RESUMEN
The results of experiments on rats showed a high healing potential of three collagen-based coating materials containing a combination of biologically active substances including human leukocyte interferon, alfalfa extract, and eubiotic bactisporin. The application of new materials significantly accelerated the healing of model wounds.
Asunto(s)
Colágeno/farmacología , Factores Inmunológicos/farmacología , Interferones/farmacología , Preparaciones de Plantas/farmacología , Probióticos/farmacología , Cicatrización de Heridas/efectos de los fármacos , Animales , Bacillus subtilis , Colágeno/administración & dosificación , Colágeno/toxicidad , Preparaciones de Acción Retardada , Combinación de Medicamentos , Humanos , Factores Inmunológicos/administración & dosificación , Factores Inmunológicos/toxicidad , Interferones/administración & dosificación , Interferones/toxicidad , Ratones , Preparaciones de Plantas/administración & dosificación , Preparaciones de Plantas/toxicidad , Probióticos/administración & dosificación , Probióticos/toxicidad , Conejos , Ratas , Especificidad de la EspecieRESUMEN
The effect of eracond on some parameters of immunity and allergic reactions was studied in experiments on mice and guinea pigs. The drug was found to possess an immunomodulating effect.
Asunto(s)
Adyuvantes Inmunológicos/farmacología , Medicago sativa/química , Animales , Formación de Anticuerpos/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Cobayas , Inmunidad Celular/efectos de los fármacos , Inmunización , Masculino , Ratones , Ratones Endogámicos CBA , Anafilaxis Cutánea Pasiva/efectos de los fármacos , Extractos Vegetales/farmacologíaRESUMEN
Seventeen pure lines of S. typhi bacteriophages have been obtained from mother races O and Vi; of these, three were used to study 152 S. typhi strains with a view of detecting their DNA host specificity systems. 10 S. typhi strains having the DNA host specificity system have been detected by the rough determination of the lytic spectrum and the cross titration of phage Vi IX.
Asunto(s)
Enzimas de Restricción del ADN/aislamiento & purificación , Salmonella typhi/patogenicidad , Adsorción , Bacteriólisis , Clonación Molecular/métodos , Enzimas de Restricción del ADN/farmacología , Fenotipo , Fagos de Salmonella/enzimología , Fagos de Salmonella/genética , Fagos de Salmonella/aislamiento & purificación , Salmonella typhi/enzimología , Salmonella typhi/genética , Especificidad de la Especie , Replicación Viral/efectos de los fármacosRESUMEN
DNA of H-1, H-5, H-10, H-17, and H-22 phages were studied, their nucleotide composition, their buoyant densities in cesium chloride and cesium sulphate, curves of melting and spectra of circular dichroism were determined 5-oxymethylcytosine in the composition of H-I and H-22 phage DNA was shown immunologically to be glucozylated.
Asunto(s)
Bacteriófagos/análisis , ADN Viral , 5-Metilcitosina/análogos & derivados , Centrifugación por Gradiente de Densidad , Fenómenos Químicos , Química Física , Dicroismo Circular , Citosina/análogos & derivados , Citosina/análisis , Calor , Nucleótidos/análisis , Shigella , Especificidad de la EspecieRESUMEN
Physico-chemical properties of dysentery therapeutic-prophylactic Newcastle phages H-I, H-5, H-10, H-22 were studied. The morphology of the phage particles is described; their molecular weight and buoyant density in CsCl were determined by ultracentrifugation. The type of nucleic acids (DNA) was determined. By all the above characteristics and their chemical composition, bacteriophages of the Newcastle group approach T-even phages.
Asunto(s)
Bacteriófagos/análisis , Virus de la Enfermedad de Newcastle/análisis , Bacteriófagos/ultraestructura , Fenómenos Químicos , Química Física , Microscopía Electrónica , Virus de la Enfermedad de Newcastle/ultraestructura , Shigella , UltracentrifugaciónRESUMEN
Some physico-chemical properties of dysentery therapeutic-prophylactic Newcastle-phages H-17 and H-18 were studied. Both phages were shown to be resistant to sodium dodecyl sulphate at room temperature. Phage H-17 at room temperature was completely inactivated and phage H-18 inactivated by 40% by 1 M sodium hydrochloride. Phage H-18 was completely inactivated by 4 M sodium hydrochloride. Morphology and sizes of the phages are described. The buoyant density of phage H-17 was found to be 1.497 g/cm3, and of phage H-18 1.504 g/cm3. DNA of both phages were identified as double-stranded on the basis of the pattern of melting curve, of interaction with formaldehyde and absorption spectrum in solutions with various ionic strengths. The melting point of DNA of phage H-17 is 83.63 degrees C, phage H-18 89.67 degrees.
Asunto(s)
Bacteriófagos/análisis , Shigella flexneri , Bacteriólisis , Bacteriófagos/efectos de los fármacos , Bacteriófagos/ultraestructura , Fraccionamiento Celular , Centrifugación por Gradiente de Densidad , Fenómenos Químicos , Química Física , Cromatografía DEAE-Celulosa , ADN Viral/análisis , Lípidos/análisis , Percloratos/farmacología , ARN Viral/análisis , Dodecil Sulfato de Sodio/farmacología , Espectrofotometría Atómica , Temperatura , Ultracentrifugación , Proteínas Virales/análisisRESUMEN
A study was made of morphology of 6 clones of Newcastle bacteriophages of different origin divided into 3 types. Bacteriophage H-18 referred to the III morphological type by the Tikhonenko classification was characterized by a comparatively short process and a head in the form of an isometric polyhedron; H-1, H-5, H-10 and H-17 bacteriophages referred to type V, despite their antigenic difference were morphologically identical: they had a comparatively large head in the form of an elongated polyhedron and a process with a complicated structure ending by a besal plate with 3 indentions originating from it. Bacteriophage H-4 was referred to the IV type and was characterized by a head in the form of an elongated polyhedron and a long curved noncontracting process; in difference from the others it had no basal plate on the end of the process. The revealed morphological peculiarities of the particles of the Newcastle bacteriophages only partially correlated with their division on the basis of serolological properties and the size of the negative colonies.