1.
Ann Parasitol Hum Comp
; 61(2): 147-54, 1986.
Artículo
en Francés
| MEDLINE
| ID: mdl-3524401
RESUMEN
Several species of leishmania and three methods of cultivation: monophasic, biphasic and co-cultivation were used in a compared study bearing on the intensive production of leishmania. In addition by applying, a new in vivo model, comprising an injection of sarcomatous cells and promastigotes into BALB/c mice and also an extraction on a discontinuous gradient (Radioselectan 60%), it was possible to obtain highly purified isolates of amastigote forms. The use of two antigens: promastigotes and amastigotes, is to be recommended for the serological diagnosis, by indirect immunofluorescence, of kala-azar. The new in vivo model merits further consideration for research concerning new molecules active against leishmania.
Asunto(s)
Antimonio/uso terapéutico , Antiprotozoarios/uso terapéutico , Leishmania/crecimiento & desarrollo , Meglumina , Compuestos Organometálicos , Animales , Línea Celular , Medios de Cultivo , Técnica del Anticuerpo Fluorescente , Leishmaniasis/tratamiento farmacológico , Antimoniato de Meglumina , Ratones , Ratones Endogámicos BALB C , Pruebas Serológicas , Factores de Tiempo
2.
Planta Med
; 40(4): 356-60, 1980 Dec.
Artículo
en Francés
| MEDLINE
| ID: mdl-7220649
3.
Ann Soc Belg Med Trop
; 58(3): 241-9, 1978.
Artículo
en Francés
| MEDLINE
| ID: mdl-747432