RESUMEN
Bioluminescence from cells is so dim that bioluminescence microscopy is performed using an ultra low-light imaging camera. Although the image sensor of such cameras has been greatly improved over time, such improvements have not been made commercially available for microscopes until now. Here, we customized the optical system of a microscope for bioluminescence imaging. As a result, bioluminescence images of cells could be captured with a conventional objective lens and colour imaging camera. As bioluminescence microscopy requires no excitation light, it lacks the photo-toxicity associated with fluorescence imaging and permits the long-term, nonlethal observation of living cells. Thus, bioluminescence microscopy would be a powerful tool in cellular biology that complements fluorescence microscopy.
Asunto(s)
Microscopía Fluorescente/instrumentación , Microscopía Fluorescente/métodos , Línea Celular Tumoral , Luciferina de Luciérnaga , Colorantes Fluorescentes , Humanos , Luciferasas de Luciérnaga , Mediciones LuminiscentesRESUMEN
The Coleoptera order is the richest group among Metazoa, but its phylogenetics remains incompletely understood. Among Coleoptera, bioluminescence is found within the Elateroidea, but the evolution of this character remains a mystery. Mitochondrial DNA has been used extensively to reconstruct phylogenetic relationships, however, the evolution of a single gene does not always correspond to the species evolutionary history and the molecular marker choice is a key step in this type of analysis. To create a solid basis to better understand the evolutionary history of Coleoptera and its bioluminescence, we sequenced and comparatively analyzed the mitochondrial genome of the Brazilian luminescent click beetle Pyrophorus divergens (Coleoptera: Elateridae).
Asunto(s)
Escarabajos/genética , ADN Mitocondrial/genética , Evolución Molecular , Genoma de los Insectos , Animales , Secuencia de Bases , Codón , Luminiscencia , Conformación de Ácido Nucleico , Filogenia , ARN de Transferencia/química , ARN de Transferencia/genéticaRESUMEN
Thrombasthenia is a congenital platelet disorder characterized by the normal platelet count, prolonged bleedingtime, absence of platelet aggregation and defective clot retraction. Clinically, purpura, epistaxis, gingival bleeding and excessive bleeding after minor injuries or operations are the main manifestations. We experienced a case of a 15-year-old boy suffering from thrombasthenia required extraction of 6/6. The problems of nature of the disease and its familial character are described and the problems of management are outlined.
