâmico nos reatores. Após esse período, a remoção atingiu 81,4 ± 6,4 por cento. Os resultados demonstraram que o uso de um recheio alternativo nos filtros anaeróbios possibilitou uma eficiência similar a encontrada com materiais tradicionais, mesmo adotando-se um tempo de detenção hidráulica inferior ao indicado pela norma brasileira.

In this paper, three anaerobic filters with 500 L volume were constructed and filled up with bamboo rings, operating with a nine hour hydraulic retention time. The aim was to determinate the start up period without using adapted sludge and reaching efficiency in a wastewater treatment, by deploying hydraulic detention time lower than the 12 hours suggested by the NBR 7229. In the system evaluation, it was used the dissolved organic carbon, chemical oxygen demand, biochemical oxygen demand, pH, and alkalinity, showing in terms of dissolved organic carbon, the need of 19 weeks to reach dynamic equilibrium in the reactors. After this period, the removal achieved 81.4 ± 6.4 percent. Results showed that using an alternative package in anaerobic filters made possible to have an efficacy similar to the one reached with traditional materials, even with the hydraulic detention time lower than the one suggested by the Brazilian law.