Alterações da matriz extracelular esplênica em cães naturalmente infectados com Leishmania (Leishmania) infantum chagasi / Changes in the splenic extracellular matrix in dogs naturally infected with Leishmania (Leishmania) infantum chagasi

The aim of this study was to study the changes in the splenic extracellular matrix of dogs naturally infected with Leishmania (Leishmania) infantum chagasi and its correlation to clinical, histopathological, and parasitological aspects. Eighteen dogs were used, separated into three groups: six non-infected animals (control group) and twelve infected animals. The dogs had undefined breed and age, from the township region of São Luís-MA. Paraffined slices of the spleen were stained with Hematoxilin and Eosin (H&E); Gomoris ammoniacal Silver, to mark reticular fibers; and the Immunohistochemistry technique of streptavidin peroxidase to detect amastigote forms of Leishmania. The morphometrical analyses were done using the KS300 program and the images analysis system Kontron Elektronic/Carl Zeiss, Germany. The results showed that there is a significant increase in the deposition of collagen fibers in the spleen, compared to control animals, showing significant differences for symptomatic and asymptomatic animals. Positive correlations were found between the presence of the parasite in the tissue and collagen deposition. Symptomatic animals showed higher collagen deposition in the spleen, which can be associated to the high parasitism found in the tissue. The results showed that there is an intense fibrogenesis in the spleen in the canine visceral leishmaniasis, thus being associated to the parasitism of the tissue and thedegenerative processes of the disease.

Objetivou-se estudar as alterações da matriz extracelular no baço de cães naturalmente infectados com Leishmania (Leishmania) infantum chagasi correlacionando-as com aspectos clínicos, histopatológicos e parasitológicos. Foram utilizados 18 cães, divididos em três grupos: seis animais não infectados (grupo controle) e doze animais infectados. Todos sem raça e idade definidas, provenientes da região do Município de São Luis-MA.  Cortes parafinados do baço foram corados pela Hematoxilina e Eosina (H&E); Prata amoniacal de Gomori, para marcação das fibras reticulares e pela técnica Imuno-histoquímica da estreptoavidina-peroxidase para detecção de formas amastigotas de Leishmania. As análises morfométricas foram feitas utilizando-se o programa KS300 e o sistema de análise de imagens Kontron Elektronic/Carl Zeiss, Germany. Os resultados mostram que há um aumento significativo da deposição de fibras colágenas no baço quando comparadas aos animais controles, revelando diferenças significativas entre os animais sintomáticos e assintomáticos. Encontraram-se correlações positivas entre a presença do parasitismo tecidual e a deposição de colágeno. Os animais sintomáticos apresentaram uma maior deposição de colágeno no baço, que pode estar associada ao maior parasitismo tecidual encontrado. Os resultados demonstram que na leishmaniose visceral canina há uma fibrogênese intensa no baço, sendo esta associada ao parasitismo tecidual e a processos degenerativos decorrentes da doença.

Alterações da matriz extracelular esplênica em cães naturalmente infectados com Leishmania (Leishmania) infantum chagasi / Changes in the splenic extracellular matrix in dogs naturally infected with Leishmania (Leishmania) infantum chagasi

The aim of this study was to study the changes in the splenic extracellular matrix of dogs naturally infected with Leishmania (Leishmania) infantum chagasi and its correlation to clinical, histopathological, and parasitological aspects. Eighteen dogs were used, separated into three groups: six non-infected animals (control group) and twelve infected animals. The dogs had undefined breed and age, from the township region of São Luís-MA. Paraffined slices of the spleen were stained with Hematoxilin and Eosin (H&E); Gomoris ammoniacal Silver, to mark reticular fibers; and the Immunohistochemistry technique of streptavidin peroxidase to detect amastigote forms of Leishmania. The morphometrical analyses were done using the KS300 program and the images analysis system Kontron Elektronic/Carl Zeiss, Germany. The results showed that there is a significant increase in the deposition of collagen fibers in the spleen, compared to control animals, showing significant differences for symptomatic and asymptomatic animals. Positive correlations were found between the presence of the parasite in the tissue and collagen deposition. Symptomatic animals showed higher collagen deposition in the spleen, which can be associated to the high parasitism found in the tissue. The results showed that there is an intense fibrogenesis in the spleen in the canine visceral leishmaniasis, thus being associated to the parasitism of the tissue and thedegenerative processes of the disease.(AU)

Objetivou-se estudar as alterações da matriz extracelular no baço de cães naturalmente infectados com Leishmania (Leishmania) infantum chagasi correlacionando-as com aspectos clínicos, histopatológicos e parasitológicos. Foram utilizados 18 cães, divididos em três grupos: seis animais não infectados (grupo controle) e doze animais infectados. Todos sem raça e idade definidas, provenientes da região do Município de São Luis-MA.  Cortes parafinados do baço foram corados pela Hematoxilina e Eosina (H&E); Prata amoniacal de Gomori, para marcação das fibras reticulares e pela técnica Imuno-histoquímica da estreptoavidina-peroxidase para detecção de formas amastigotas de Leishmania. As análises morfométricas foram feitas utilizando-se o programa KS300 e o sistema de análise de imagens Kontron Elektronic/Carl Zeiss, Germany. Os resultados mostram que há um aumento significativo da deposição de fibras colágenas no baço quando comparadas aos animais controles, revelando diferenças significativas entre os animais sintomáticos e assintomáticos. Encontraram-se correlações positivas entre a presença do parasitismo tecidual e a deposição de colágeno. Os animais sintomáticos apresentaram uma maior deposição de colágeno no baço, que pode estar associada ao maior parasitismo tecidual encontrado. Os resultados demonstram que na leishmaniose visceral canina há uma fibrogênese intensa no baço, sendo esta associada ao parasitismo tecidual e a processos degenerativos decorrentes da doença. (AU)

ALTERAÇÕES DA MATRIZ EXTRACELULAR ESPLÊNICA EM CÃES NATURALMENTE INFECTADOS COM Leishmania (Leishmania) infantum chagasi

The aim of this study was to study the changes in the splenic extracellular matrix of dogs naturally infected with Leishmania (Leishmania) infantum chagasi and its correlation to clinical, histopathological, and parasitological aspects. Eighteen dogs were used, separated into three groups: six non-infected animals (control group) and twelve infected animals. The dogs had undefined breed and age, from the township region of São Luís-MA. Paraffined slices of the spleen were stained with Hematoxilin and Eosin (H&E); Gomoris ammoniacal Silver, to mark reticular fibers; and the Immunohistochemistry technique of streptavidin peroxidase to detect amastigote forms of Leishmania. The morphometrical analyses were done using the KS300 program and the images analysis system Kontron Elektronic/Carl Zeiss, Germany. The results showed that there is a significant increase in the deposition of collagen fibers in the spleen, compared to control animals, showing significant differences for symptomatic and asymptomatic animals. Positive correlations were found between the presence of the parasite in the tissue and collagen deposition. Symptomatic animals showed higher collagen deposition in the spleen, which can be associated to the high parasitism found in the tissue. The results showed that there is an intense fibrogenesis in the spleen in the canine visceral leishmaniasis, thus being associated to the parasitism of the tissue and the degenerative processes of the disease.

Objetivou-se estudar as alterações da matriz extracelular no baço de cães naturalmente infectados com Leishmania (Leishmania) infantum chagasi correlacionando-as com aspectos clínicos, histopatológicos e parasitológicos. Foram utilizados 18 cães, divididos em três grupos: seis animais não infectados (grupo controle) e doze animais infectados. Todos sem raça e idade definidas, provenientes da região do Município de São Luis-MA. Cortes parafinados do baço foram corados pela Hematoxilina e Eosina (H&E); Prata amoniacal de Gomori, para marcação das fibras reticulares e pela técnica Imuno-histoquímica da estreptoavidina-peroxidase para detecção de formas amastigotas de Leishmania. As análises morfométricas foram feitas utilizando-se o programa KS300 e o sistema de análise de imagens Kontron Elektronic/Carl Zeiss, Germany. Os resultados mostram que há um aumento significativo da deposição de fibras colágenas no baço quando comparadas aos animais controles, revelando diferenças significativas entre os animais sintomáticos e assintomáticos. Encontraram-se correlações positivas entre a presença do parasitismo tecidual e a deposição de colágeno. Os animais sintomáticos apresentaram uma maior deposição de colágeno no baço, que pode estar associada ao maior parasitismo tecidual encontrado. Os resultados demonstram que na leishmaniose visceral canina há uma fibrogênese intensa no baço, sendo esta associada ao parasitismo tecidual e a processos degenerativos decorrentes da doença.

Urolitíase obstrutiva em ovino / Obstructive Urolithiasis in Ovine

Background: Urolithiasis, which is characterized by the presence of stone(s) in the urinary tract, is found in young, male and confined small ruminants. It results from the interaction of several physiological and nutritional factors associated with animal management. The condition is linked to concentrated diets based on grains, with a high phosphorus and magnesium content. The illness becomes important when the stones cause obstruction, which normally occurs in the urethra and which may lead to a rupture of the bladder if the obstruction is not alleviated. In which case, an increase of the abdominal volume is observed, due to the presence of urine in the abdominal cavity. The animal may die from uremia within two to three days, but this period can be longer. Several surgical techniques for the treatment of urolithiasis have been described: amputation of the urethral process with penile transposition, urethrostomy and cystotomy. This study describes a case of obstructive urolithiasis in an ovine, with orchiectomy and penectomy, followed by a perineal urethrostomy. Case: The animal in question is an one-year old half-breed male pet sheep, weighing 11.5 kg and admitted to the Veterinary Medical Teaching Hospital of the Universidade Estadual do Maranhão (UEMA), Brazil. The main complaint was that the animal was not “urinating”. During anamnesis, the owner reported that the condition started approximately seven days ago with anorexia, abdominal distension and difficult urination. At the physical examination, it was observed a slightly increased cardiac frequency, decreased ruminal movements, pale mucous, dehydration, lethargy, distension and intense abdominal pain, urinary bladder filled, strangury, trauma and penile protrusion with necrosis, urine infiltration in the subcutaneous tissue, preputial and testicular edema.[...]

Urolitíase obstrutiva em ovino / Obstructive Urolithiasis in Ovine

Background: Urolithiasis, which is characterized by the presence of stone(s) in the urinary tract, is found in young, male and confined small ruminants. It results from the interaction of several physiological and nutritional factors associated with animal management. The condition is linked to concentrated diets based on grains, with a high phosphorus and magnesium content. The illness becomes important when the stones cause obstruction, which normally occurs in the urethra and which may lead to a rupture of the bladder if the obstruction is not alleviated. In which case, an increase of the abdominal volume is observed, due to the presence of urine in the abdominal cavity. The animal may die from uremia within two to three days, but this period can be longer. Several surgical techniques for the treatment of urolithiasis have been described: amputation of the urethral process with penile transposition, urethrostomy and cystotomy. This study describes a case of obstructive urolithiasis in an ovine, with orchiectomy and penectomy, followed by a perineal urethrostomy. Case: The animal in question is an one-year old half-breed male pet sheep, weighing 11.5 kg and admitted to the Veterinary Medical Teaching Hospital of the Universidade Estadual do Maranhão (UEMA), Brazil. The main complaint was that the animal was not “urinating”. During anamnesis, the owner reported that the condition started approximately seven days ago with anorexia, abdominal distension and difficult urination. At the physical examination, it was observed a slightly increased cardiac frequency, decreased ruminal movements, pale mucous, dehydration, lethargy, distension and intense abdominal pain, urinary bladder filled, strangury, trauma and penile protrusion with necrosis, urine infiltration in the subcutaneous tissue, preputial and testicular edema.[...](AU)