RESUMEN
The egg positive rate of Enterobius vermicularis and Clonorchis sinensis of school children in the rural area was studied in Hamyang-gun, Gyeongsangnam-do in Korea. Cellotape anal swab and formalin ether concentration methods were performed one time to 720 primary school children. The total egg positive rate of E. vermicularis was 12.6% in two schools (Baekjeon and Wiseong). In the Baekjeon and Wiseong primary school, the egg positive rate of E. vermicularis was 4.6% and 13.4%, respectively. Pinworm egg positive rate was 17.6% in the lower grades (1st, 2nd and 3rd), and 7.7% in higher grades (4th, 5th and 6th). The total egg positive rate of male and female was 12.6% and 12.7%, respectively. The egg positive rate of C. sinensis of Baekjeon and Wiseong primary school was 1.5% and 0.46%, respectively. The total egg positive rate of C. sinesis was 0.56%. This survey showed that continuous education and chemotherapy is necessary to treat and prevent reinfection of E. vermicularis. In the case of C. sinensis, health education for school children is recommended to prevent potential infection of adolescents.
Asunto(s)
Clonorquiasis/epidemiología , Enterobiasis/epidemiología , Parasitosis Intestinales/epidemiología , Adolescente , Niño , Clonorquiasis/prevención & control , Enterobiasis/prevención & control , Femenino , Educación en Salud , Humanos , Parasitosis Intestinales/prevención & control , Corea (Geográfico)/epidemiología , Masculino , Recuento de Huevos de Parásitos/estadística & datos numéricos , Prevalencia , Población Rural , Instituciones Académicas/estadística & datos numéricosAsunto(s)
Acanthamoeba/enzimología , ADN Complementario/genética , Proteínas Protozoarias , Serina Endopeptidasas/genética , Serina Endopeptidasas/metabolismo , Subtilisinas/genética , Subtilisinas/metabolismo , Acanthamoeba/genética , Amebiasis/parasitología , Secuencia de Aminoácidos , Animales , Northern Blotting , Southern Blotting , Western Blotting , Dominio Catalítico , Encefalitis/parasitología , Genes Protozoarios , Granuloma/parasitología , Humanos , Datos de Secuencia Molecular , Serina Endopeptidasas/química , Subtilisinas/químicaRESUMEN
The taxonomic validity of morphological group III Acanthamoeba spp. is uncertain. In the present study, six type strains of group III Acanthamoeba spp., A. culbertsoni, A. healyi, A. pustulosa, A. palestinensis, A. royreba and A. lenticulata were subjected for the evaluation of their taxonomic validity by comparison of the isoenzyme patterns by isoelectic focusing on polyacrylamide gels, mitochondrial DNA (Mt DNA) restriction fragment length polymorphism (RFLP), and small subunit ribosomal DNA (ssu rDNA) PCR-RFLP patterns. The Mt DNA RFLP patterns were heterogeneous between the species. The type strains of A. palestinensis and A. pustulosa showed almost identical patterns of isoenzymes and rDNA PCR RFLP with an estimated sequence divergence of 2.6%. The other species showed heterogeneous patterns of isoenzymes and rDNA PCR-RFLP. It is likely that A. pustulosa is closely related with A. palestinensis and that the former may be regarded as a junior synonym of the latter.
