RESUMEN
Ethylnitrosourea-induced central and peripheral nerve tumors in Sprague-Dawley rats were tested for GFAP (Glial Fibrillary Acidic Protein), S-100 protein, NSE (Neuron Specific Enolase) and Anti-Leu 7 (HNK-1) immunoreactivity utilizing the ABC method (avidin-biotin-complex) for GFAP, S-100 protein and NSE, and the PAP method (peroxidase-antiperoxidase) for Anti-Leu 7. Peripheral nerve neurinomas were consistently positive for S-100 protein and consistently negative for GFAP and Anti-Leu 7. Neurinomas would occasionally exhibit positive staining for NSE (2 of 55 tumors). The staining intensity for S-100 protein varied from strongly positive in differentiated neurinomas to weakly positive in anaplastic tumors. Neoplastic and reactive astrocytes exhibited positive staining for both S-100 protein and GFAP. Variation in the GFAP staining intensity of glial tumors correlated with the degree of differentiation as anaplastic tumors did not stain with the same intensity as their more differentiated counterparts. Oligodendrogliomas exhibited occasional immunoreactivity to S-100 protein (3 of 36 tumors). NSE reactivity in oligodendrogliomas was rarely observed (1 tumor in 36) and immunoreactivity against GFAP or Anti-Leu 7 was consistently absent. Anti-Leu 7 and NSE proved to be of little value in the classification of ENU-induced neural tumors.
Asunto(s)
Etilnitrosourea/toxicidad , Neoplasias del Sistema Nervioso/inducido químicamente , Neoplasias del Sistema Nervioso Periférico/inducido químicamente , Animales , Antígenos de Diferenciación/metabolismo , Astrocitoma/inducido químicamente , Astrocitoma/metabolismo , Astrocitoma/patología , Antígenos CD57 , Sistema Nervioso Central/efectos de los fármacos , Sistema Nervioso Central/metabolismo , Sistema Nervioso Central/patología , Ependimoma/inducido químicamente , Ependimoma/metabolismo , Ependimoma/patología , Proteína Ácida Fibrilar de la Glía/metabolismo , Glioma/inducido químicamente , Glioma/metabolismo , Glioma/patología , Inmunohistoquímica , Meningioma/inducido químicamente , Meningioma/metabolismo , Meningioma/patología , Neoplasias del Sistema Nervioso/metabolismo , Neoplasias del Sistema Nervioso/patología , Neurilemoma/inducido químicamente , Neurilemoma/metabolismo , Neurilemoma/patología , Oligodendroglioma/inducido químicamente , Oligodendroglioma/metabolismo , Oligodendroglioma/patología , Neoplasias del Sistema Nervioso Periférico/metabolismo , Neoplasias del Sistema Nervioso Periférico/patología , Fosfopiruvato Hidratasa/metabolismo , Ratas , Ratas Endogámicas , Proteínas S100/metabolismoRESUMEN
Changes in cytoskeletal organization in T9 anaplastic glioma cells have been examined during morphological changes induced by nerve growth factor (NGF) and by glia maturation factor (GMF). Indirect immunofluorescent labeling of cytoskeletal proteins has revealed that while neither GMF nor NGF induces expression of glial fibrillary acidic protein in this cell line, changes in cytoskeletal organization induced by these factors show some features similar to those observable during maturation of normal glial cells. Changing cell shapes induced by these factors are clearly outlined by the prominent distribution of microfilaments along cellular margins. Microtubules and intermediate filaments gradually extend during morphological changes and fill the characteristic cytoplasmic processes induced by NGF and GMF.
