RESUMEN
Two new HLA-C alleles have been identified by reverse line blot assay and direct sequencing of polymerase chain reaction (PCR) products. The first polymorphism encodes an amino acid change from lysine in Cw*12022 to asparagine in Cw*1208 at residue 66. The second polymorphism encodes two amino acid changes from isoleucine in Cw*03031 to threonine in Cw*0313 at residue 93 and isoleucine to leucine at residue 94. The functional significance of these polymorphisms on peptide-binding and/or T-cell recognition is unknown.
Asunto(s)
Alelos , Antígenos HLA-C/genética , Genes MHC Clase I , Prueba de Histocompatibilidad , Humanos , Modelos Genéticos , Datos de Secuencia Molecular , Síndromes Mielodisplásicos/genética , Síndromes Mielodisplásicos/patología , Análisis de Secuencia de ADN , Análisis de Secuencia de ProteínaRESUMEN
A novel polymorphism was identified in a B*07 allele. B*0722 possesses a unique nucleotide substitution at nucleotide 481. This polymorphism encodes an amino acid difference from aspartic acid in B*07021 to asparagine in B*0722. The functional significance of this polymorphism on peptide binding and/or T-cell recognition is unknown.
Asunto(s)
Antígenos HLA-B/genética , Polimorfismo de Nucleótido Simple , Antígeno HLA-B7 , Humanos , Mieloma Múltiple/genética , Mieloma Múltiple/inmunologíaRESUMEN
We show here the use of reference strand mediated conformation analysis (RSCA) to unambiguously resolve the HLA-DRB1 typing of two individuals which were selected as potential unrelated donors for bone marrow transplantation (BMT). In the first case, both sequence-specific primer (SSP) amplification and sequence-specific oligonucleotide probing (SSO), routinely used in different tissue typing laboratories gave, for the two unrelated donors, the same ambiguous typing of HLA-DRB1*04011+*0403 or DRB1*0407+*0413. In this case sequence-based typing (SBT) was not the method of choice to resolve the situation, due to the sequence ambiguities of these two given combinations. RSCA of both samples, using homozygous typing cells (HTCs) for DRB1*04011, *0403 and *0407 as internal controls, gave the unambiguous result that both donors were HLA-DRB1*04011+*0403. In the second case, a donor was typed as DRB1*1102+1103 by SSP, while SSO excluded the DRB1*1102 allele. The patient was unambiguously typed as DRB1*1101+1103 by both techniques. RSCA, using DNA from reference cell lines as internal controls, gave the unambiguous typing that the donor was DRB1*1103 homozygous.
Asunto(s)
Trasplante de Médula Ósea , Antígenos HLA-DR/genética , Prueba de Histocompatibilidad/métodos , Polimorfismo Conformacional Retorcido-Simple , Alelos , Cartilla de ADN , Cadenas HLA-DRB1 , Humanos , Sondas de OligonucleótidosRESUMEN
Human HLA genes exhibit extreme polymorphism, the extent of which is emphasised by the identification of an ever increasing number of new alleles by DNA-based typing strategies. Here we describe a novel allele, belonging to the HLA-B*15 group, which was identified in an African patient awaiting a bone marrow transplant This individual was shown to exhibit two HLA-B alleles, B*5301 and a new allele which has been named B*1555. B*1555 differs from B*1531 in exon 3 by a single nucleotide substitution. This substitution results in a change in the amino acid residue at position 97, which is located within the beta-pleated sheet region of the HLA molecule.
Asunto(s)
Población Negra/genética , Trasplante de Médula Ósea/inmunología , Antígenos HLA-B/genética , África/etnología , Alelos , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Secuencia de Bases , Exones , Humanos , Datos de Secuencia Molecular , Polimorfismo de Nucleótido Simple , Análisis de Secuencia de ADN , Reino UnidoRESUMEN
Incentive spirometry offered no statistically significant advantages to pulmonary function when compared to unstructured or structured deep breathing and coughing exercise programs, for patients at low risk of developing pulmonary complications. The additional cost of incentive spirometer equipment does not seem warranted in these patients. Furthermore, patients with or without an incentive spirometer were willing to comply with a structured breathing exercise program with the same frequency of practice sessions. Patients in this diagnostic category did not require a technical device to reward and motivate them for performing maximal inspiratory manoeuvres.
Asunto(s)
Neumonía/prevención & control , Complicaciones Posoperatorias/prevención & control , Espirometría/normas , Adulto , Estudios de Evaluación como Asunto , Humanos , Persona de Mediana Edad , Motivación , Cooperación del Paciente , Neumonía/epidemiología , Neumonía/psicología , Complicaciones Posoperatorias/epidemiología , Complicaciones Posoperatorias/psicologíaAsunto(s)
Actitud Frente a la Muerte , Muerte , Eutanasia , Tanatología , Anciano , Humanos , Masculino , Estados UnidosRESUMEN
This report deals with the preliminary findings of a research project on simultaneous communication and multisensory input in the treatment of six autistic and communication disordered children. The children, aged 5 to 12, were taught manually signed English and speech using a multisensory-intrusion approach. The hypothesis was that such a technique would serve to alleviate the children's difficulties in information processing, organization of experience, and affect. The dependent measures were behavioral ratings derived from both structured (teaching) and unstructured (free play) sessions. The results indicate that the children manifested a consistent acquisition of sign lanuage, which in some cases transferred into verbal communication skills. Moreover, statistical analyses of some of the observed socioaffective behaviors (i.e., nonsolitary play, interaction with peers and adults, exploration, and detachment) revealed tendencies supportive of the hypothesis. The variability of the data preclude any categorical statement in relation to the hypothesis. However, the preliminary results strongly support the continuation of the study.
