Increased virulence due to multiple infection in Daphnia leads to limited growth in 1 of 2 co-infecting microsporidian parasites.

Recent outbreaks of various infectious diseases have highlighted the ever-present need to understand the drivers of the outbreak and spread of disease. Although much of the research investigating diseases focuses on single infections, natural systems are dominated by multiple infections. These infections may occur simultaneously, but are often acquired sequentially, which may alter the outcome of infection. Using waterfleas (Daphnia magna) as a model organism, we examined the outcome of sequential and simultaneous multiple infections with 2 microsporidian parasites (Ordospora colligata and Hamiltosporidium tvaerminnensis) in a fully factorial design with 9 treatments and 30 replicates. We found no differences between simultaneous and sequential infections. However, H. tvaerminnensis fitness was impeded by multiple infection due to increased host mortality, which gave H. tvaerminnensis less time to grow. Host fecundity was also reduced across all treatments, but animals infected with O. colligata at a younger age produced the fewest offspring. As H. tvaerminnensis is both horizontally and vertically transmitted, this reduction in offspring may have further reduced H. tvaerminnensis fitness in co-infected treatments. Our findings suggest that in natural populations where both species co-occur, H. tvaerminnensis may evolve to higher levels of virulence following frequent co-infection by O. colligata.

Infection with behaviour-manipulating parasites enhances bioturbation by key aquatic detritivores.

The ecological ubiquity of parasites and their potential impacts on host behaviour have led to the suggestion that parasites can act as ecosystem engineers, structuring their environment and physical habitats. Potential modification of the relationship between parasites and their hosts by climate change has important implications for how hosts interact with both their biotic and abiotic environment. Here, we show that warming and parasitic infection independently increase rates of bioturbation by a key detritivore in aquatic ecosystems (Gammarus). These findings have important implications for ecosystem structure and functioning in a warming world, as alterations to rates of bioturbation could significantly modify oxygenation penetration and nutrient cycling in benthic sediments of rivers and lakes. Our results demonstrate a need for future ecosystem management strategies to account for parasitic infection when predicting the impacts of a warming climate.