In vivo investigation of the inflammatory response against allylamine plasma polymer coated titanium implants in a rat model.

Titanium (Ti) is an established biomaterial for bone replacement. However, facilitation of osteoblast attachment by surface modification with chemical groups could improve the implant performance. Therefore, this study aimed to evaluate the effect of a plasma polymerized allylamine (PPAAm) layer on the local inflammation in a rat model. Three series (RM76AB, RM78AB, RM77AB) of PPAAm-treated Ti plates were prepared using different plasma conditions. Twelve male LEW.1A rats received one plate of each series and one uncoated control plate implanted into the back musculature. After 7, 14 and 56 days, four rats were euthanized to remove the implants with surrounding tissue. Total monocytes/macrophages, tissue macrophages, T-cells and MHC-class-II-positive cells were morphometrically counted. On day 14, the macrophage/monocyte number was significantly higher for the controls than for the PPAAm samples. On day 56, the RM76AB and RM78AB samples had significantly lower numbers than RM77AB and the controls. The same was found for the tissue macrophages. No change over time and no differences between the implants were found for the T-cells. For the number of MHC-class-II-positive cells, a significant decrease was found only for the RM78AB implants between day 14 and day 56. Physico-chemical analysis of the PPAAm implants revealed that the RM77AB implants had the lowest water absorption, the highest nitrogen loss and the lowest oxygen uptake after sonication. These results demonstrate that the PPAAm samples and the controls were comparable regarding local inflammation, and that different plasma conditions lead to variations in the material properties which influence the tissue reaction.

Response of primary fibroblasts and osteoblasts to plasma treated polyetheretherketone (PEEK) surfaces.

Polyetheretherketone (PEEK) is a synthetic polymer with suitable biomechanical and stable chemical properties, which make it attractive for use as an endoprothetic material and for ligamentous replacement. However, chemical surface inertness does not account for a good interfacial biocompatibility, and PEEK requires a surface modification prior to its application in vivo. In the course of this experimental study we analyzed the influence of plasma treatment of PEEK surfaces on the cell proliferation and differentiation of primary fibroblasts and osteoblasts. Further we examined the possibility of inducing microstructured cell growth on a surface with plasma-induced chemical micropatterning. We were able to demonstrate that the surface treatment of PEEK with a low-temperature plasma has significant effects on the proliferation of fibroblasts. Depending on the surface treatment, the proliferation rate can either be stimulated or suppressed. The behavior of the osteoblasts was examined by evaluating differentiation parameters. By detection of alkaline phosphatase, collagen I, and mineralized extracellular matrix as parameters for osteoblastic differentiation, the examined materials showed results comparable to commercially available polymer cell culture materials such as tissue culture polystyrene (TCPS). Further microstructured cell growth was produced successfully on micropatterned PEEK foils, which could be a future tool for bioartificial systems applying the methods of tissue engineering. These results show that chemically inert materials such as PEEK may be modified specifically through the methods of plasma technology in order to improve biocompatibility.

Behaviour of biofilm systems under varying hydrodynamic conditions.

Heterotrophic biofilms were cultivated in long-term experiments in biofilm tube reactors. During the biofilm cultivation the substrate loading of glucose was kept constant while the hydrodynamic conditions were changed stepwise. To describe the behaviour of the biofilm structure under these varying flow conditions the mass transfer and transport at the bulk/biofilm interface and inside the biofilm was investigated with oxygen microelectrodes. Furthermore, the biofilm density was used to describe the biofilm compactness before and after the change of the hydrodynamic condition. The obtained results show that the biofilm density and also the substrate flux decreased with decreasing flow velocity in the bulk phase. Additionally the slope of the oxygen concentration profiles decreased and the thickness of the concentration boundary layer increased. On the other hand, increasing the flow velocity in the bulk phase led both to a higher biofilm density and a higher maximum substrate flux. The biofilm surface became more homogenous and the thickness of the concentration boundary layer decreased. The time for adaptation of the biofilm structure after changing the hydrodynamic conditions ranged between 1 and 3 weeks.

Chemical micropatterning of polymeric cell culture substrates using low-pressure hydrogen gas discharge plasmas.

Micropatterned cell cultures will allow a new quality of bioartificial systems. Here, an approach to chemical micropatterning of polymer substrates is presented, which is completely based on low pressure gas discharge processes. Well expressed micropatterned cell cultures on polystyrene and poly (ether ether ketone) were obtained with many different cell types. No impairment of typical cell behavior was observed.