RESUMEN
Percutaneous transluminal coronary angioplasty (PTCA) was performed on 725 occasions at the National Hospital of Norway in 1981-1990. Acute surgical intervention was necessary within 24 hours after PTCA in 15 cases, on indications that included coronary artery dissection (8 cases) and acute thrombotic occlusion (5). Electrocardiographic signs of ischemia were present in 11 patients with anginal pain, while two had severe angina but normal electrocardiogram. Two patients had a non-ischemic indication for surgery. Despite surgery, ten of the 15 patients had acute myocardial infarction. One of the ten died. At follow-up nine of 12 patients were free from angina and three had recurrent symptoms.
Asunto(s)
Angioplastia Coronaria con Balón , Puente de Arteria Coronaria , Enfermedad Coronaria/terapia , Enfermedad Coronaria/epidemiología , Enfermedad Coronaria/cirugía , Electrocardiografía , Urgencias Médicas , Femenino , Humanos , Incidencia , Masculino , Persona de Mediana Edad , Infarto del Miocardio/epidemiología , Infarto del Miocardio/cirugía , Isquemia Miocárdica/epidemiología , Isquemia Miocárdica/cirugía , Factores de Tiempo , Insuficiencia del TratamientoRESUMEN
Intralipid was continuously infused into the thoracic duct for 12 days in rats. All remained clinically fit and had satisfactory weight gain only for the first week. Autopsy showed significantly increased lung weight in all rats and in some there were lymphatic fistulas and dilated thoracic duct. Microscopy revealed fat droplets, vascular changes and thickening of alveolar septa in the lungs. Epithelioid cell clusters were found in liver and spleen in some of the rats and Kupffer cell increase in most. Thus commercially available fat emulsion for parenteral use, when delivered by the natural route for natural chylomicrons, caused undesirable derangements in rats.
Asunto(s)
Emulsiones Grasas Intravenosas/administración & dosificación , Conducto Torácico , Animales , Catéteres de Permanencia , Hígado/efectos de los fármacos , Pulmón/efectos de los fármacos , Masculino , Tamaño de los Órganos/efectos de los fármacos , Ratas , Ratas Endogámicas , Factores de TiempoRESUMEN
A rapid assay of O6-MeG-DNA methyltransferase activity is described. Following incubation of cell extracts with O6-[3H]MeG-containing DNA, remaining radioactive DNA was hydrolyzed in trichloroacetic acid and separated from methylated radioactive protein by filtration or centrifugation. Transfer of radioactive methyl from DNA to protein was proportional to the amount of protein added, and was not linear with time. More than 90% of the radioactivity precipitated after acid hydrolyses was in S-methyl cysteine residues. The method was used to measure O6-MeG-DNA methyltransferase activity in extracts of 24 neoplastic tissues from human organs. Although five tumor tissues had 28-84% lower activity of O6-MeG-DNA methyltransferase than the corresponding normal tissue from the same patient, higher or similar levels of activity were found more frequently. Thus, a lack of O6-MeG-DNA methyltransferase activity in human tumours appears not to be a frequent event. The DNA repair enzyme uracil-DNA glycosylase was also measured in the same extracts. Most frequently the level of uracil-DNA glycosylase activity was essentially similar in tumors and normal tissues but significantly higher or lower levels were also observed.
