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1.
Eksp Klin Farmakol ; 67(3): 73-5, 2004.
Artículo en Ruso | MEDLINE | ID: mdl-15341076
4.
Artículo en Ruso | MEDLINE | ID: mdl-2990125

RESUMEN

Seventeen pure lines of S. typhi bacteriophages have been obtained from mother races O and Vi; of these, three were used to study 152 S. typhi strains with a view of detecting their DNA host specificity systems. 10 S. typhi strains having the DNA host specificity system have been detected by the rough determination of the lytic spectrum and the cross titration of phage Vi IX.


Asunto(s)
Enzimas de Restricción del ADN/aislamiento & purificación , Salmonella typhi/patogenicidad , Adsorción , Bacteriólisis , Clonación Molecular/métodos , Enzimas de Restricción del ADN/farmacología , Fenotipo , Fagos de Salmonella/enzimología , Fagos de Salmonella/genética , Fagos de Salmonella/aislamiento & purificación , Salmonella typhi/enzimología , Salmonella typhi/genética , Especificidad de la Especie , Replicación Viral/efectos de los fármacos
5.
Vopr Virusol ; (1): 97-100, 1981.
Artículo en Ruso | MEDLINE | ID: mdl-7257328

RESUMEN

DNA of H-1, H-5, H-10, H-17, and H-22 phages were studied, their nucleotide composition, their buoyant densities in cesium chloride and cesium sulphate, curves of melting and spectra of circular dichroism were determined 5-oxymethylcytosine in the composition of H-I and H-22 phage DNA was shown immunologically to be glucozylated.


Asunto(s)
Bacteriófagos/análisis , ADN Viral , 5-Metilcitosina/análogos & derivados , Centrifugación por Gradiente de Densidad , Fenómenos Químicos , Química Física , Dicroismo Circular , Citosina/análogos & derivados , Citosina/análisis , Calor , Nucleótidos/análisis , Shigella , Especificidad de la Especie
6.
Vopr Virusol ; (6): 728-31, 1980.
Artículo en Ruso | MEDLINE | ID: mdl-7222633

RESUMEN

Physico-chemical properties of dysentery therapeutic-prophylactic Newcastle phages H-I, H-5, H-10, H-22 were studied. The morphology of the phage particles is described; their molecular weight and buoyant density in CsCl were determined by ultracentrifugation. The type of nucleic acids (DNA) was determined. By all the above characteristics and their chemical composition, bacteriophages of the Newcastle group approach T-even phages.


Asunto(s)
Bacteriófagos/análisis , Virus de la Enfermedad de Newcastle/análisis , Bacteriófagos/ultraestructura , Fenómenos Químicos , Química Física , Microscopía Electrónica , Virus de la Enfermedad de Newcastle/ultraestructura , Shigella , Ultracentrifugación
9.
Vopr Virusol ; (5): 557-63, 1975.
Artículo en Ruso | MEDLINE | ID: mdl-174322

RESUMEN

Some physico-chemical properties of dysentery therapeutic-prophylactic Newcastle-phages H-17 and H-18 were studied. Both phages were shown to be resistant to sodium dodecyl sulphate at room temperature. Phage H-17 at room temperature was completely inactivated and phage H-18 inactivated by 40% by 1 M sodium hydrochloride. Phage H-18 was completely inactivated by 4 M sodium hydrochloride. Morphology and sizes of the phages are described. The buoyant density of phage H-17 was found to be 1.497 g/cm3, and of phage H-18 1.504 g/cm3. DNA of both phages were identified as double-stranded on the basis of the pattern of melting curve, of interaction with formaldehyde and absorption spectrum in solutions with various ionic strengths. The melting point of DNA of phage H-17 is 83.63 degrees C, phage H-18 89.67 degrees.


Asunto(s)
Bacteriófagos/análisis , Shigella flexneri , Bacteriólisis , Bacteriófagos/efectos de los fármacos , Bacteriófagos/ultraestructura , Fraccionamiento Celular , Centrifugación por Gradiente de Densidad , Fenómenos Químicos , Química Física , Cromatografía DEAE-Celulosa , ADN Viral/análisis , Lípidos/análisis , Percloratos/farmacología , ARN Viral/análisis , Dodecil Sulfato de Sodio/farmacología , Espectrofotometría Atómica , Temperatura , Ultracentrifugación , Proteínas Virales/análisis
10.
Zh Mikrobiol Epidemiol Immunobiol ; 0(9): 52-4, 1975 Sep.
Artículo en Ruso | MEDLINE | ID: mdl-1199589

RESUMEN

A study was made of morphology of 6 clones of Newcastle bacteriophages of different origin divided into 3 types. Bacteriophage H-18 referred to the III morphological type by the Tikhonenko classification was characterized by a comparatively short process and a head in the form of an isometric polyhedron; H-1, H-5, H-10 and H-17 bacteriophages referred to type V, despite their antigenic difference were morphologically identical: they had a comparatively large head in the form of an elongated polyhedron and a process with a complicated structure ending by a besal plate with 3 indentions originating from it. Bacteriophage H-4 was referred to the IV type and was characterized by a head in the form of an elongated polyhedron and a long curved noncontracting process; in difference from the others it had no basal plate on the end of the process. The revealed morphological peculiarities of the particles of the Newcastle bacteriophages only partially correlated with their division on the basis of serolological properties and the size of the negative colonies.


Asunto(s)
Bacteriófagos/ultraestructura , Virus de la Enfermedad de Newcastle , Bacteriófagos/clasificación , Disentería Bacilar/microbiología , Aguas del Alcantarillado
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