Prevalence and Risk Factors for HTLV-1/2 Infection inRiverside and Rural Populations of the State of Pará.

Human T-lymphotropic viruses 1 and 2 (HTLV-1 and HTLV-2) infection has been described in several Amazonian populations; however, there is still a lack of data on the prevalence of the virus in riparian populations living in rural areas of the state of Pará. The present study aimed to evaluate the prevalence of HTLV-1/2 infection in four riverine communities and one rural area in the state of Pará and to describe the possible risk factors for infection. A total of 907 individuals responded to an epidemiological survey and gave blood samples collected for anti-HTLV-1/2 antibodies by immunoenzymatic assay (EIA). The serum-reactive samples were subjected to confirmation by an in-line assay (Inno-Lia) and by proviral DNA screening using real-time PCR (qPCR). The total prevalence was 0.8% (7/907) for HTLV-1/2 (CI: 0.2-1.3%), with 0.66% HTLV-1 and 0.11% HTLV-2. The prevalence by sex was 0.7% in women (4/565) and 0.9% in men (3/342). Among seropositive patients, 83.3% (5/7) reported being sexually active, and 57.1% (4/7) reported not having the habit of using condoms during their sexual relations. Intrafamily infection was also observed. The results reinforce the need for public policies to prevent and block the spread of HTLV, especially in riparian communities that are subject to difficulties in accessing the Unified Health System (Sistema Único de Saúde/SUS) because infected individuals need clinical monitoring for surveillance and early diagnosis of symptoms associated with HTLV-1.

Changes in the seroprevalence and risk factors between the first and second waves of COVID-19 in a metropolis in the Brazilian Amazon.

In Brazil, the coronavirus disease 2019 (COVID-19) epidemic spread rapidly in a heterogeneous way, mainly due to the different socioeconomic and behavioral characteristics of different regional populations and different evaluation periods. We performed a cross-sectional study including 1,337 individuals (first wave = 736/second wave = 601) after the first two waves of COVID-19 in the city of Belém, the capital of the state of Pará. The detection of IgG anti-SARS-CoV-2 antibodies was performed using an enzyme-linked immunosorbent assay test followed by statistical analysis using the RStudio program. Our results showed an increase in the seroprevalence (first wave= 39.1%/second wave= 50.1%) of anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG antibodies in the population of Belém from the first to the second pandemic wave. Advanced age, primary or secondary education level, lack of social isolation, and a low frequency of protective mask use were considered risk factors for SARS-CoV-2 infection during the first wave compared to the second wave. This study is one of the firsts to provide important information about the dynamics of virus circulation and the groups vulnerable to exposure in the two major periods. Our data emphasize the socioeconomic characteristics of the affected population and that nonpharmacological prevention measures are crucial for combating the pandemic.

HTLV-1/2 in Indigenous Peoples of the Brazilian Amazon: Seroprevalence, Molecular Characterization and Sociobehavioral Factors Related to Risk of Infection.

HTLV-1/2 infection is endemic in Indigenous peoples of the Americas. Its origin is attributed to the migratory flow of Amerindian ancestral peoples. The present study aimed to investigate the seroprevalence of HTLV-1/2 infection in Indigenous peoples of the Brazilian Amazon. A total of 3350 Indigenous people belonging to 15 communities were investigated. The investigation was performed using serological (ELISA), molecular (qPCR) and confirmatory (Western blot and/or Inno-Lia) tests to detect and differentiate the infection. The seroprevalence was 8.3% for HTLV-1/2 infection, with 0.1% of individuals seropositive for HTLV-1 and 8.1% for HTLV-2. The prevalence of infection was statistically higher in women (10.1%) than in men (6.5%) (p = 0.0002). This female predominance was observed in all age groups; in females the prevalence was significant from 41 years old (p < 0.0001) and in males from 51 years old (p < 0.0001). Here, we present a prevalence of HTLV-1/2 among Indigenous peoples of the Brazilian Amazon. The endemic infection in these groups must reflect the different epidemiological profiles observed in these peoples, such as sexual transmission through rejection of condom use, breastfeeding, especially in cases of cross-breastfeeding, and the high rate of pregnancy in the villages.

Metabolic and Ruminal Fluid Markers of Dairy Cows Supplemented with a Combination of Yeast Culture and Hydrolyzed Yeast

Background: In order to reduce the effects of a negative energy balance, some measures have been taken into account in nutritional management during the transition period. The use of yeast, has been a good alternative used to improve the rumen metabolism and helping the adjustment of the microbiotato the new diet. The aim of the study was to evaluate the effects of supplementing a combination of yeast culture and hydrolyzed yeast on the metabolism of dairy cows during the transition period.Materials, Methods & Results: The experiment was conducted in a semi-extensive system, using 20 Holstein cows, divided equally into a control group (CG) and a supplemented group (SG). The SG received 28 g/animal/day of a combination of yeast culture and hydrolyzed yeast from 20 ± 2 days pre-calving until early lactation (18 ± 3 days). Serum concentrations of non-esterified fatty acids (NEFA), albumin and urea were determined at calving, and for three time points during the early postpartum period and three time points during the early lactation period. Regarding energy metabolism, prepartum concentrations of NEFA were higher than the physiological standard in both groups. However, NEFA, albumin and urea decreased during the early postpartum period in the supplemented animals and could be attributed to the yeast in enhancing ruminal microorganisms cellulolytic capacity, increasing fibre d

Metabolic and Ruminal Fluid Markers of Dairy Cows Supplemented with a Combination of Yeast Culture and Hydrolyzed Yeast

Background: In order to reduce the effects of a negative energy balance, some measures have been taken into account in nutritional management during the transition period. The use of yeast, has been a good alternative used to improve the rumen metabolism and helping the adjustment of the microbiotato the new diet. The aim of the study was to evaluate the effects of supplementing a combination of yeast culture and hydrolyzed yeast on the metabolism of dairy cows during the transition period.Materials, Methods & Results: The experiment was conducted in a semi-extensive system, using 20 Holstein cows, divided equally into a control group (CG) and a supplemented group (SG). The SG received 28 g/animal/day of a combination of yeast culture and hydrolyzed yeast from 20 ± 2 days pre-calving until early lactation (18 ± 3 days). Serum concentrations of non-esterified fatty acids (NEFA), albumin and urea were determined at calving, and for three time points during the early postpartum period and three time points during the early lactation period. Regarding energy metabolism, prepartum concentrations of NEFA were higher than the physiological standard in both groups. However, NEFA, albumin and urea decreased during the early postpartum period in the supplemented animals and could be attributed to the yeast in enhancing ruminal microorganisms cellulolytic capacity, increasing fibre d

Metabolic and Ruminal Fluid Markers of Dairy Cows Supplemented with a Combination of Yeast Culture and Hydrolyzed Yeast

Background: In order to reduce the effects of a negative energy balance, some measures have been taken into account in nutritional management during the transition period. The use of yeast, has been a good alternative used to improve the rumen metabolism and helping the adjustment of the microbiotato the new diet. The aim of the study was to evaluate the effects of supplementing a combination of yeast culture and hydrolyzed yeast on the metabolism of dairy cows during the transition period.Materials, Methods & Results: The experiment was conducted in a semi-extensive system, using 20 Holstein cows, divided equally into a control group (CG) and a supplemented group (SG). The SG received 28 g/animal/day of a combination of yeast culture and hydrolyzed yeast from 20 ± 2 days pre-calving until early lactation (18 ± 3 days). Serum concentrations of non-esterified fatty acids (NEFA), albumin and urea were determined at calving, and for three time points during the early postpartum period and three time points during the early lactation period. Regarding energy metabolism, prepartum concentrations of NEFA were higher than the physiological standard in both groups. However, NEFA, albumin and urea decreased during the early postpartum period in the supplemented animals and could be attributed to the yeast in enhancing ruminal microorganisms cellulolytic capacity, increasing fibre d

Metabolic and Ruminal Fluid Markers of Dairy Cows Supplemented with a Combination of Yeast Culture and Hydrolyzed Yeast

Background: In order to reduce the effects of a negative energy balance, some measures have been taken into account in nutritional management during the transition period. The use of yeast, has been a good alternative used to improve the rumen metabolism and helping the adjustment of the microbiotato the new diet. The aim of the study was to evaluate the effects of supplementing a combination of yeast culture and hydrolyzed yeast on the metabolism of dairy cows during the transition period.Materials, Methods & Results: The experiment was conducted in a semi-extensive system, using 20 Holstein cows, divided equally into a control group (CG) and a supplemented group (SG). The SG received 28 g/animal/day of a combination of yeast culture and hydrolyzed yeast from 20 ± 2 days pre-calving until early lactation (18 ± 3 days). Serum concentrations of non-esterified fatty acids (NEFA), albumin and urea were determined at calving, and for three time points during the early postpartum period and three time points during the early lactation period. Regarding energy metabolism, prepartum concentrations of NEFA were higher than the physiological standard in both groups. However, NEFA, albumin and urea decreased during the early postpartum period in the supplemented animals and could be attributed to the yeast in enhancing ruminal microorganisms cellulolytic capacity, increasing fibre d

Metabolic and Ruminal Fluid Markers of Dairy Cows Supplemented with a Combination of Yeast Culture and Hydrolyzed Yeast

Background: In order to reduce the effects of a negative energy balance, some measures have been taken into account in nutritional management during the transition period. The use of yeast, has been a good alternative used to improve the rumen metabolism and helping the adjustment of the microbiotato the new diet. The aim of the study was to evaluate the effects of supplementing a combination of yeast culture and hydrolyzed yeast on the metabolism of dairy cows during the transition period.Materials, Methods & Results: The experiment was conducted in a semi-extensive system, using 20 Holstein cows, divided equally into a control group (CG) and a supplemented group (SG). The SG received 28 g/animal/day of a combination of yeast culture and hydrolyzed yeast from 20 ± 2 days pre-calving until early lactation (18 ± 3 days). Serum concentrations of non-esterified fatty acids (NEFA), albumin and urea were determined at calving, and for three time points during the early postpartum period and three time points during the early lactation period. Regarding energy metabolism, prepartum concentrations of NEFA were higher than the physiological standard in both groups. However, NEFA, albumin and urea decreased during the early postpartum period in the supplemented animals and could be attributed to the yeast in enhancing ruminal microorganisms cellulolytic capacity, increasing fibre d

Metabolic and Ruminal Fluid Markers of Dairy Cows Supplemented with a Combination of Yeast Culture and Hydrolyzed Yeast

Background: In order to reduce the effects of a negative energy balance, some measures have been taken into account in nutritional management during the transition period. The use of yeast, has been a good alternative used to improve the rumen metabolism and helping the adjustment of the microbiotato the new diet. The aim of the study was to evaluate the effects of supplementing a combination of yeast culture and hydrolyzed yeast on the metabolism of dairy cows during the transition period.Materials, Methods & Results: The experiment was conducted in a semi-extensive system, using 20 Holstein cows, divided equally into a control group (CG) and a supplemented group (SG). The SG received 28 g/animal/day of a combination of yeast culture and hydrolyzed yeast from 20 ± 2 days pre-calving until early lactation (18 ± 3 days). Serum concentrations of non-esterified fatty acids (NEFA), albumin and urea were determined at calving, and for three time points during the early postpartum period and three time points during the early lactation period. Regarding energy metabolism, prepartum concentrations of NEFA were higher than the physiological standard in both groups. However, NEFA, albumin and urea decreased during the early postpartum period in the supplemented animals and could be attributed to the yeast in enhancing ruminal microorganisms cellulolytic capacity, increasing fibre d