The ARKdb: genome databases for farmed and other animals.

The ARKdb genome databases provide comprehensive public repositories for genome mapping data from farmed species and other animals (http://www.thearkdb.org) providing a resource similar in function to that offered by GDB or MGD for human or mouse genome mapping data, respectively. Because we have attempted to build a generic mapping database, the system has wide utility, particularly for those species for which development of a specific resource would be prohibitive. The ARKdb genome database model has been implemented for 10 species to date. These are pig, chicken, sheep, cattle, horse, deer, tilapia, cat, turkey and salmon. Access to the ARKdb databases is effected via the World Wide Web using the ARKdb browser and Anubis map viewer. The information stored includes details of loci, maps, experimental methods and the source references. Links to other information sources such as PubMed and EMBL/GenBank are provided. Responsibility for data entry and curation is shared amongst scientists active in genome research in the species of interest. Mirror sites in the United States are maintained in addition to the central genome server at Roslin.

The effect of preprocedure teaching, relaxation instruction, and music on anxiety as measured by blood pressures in an outpatient gastrointestinal endoscopy laboratory.

A gastrointestinal examination can be frightening to patients. Abnormally elevated blood pressure and pulse caused by anxiety often are present on admission to the hospital. These elevated vital signs are then used as the baseline vital signs for the patient's hospital stay. The first purpose of this study was to determine whether vital signs are elevated because of anxiety on admission to the hospital and how much they decrease after sedation. The second purpose of this study was to explore the effect of instruction about relaxation and the use of music relaxation audio tapes in decreasing patient anxiety. Patients undergoing endoscopic examinations for the first time were randomly assigned into two groups. Patients in the treatment group were given brief instructions in relaxation and provided with an audio tape player and relaxation music. Statistical analysis using multivariate analysis of variance (MANOVA) comparing differences between control and treatment groups over time showed that there was a significant main effect for diastolic blood pressure. Subjects in the treatment group had significantly lower blood pressures throughout the entire GI procedure. There was no significant difference between groups regarding the amount of medication used. Music combined with relaxation is an inexpensive nursing intervention for patients in relaxing during a GI examination.

Ammonium lactate 12% lotion versus a liposome-based moisturizing lotion for plantar xerosis. A double-blind comparison study.

Two emollients, ammonium lactate 12% lotion and a liposome-based moisturizing lotion, were compared in a double-blind test for efficacy in the treatment of plantar xerosis. A total of 43 out of 57 participants (75%) with bilateral plantar xerosis followed instructions completely and applied the lotions (one to each foot) twice daily for 4 weeks. Each participant was evaluated once a week for 6 weeks (the final 2 weeks for evaluation of post-treatment regression) to determine xerotic grade (degree of dryness) and treatment effectiveness. With both lotions, significant improvement began during the second week of treatment and continued into the fourth week. There were no significant differences between the two lotions in the 6-week patterns of either xerotic grade or treatment effectiveness.

Future of bradyarrhythmia therapy systems: automaticity.

Since the first fixed-rate ventricular pacemaker was introduced in the late 1950s, pacing systems have evolved rapidly. Current developments focus on making devices more sophisticated and less complex--a challenging combination. Automaticity features such as beat-by-beat capture verification, sensitivity threshold adaptation, and algorithms to govern dynamically the maximum sensor rate have either recently been introduced or are likely to be introduced in the near future. Technologic advances are likely to allow meaningful improvements in current drain, battery performance, memory capacity, signal processing, telemetry, and programmer interface. Bradyarrhythmia therapy devices of the future promise to go beyond the pacemaker. Ultimately, pacing systems will become part of integrated cardiac rhythm management systems.

Gender differences in physiological reactivity to infant cries and smiles in military families.

OBJECTIVE: The primary purpose of this experiment was to examine gender differences in physiological reactivity to infant cries and smiles in military families. METHOD: Twenty males and 29 females viewed and listened to videotapes of a crying infant and a smiling infant while heart rate, skin resistance, and respiration rate were monitored. All participants were active-duty U.S. Air Force personnel or their spouses. RESULTS: Males showed a larger increase in skin conductance than females during the crying infant stimulus. Males also showed an increase in heart rate during the crying infant stimulus, whereas females did not show any increase in heart rate during the crying infant stimulus. No gender differences in physiological reactivity were obtained during the smiling infant stimulus, although both males and females showed a significant increase in heart rate while viewing the smiling infant. CONCLUSIONS: The results are contrasted with previous reports (e.g., Frodi, Lamb, Leavitt, & Donovan, 1978) of no differences between genders in physiological reactivity to a crying infant. Discussion of the results focuses on models of child physical abuse that involve physiological hyperreactivity. It is hypothesized that the greater physiological reactivity of males than females during a crying infant videotape may partially explain why physical abuse of a child by a male frequently results in more serious damage to the child than physical abuse by a female.

The blood cell transplant program.

OBJECTIVES: To discuss the critical components of a blood cell transplantation (BCT) program as they relate to the standards developed by The Foundation for Accreditation of Hematopoietic Cell Therapy (FAHCT). DATA SOURCES: FAHCT Standards, book chapters, and articles pertaining to developing a BCT program. CONCLUSIONS: BCT is a burgeoning therapy for oncologic and hematologic diseases. New regulations have emerged to promote quality medical and laboratory practice in blood and marrow transplantation. IMPLICATIONS FOR NURSING PRACTICE: As BCT increases for oncologic and hematologic diseases, a growing number of oncology nurses will be caring for BCT recipients and their families. Nurses in administration, research, advance practice, and education are increasingly involved in implementing BCT as a standard therapy for a growing number of diseases.

Struggling to gain meaning: living with the uncertainty of breast cancer.

Uncertainty is a common experience for women living with breast cancer, particularly when treatment cannot assure disease cure. The study described in this article sought to provide insight into uncertainty experiences for women living with breast cancer. Hermeneutic phenomenology and photographic hermeneutics were used to describe and interpret uncertainty for nine women between 2 and 6 years posttreatment for breast cancer. Data were collected using interviews and interpretations of photographs. Five themes of uncertainty among women were uncovered. Major study findings included support for a reconceptualization of Mishel's Uncertainty in Illness Theory and the explication of growth-producing aspects of uncertainty.

Activation and repression of Drosophila alcohol dehydrogenase distal transcription by two steroid hormone receptor superfamily members binding to a common response element.

Developmental activation of the Drosophila alcohol dehydrogenase (Adh) distal promoter is controlled by the Adh adult enhancer (AAE). Within this 150 bp, complex enhancer is a small (12 bp) positive cis-acting element that is required for high levels of distal transcription in adult flies and ADH-expressing tissue culture cells. We previously reported that the steroid receptor superfamily member FTZ-F1 binds to this site. We have identified a second steroid receptor superfamily member, DHR39, which also binds to this site. DHR39 is expressed throughout development in transcripts of several sizes. In situ hybridization to embryos has shown that DHR39 RNA is found primarily in the central nervous system, and not in embryonic tissues that express ADH. FTZ-F1 RNA, however, shows temporal-specific patterns similar to those of the distal promoter. FTZ-F1 and DHR39 have identical amino acids in the 'P-box' of the DNA binding domain, suggesting that they have identical DNA recognition characteristics. By electrophoretic mobility shift analysis we show that a DHR39 fusion protein binds specifically to two FTZ-F1 binding sites. By over expressing the full length DHR39 protein in a transient co-transfection assay we have shown that it represses distal Adh expression in a dosage- and binding site-dependent manner. Over expression of an alternative DHR39 open reading frame that lacks part of the putative ligand binding domain does not alter Adh expression. In contrast, over expression of FTZ-F1 specifically activates distal Adh expression.

Intrapelvic extraction of a total hip prosthesis. A case report.

A 69-year-old man with a total hip prosthesis, recurrent infection, and intrapelvic incarceration of the acetabular components required extraction of a long-stem total hip prosthesis through three incisions. Retroperitoneal intrapelvic extraction of the incarcerated prosthesis was necessary. This approach allowed removal of intrapelvically migrated components and the eradication of the infection. The patient had no signs of infection five years postoperatively.

Visions of rural aging: use of photographic method in gerontological research.

The use of photography is described as a method embedded within an ethnographic investigation of rural home care for older adults. Photography fostered data generation, elicited participants' stories, and illustrated patterns of rural aging. Analysis of photographic data pooled with interview and observational data facilitated an in-depth understanding of rural aging and home health care.

The vascular history and physical examination.

The value of the vascular examination cannot be over-estimated. Symptoms of vascular disease present in the foot and lower extremity may actually be manifestations of severe life-threatening disease. Symptoms, their location, and the frequency and quality of the patient's pain often provide valuable clues for the clinician's diagnosis. Central nervous system symptoms, ocular disturbances, cardiac symptoms, impotence, or constitutional disturbances may all indicate systemic arterial disease. Risk factors for this disease include smoking, hypertension, hyperlipidemia, genetic predisposition, diabetes, emotional stress, and physical inactivity. Those factors attributable to hypercoagulability and venous disease are birth control pill use, estrogen chemotherapy, obesity, prolonged immobilization, paralysis, previous thrombotic episodes, venous stasis disease, and varicose veins. An accurate bilateral assessment of blood pressure, pulses, and capillary perfusion is of critical importance. Careful inspection of the extremity for trophic changes, skin color, texture, temperature, edema, ulceration, atrophy, or paresis, will provide clues of vasculopathy. A relatively accurate assessment of circulatory status may be obtained without the use of exotic instruments. Simple tests such as the elevation and dependency tests, capillary bed return test, venous filling time test, along with blood pressure, pulse, and possibly oscillometry data are valuable in arterial evaluation. Such venous tests as inspection, percussion, Homan's sign, Trendelenburg, and Perthes' tourniquet are useful in the determination of the presence of venous disease. Fortunately, over the past few years tremendous advances have been made in the technology of the vascular laboratory. If symptoms are discovered during the vascular history and physical examination, the complete noninvasive study will provide impressive data to quantitate and specifically establish the diagnosis.

Perceived health, self-esteem, health habits, and perceived benefits and barriers to exercise in women who have and who have not experienced stage I breast cancer.

As more women survive the experience of breast cancer, it is essential to discover the impact it has on a woman's life. The purpose of this study was to determine differences in perceived health, self-esteem, health habits, and perceived benefits and barriers to exercise in women who have and who have not experienced stage I breast cancer and its treatment. A random sample of 55 women who have experienced stage I breast cancer and have not received adjuvant therapy was selected and matched to a cohort of women in the community who had not experienced cancer. No significant differences were found in perceived health, self-esteem, and health habits between women who had experienced breast cancer and the matched cohorts. There was a significant difference in both perceived benefits and barriers to exercise between groups (t = 2.4, df = 106, p = 0.18), with the matched cohorts scoring higher on perceived benefits and barriers to exercise.

Malignant fibrous histiocytoma associated with total hip replacement. A case report.

This report documents another case of malignancy associated with total hip replacement. A malignant fibrous histiocytoma of the femur was diagnosed approximately 10 years after insertion of a cemented Müller cobalt-chromium prosthesis.

Brain imaging abnormalities in mental disorders of late life.

Psychiatric inpatients with dementia (N = 61) or depression (N = 67) in late life were 2.6 times more likely to manifest magnetic resonance imaging abnormalities of the brain than were elderly controls (N = 44). Controlling for the effects of age and gender, demented patients were distinguishable from controls by an increased prevalence of cortical atrophy and infarction, while depressed patients exhibited an increased prevalence of cortical infarctions and leukoencephalopathy. Patients with dementia were distinguishable from those with major depression by an increased prevalence of cortical atrophy. These results indicate that major depression in late life, like dementia, is associated with a remarkable increase in overt pathologic changes in the brain.

The survey: what to expect.

Although your hospital will be surveyed only every two or three years, the results of that survey are vital not only to your hospital, but also to your department. And you need to become involved in the process from the presurvey questionnaire to the on-site visit. And you need to prepare for the next survey the day after the last one.