RESUMEN
Burn patients are immunocompromised yet paradoxically are able to effectively reject allogeneic skin grafts. Failure to close a massive burn wound leads to sepsis and multiple system organ failure. Immune suppression early (3 days) after burn injury is associated with glucocorticoid-mediated T cell apoptosis and anti-inflammatory cytokine responses. Using a mouse model of burn injury, we show CD8+ T cell hyperresponsiveness late (14 days) after burn injury. This is associated with a CD8+ T cell pro- and anti-inflammatory cytokine secretion profile, peripheral lymphopenia, and accumulation of a rapidly cycling, hyperresponsive memory-like CD8+CD44+ IL-7R- T cells which do not require costimulation for effective Ag response. Adoptive transfer of allospecific CD8+ T cells purified 14 days postburn results in enhanced allogeneic skin graft rejection in unburned recipient mice. Chemical blockade of glucocorticoid-induced lymphocyte apoptosis early after burn injury abolishes both the late homeostatic accumulation of CD8+ memory-like T cells and the associated enhanced proinflammatory CD8+ T cell response, but not the late enhanced CD8+ anti-inflammatory response. These data suggest a mechanism for the dynamic CD8+ T cell response following injury involving an interaction between activation, apoptosis, and cellular regeneration with broad clinical implications for allogeneic skin grafting and sepsis.
Asunto(s)
Quemaduras/inmunología , Linfocitos T CD8-positivos/inmunología , Linfocitos T CD8-positivos/patología , Proliferación Celular , Rechazo de Injerto/inmunología , Homeostasis/inmunología , Linfopenia/inmunología , Trasplante de Piel/inmunología , Traslado Adoptivo , Animales , Apoptosis/efectos de los fármacos , Apoptosis/inmunología , Quemaduras/patología , Linfocitos T CD8-positivos/trasplante , Ciclo Celular/inmunología , Citocinas/biosíntesis , Femenino , Rechazo de Injerto/patología , Inhibidores de Crecimiento/administración & dosificación , Memoria Inmunológica , Activación de Linfocitos/inmunología , Recuento de Linfocitos , Linfopenia/patología , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Mifepristona/administración & dosificación , Trasplante de Piel/patología , Subgrupos de Linfocitos T/inmunología , Subgrupos de Linfocitos T/patología , Factores de TiempoRESUMEN
CD8 engagement is believed to be a critical event in the activation of naive T cells. In this communication, we address the effects of peptide-MHC (pMHC)/TCR affinity on the necessity of CD8 engagement in T cell activation of primary naive cells. Using two peptides with different measured avidities for the same pMHC-TCR complex, we compared biochemical affinity of pMHC/TCR and the cell surface binding avidity of pMHC/TCR with and without CD8 engagement. We compared early signaling events and later functional activity of naive T cells in the same manner. Although early signaling events are altered, we find that high-affinity pMHC/TCR interactions can overcome the need for CD8 engagement for proliferation and CTL function. An integrated signal over time allows T cell activation with a high-affinity ligand in the absence of CD8 engagement.