[Study of hepatitis type B during hemodialysis]. / Izuchenie gepatita tipa B pri gemodialize.

Blood sera of 29 patients of the hemodialysis ward were regularly examined 6 months and later after hospitalization for the presence of HBsAg and anti-HBs (anti-HBs were found in 4 patients, HBsAg in one). The results of the study in one patient who developed hepatitis B infection of the dynamics of changes in specific and nonspecific values in the incubation period and in the period of manifest clinical signs of the disease are presented.

[Liberation of DNA from particles of CD phage of DNA-protein complex: Properties of DNA in the complex and the effect of formaldehyde on the complex structure]. / Osvobozhdenie DNK iz chastits faga CD v vide kompleksa s belkom: svoístva DNK v sostave kompleksa i vliianie formal'degida na strukturu kompleksa

Properties of DNA in a complex with protein, which was liberated after destruction of CD phage by heat treatment in solutions with low ionic strength, were studied. DNA in the complex did not differ from free DNA under the same conditions as shown by spectra of circular dichroism and by the type of melting during the thermic denaturation. As demonstrated by viscosimetry and gradient centrifugation in cesium sulfate, 1.5% formaldehyde inhibited the dissociation of the complex studied in a medium containing 0.15 M NaCl. In the medium with 1.5% of HCHO T degrees ml of DNA in the complex was distinctly higher than T degrees ml of free DNA under the same conditions. Electron microscopy showed that the complex studied comprised DNA ;molecules, associated with protein membrane in one or several internal sites.

[Release of DNA from Sd phage in a protein complex]. / Osvobozhdenie DNK iz chastits faga Sd v vide kompleksa s belkom

The properties of DNA released from Cd phage upon destruction of virions by heating in solutions of low strength were studied. The viscosity of DNA released from the phage after heating in 0.01 X SSC (SSC-0.15 M NaCl divided by 0.015 M sodium citrate, pH 7.0) was found to be no more than 40% of that of DNA released from the phage destroyed by heating in 1 X SSC. Addition of salts up to 1 X SSC to the phage destroyed by heatings in 0.01 X SSC resulted in a marked increase of the viscosity of the latter. According to the results of centrifugation in cesium sulphate and sucrose density gradients, upon phage destruction by heating in 0.01 X SSC DNA war released from virions in the form of a complex with protein. Addition of NaCl up to 1 X SSC to the phage preparations destroyed in the low ionic strength solution led to destruction of this complex. The complex under study was not formed in dialysis against 0.01 X SSC of phage preparations destroyed in 1 X SSC.