RESUMEN
We previously hypothesized that depressive and manic states may be consecutive presentations of the same underlying neuronal plasticity, and that moderate impairments in neuronal plasticity cause depressive states while further impairment to neuronal plasticity causes manic states. Psychopathological or biological relationships between bipolar disorder and schizophrenia have also been revealed. Therefore, in addition to depressive and manic states, psychosis may also be considered a manifestation resulting from additional impairments to neuronal plasticity. In the present manuscript, we hypothesize that moderate and more severe impairments to neuronal plasticity cause depressive and manic states, respectively, and that more serious impairments to neuronal plasticity cause psychosis. Many studies have suggested that impairments in neuronal plasticity contribute to schizophrenia and other mental disorders with psychotic features, and that the impairment of neuronal plasticity in schizophrenia is more severe than that in bipolar disorder. Therefore, we hypothesize more specifically that impairments in neuronal plasticity may be more severe in the order of the cases featuring psychosis, mania, and depression. This progression notably overlaps with the arrangement of schizophrenia, bipolar disorder, and depressive disorder in the DSM-5. Psychotic symptoms are thought to appear further towards the base of the psychopathological hierarchy than are manic or depressive symptoms. If impairments to neuronal plasticity contribute to this psychopathological hierarchy, as we contest that they do, our hypothesis may serve as a bridge between clinical psychopathology, diagnosis, and biological psychiatry.
Asunto(s)
Trastorno Bipolar/diagnóstico , Plasticidad Neuronal , Trastornos Psicóticos/diagnóstico , Esquizofrenia/diagnóstico , Síntomas Afectivos , Psiquiatría Biológica , Factor Neurotrófico Derivado del Encéfalo/metabolismo , Depresión/diagnóstico , Progresión de la Enfermedad , Humanos , Modelos PsicológicosRESUMEN
OBJECTIVES: We evaluated the neuroprotective effect of chronically or acutely administered lithium against hypoxia in several brain regions. Furthermore, we investigated the contribution of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and cAMP response element binding protein (CREB) to the neuroprotective effect of lithium. METHODS: Brain slices were prepared from rats that had been treated chronically or acutely with lithium. The cerebral glucose metabolic rate (CMRglc) before and after hypoxia loading to brain slices was measured using the dynamic positron autoradiography technique with [(18)F]2-fluoro-2-deoxy-D-glucose. The changes of expression of proteins were investigated using Western blot analysis. RESULTS: Before hypoxia loading, the CMRglc did not differ between the lithium-treated and untreated groups. After hypoxia loading, the CMRglc of the untreated group was significantly lower than that before hypoxia loading. However, the CMRglc of the chronic lithium treatment group recovered in the frontal cortex, caudate putamen, hippocampus and cerebellum, but not in the thalamus. In contrast, the CMRglc of the acute lithium treatment group did not recover in any analyzed brain regions. After chronic lithium treatment, the levels of expression of BDNF and phospho-CREB were higher than those of untreated rats in the frontal cortex, but not in the thalamus. However, the expression of NGF did not change in the frontal cortex and thalamus. CONCLUSIONS: These results demonstrated that lithium was neuroprotective against hypoxia only after chronic treatment and only in specific brain regions, and that CREB and BDNF might contribute to this effect.
Asunto(s)
Encéfalo/efectos de los fármacos , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/metabolismo , Hipoxia , Compuestos de Litio/uso terapéutico , Fármacos Neuroprotectores/uso terapéutico , Regulación hacia Arriba/efectos de los fármacos , Animales , Encéfalo/diagnóstico por imagen , Encéfalo/patología , Mapeo Encefálico , Factor Neurotrófico Derivado del Encéfalo/metabolismo , Esquema de Medicación , Fluorodesoxiglucosa F18/metabolismo , Hipoxia/tratamiento farmacológico , Hipoxia/patología , Hipoxia/fisiopatología , Técnicas In Vitro , Insulina/farmacología , Compuestos de Litio/farmacología , Masculino , Fármacos Neuroprotectores/farmacología , Tomografía de Emisión de Positrones , Ratas , Ratas Wistar , Factores de TiempoRESUMEN
1-Methyl-4-phenylpyridinium (MPP(+)) was added directly to fresh rat brain slices and the dynamic changes in the cerebral glucose metabolic rate (CMRglc) were serially and two-dimensionally measured with [(18)F]2-fluoro-2-deoxy-D-glucose as a tracer. MPP(+) dose-dependently increased CMRglc, reflecting enhanced glycolysis compensating for the decrease in aerobic metabolism. While the CMRglc enhancement induced by MPP(+) (<10 microM) was restricted to the striatum, MPP(+) (>or=10 microM) induced a significant CMRglc enhancement in all brain regions. MPP(+) at high concentration (1 mM) eventually initiated rapid metabolic collapse, with failure to sustain anaerobic glycolysis.
Asunto(s)
1-Metil-4-fenilpiridinio/toxicidad , Mapeo Encefálico , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Glucosa/metabolismo , Herbicidas/toxicidad , Anaerobiosis/efectos de los fármacos , Anaerobiosis/fisiología , Animales , Encéfalo/fisiología , Mapeo Encefálico/métodos , Glucosa/deficiencia , Glucólisis/efectos de los fármacos , Glucólisis/fisiología , Masculino , Técnicas de Cultivo de Órganos , Ratas , Ratas Wistar , Factores de TiempoRESUMEN
To explore the aetiology of pathological laughing, a 65-year-old woman with pathological laughing was examined by 3-T functional magnetic resonance imaging (fMRI) before and after treatment with drugs. Here, we report that the patient consistently showed exaggerated pontine activation during the performance of three tasks before treatment, whereas abnormal pontine activation was no longer found after successful treatment with the selective serotonin reuptake inhibitor, paroxetine. Our findings in this first fMRI study of pathological laughing suggest that serotonergic replacement decreases the aberrant activity in a circuit that involves the pons.
Asunto(s)
Síntomas Afectivos/fisiopatología , Risa , Puente/fisiopatología , Síntomas Afectivos/patología , Anciano , Femenino , Humanos , Imagen por Resonancia Magnética , Paroxetina/farmacología , Puente/patología , Inhibidores Selectivos de la Recaptación de Serotonina/farmacología , Análisis y Desempeño de TareasRESUMEN
We examined the induction of hypoxic tolerance after hypoxic preconditioning in the frontal cortex, caudate putamen and thalamus using the dynamic positron autoradiography technique and [18F]2-fluoro-2-deoxy-D-glucose with rat brain slices. Hypoxic tolerance induction was confirmed in the frontal cortex, but not in the caudate putamen and thalamus. Next, we compared the gene expression in the frontal cortex and caudate putamen using the ATLAS Rat Stress Array, and found that the expression of 150-kDa oxygen-regulated protein and mitochondrial heat shock protein 70 as stress proteins, and copper-zinc-containing superoxide dismutase and manganese-containing superoxide dismutase as antioxidant enzymes was elevated only in the frontal cortex. These results suggest that the induction of hypoxic tolerance after hypoxic preconditioning is region-specific, and stress proteins and antioxidant enzymes participate in this phenomenon.
Asunto(s)
Antioxidantes/metabolismo , Encéfalo/metabolismo , Hipoxia-Isquemia Encefálica/metabolismo , Precondicionamiento Isquémico , Proteínas del Tejido Nervioso/genética , Estrés Oxidativo/fisiología , Animales , Encéfalo/irrigación sanguínea , Encéfalo/fisiopatología , Modelos Animales de Enfermedad , Lóbulo Frontal/irrigación sanguínea , Lóbulo Frontal/metabolismo , Lóbulo Frontal/fisiopatología , Regulación Enzimológica de la Expresión Génica/genética , Proteínas HSP70 de Choque Térmico/genética , Hipoxia-Isquemia Encefálica/genética , Hipoxia-Isquemia Encefálica/fisiopatología , Masculino , Neostriado/irrigación sanguínea , Neostriado/metabolismo , Neostriado/fisiopatología , Análisis de Secuencia por Matrices de Oligonucleótidos , Proteínas/genética , Ratas , Ratas Wistar , Superóxido Dismutasa/genética , Regulación hacia Arriba/genéticaRESUMEN
Atopic dermatitis (AD) is a chronic inflammatory skin disease of unknown etiology. To examine the involvement of impaired homeostasis of oxygen/nitrogen radicals in childhood AD, we compared the levels of urinary 8-hydroxy-2'-deoxyguanosine (marker of oxidative stress), nitrite/nitrate (marker of nitric oxide synthesis) and selenium (marker of selenium store) in 27 children with AD to those of 25 healthy control children. Urinary 8-hydroxy-2'-deoxyguanosine was significantly higher and nitrite/nitrate levels were significantly lower in patients with AD than in the control. Urinary selenium levels were similar in both groups. Our findings suggest that impaired homeostasis of oxygen/nitrogen radicals and increased oxidative stress are involved in the pathophysiology of childhood AD, and indicate that suppression of oxidative stress might be a potentially useful strategy for the treatment of AD.
Asunto(s)
Desoxiguanosina/orina , Dermatitis Atópica/metabolismo , Estrés Oxidativo , 8-Hidroxi-2'-Desoxicoguanosina , Adolescente , Niño , Preescolar , Desoxiguanosina/análogos & derivados , Ensayo de Inmunoadsorción Enzimática , Femenino , Homeostasis , Humanos , Masculino , Nitratos/orina , Nitritos/orina , Selenio/orinaRESUMEN
To reveal the metabolic fate of acetate in neoplasms that may characterize the accumulation patterns of [1-(11)C]acetate in tumors depicted by positron emission tomography. Four tumor cell lines (LS174T, RPMI2650, A2780, and A375) and fibroblasts in growing and resting states were used. In uptake experiments, cells were incubated with[1-(14)C]acetate for 40 min. [(14)C]CO(2) was measured in the tight-air chamber, and the metabolites in cells were identified by thin layer chromatography and paper chromatography. The glucose metabolic rate of each cell line was measured with [2,6-(3)H]2-deoxy-glucose (DG), and the growth activity of each cell line was estimated by measuring the incorporation of [(3)H]methyl thymidine into DNA. Compared with resting fibroblasts, all four tumor cell lines showed higher accumulation of (14)C activity from [1-(14)C]acetate. These tumor-to-normal ratios of [1-(14)C]acetate were larger than those of DG. Tumor cells incorporated (14)C activity into the lipid-soluble fraction, mostly of phosphatidylcholine and neutral lipids, more prominently than did fibroblasts. The lipid-soluble fraction of (14)C accumulation in cells showed a positive correlation with growth activity, whereas the water-soluble and CO(2) fractions did not. These findings suggest that the high tumor-to-normal ratio of [1-(14)C]acetate is mainly due to the enhanced lipid synthesis, which reflects the high growth activity of neoplasms. This in vitro study suggests that [1-(11)C]acetate is appropriate for estimating the growth activity of tumor cells.
Asunto(s)
Acetatos/metabolismo , División Celular/fisiología , Acetilcoenzima A/metabolismo , Radioisótopos de Carbono , Neoplasias del Colon , Desoxiglucosa/farmacocinética , Femenino , Fibroblastos/enzimología , Humanos , Cinética , Melanoma , Neoplasias Nasales , Neoplasias Ováricas , Tomografía Computarizada de Emisión , Tritio , Células Tumorales CultivadasRESUMEN
To investigate age-related changes in cerebral energy production, we compared senescence-accelerated prone mice (SAMP8) as an animal model of accelerated aging and senescence-accelerated resistant mice (SAMR1) as a control. Considering that the cerebral glucose metabolic rate (CMRglc) at the time of O(2) deprivation and 2,4-dinitrophenol (DNP) loading would reflect anaerobic glycolytic capacity and mitochondrial function, respectively, we investigated dynamic changes in CMRglc before and after loading with these perturbations. Fresh brain slices were incubated with [(18)F]2-fluoro-2-deoxy-D-glucose ([(18)F]FDG) in oxygenated Krebs-Ringer solution at 36 degrees C, and serial two-dimensional time-resolved images of [(18)F]FDG uptake in these slices were obtained on the imaging plates. The fractional rate constant (=k(3)*) of [(18)F]FDG proportional to the CMRglc was evaluated by applying the Gjedde-Patlak graphical method to the image data. The k(3)* value before the hypoxic perturbation in all of the brain sites analyzed was higher in SAMP8 than SAMR1 in both the 2- and 10-month-old groups. With O(2) deprivation, k(3)* values were higher without site specificity in the 2-month-old SAMP8 than in 2-month-old SAMR1, whereas in 10-month-old mice, there was no significant difference between the two groups. In contrast, with DNP loading, while no significant difference was noted between 2-month-old SAMP8 and 2-month-old SAMR1, in 10-month-old mice, the SAMP8 group showed lower values in certain regions than SAMR1 mice. These results suggest that in the brain tissue of SAMP8, a marked transient enhancement of anaerobic glycolytic capacity in the 2-month-olds and a decrease in mitochondrial function in the subsequent period occur, as a result of which glucose metabolism appears to be enhanced in both the 2- and 10-month-old groups compared to SAMR1 mice.
Asunto(s)
Envejecimiento/fisiología , Encéfalo/metabolismo , Glucosa/metabolismo , Factores de Edad , Animales , Autorradiografía , Fraccionamiento Celular , Técnicas de Cultivo , Electrones , Masculino , Ratones , Mitocondrias/fisiología , Oxígeno/metabolismoRESUMEN
Seven-day-old rat brain slices were incubated at 36C in oxygenated Krebs-Ringer solution containing [(18)F]2-fluoro-2-deoxy-D-glucose ([(18)F]FDG), and serial two-dimensional time-resolved images of [(18)F]FDG uptake by the slices were obtained. The Gjedde-Patlak graphical method was applied to the image data, and the duration limit of hypoxia loading that allowed recovery of the fractional rate constant (k3*) of [(18)F]FDG (proportional to the cerebral glucose metabolic rate) after hypoxia loading to the unloaded control level was 50 min, and MK-801 as an N-methyl-D-aspartate antagonist had neuroprotective effects, but PBN as a free radical scavenger was ineffective. In our previous study in adult (7-week-old) rat brains [Murata et al., Exp Neurol 2000, 164:269-279], the limit of the hypoxia loading time was 20 min, and both MK-801 and PBN were effective. In the immature rat brains, the ratio of aerobic glucose metabolism to the total glucose metabolism was low compared with the adult rat brains, suggesting only a slight involvement of free radicals in hypoxic neurotoxicity. These data suggest that the higher resistance of immature brains to hypoxia compared to that of adult brains is attributable to a lower involvement of free radicals due to a lower aerobic glucose metabolic rate.
Asunto(s)
Encéfalo/diagnóstico por imagen , Encéfalo/crecimiento & desarrollo , Metabolismo Energético/fisiología , Radicales Libres/metabolismo , Glucosa/metabolismo , Hipoxia Encefálica/diagnóstico por imagen , Neurotoxinas/metabolismo , Envejecimiento/metabolismo , Animales , Animales Recién Nacidos , Encéfalo/fisiopatología , Depuradores de Radicales Libres/farmacología , Masculino , Técnicas de Cultivo de Órganos , Cintigrafía , Ratas , Ratas Sprague-DawleyRESUMEN
Using a positron autoradiography technique, dynamic changes in the cerebral glucose metabolic rate (CMRglc) induced by hypoxia/reoxygenation were investigated in living brain slices. After incubating fresh rat brain slices (300 microm thick) with [(18)F]2-fluoro-2-deoxy-D-glucose ([(18)F]FDG) in oxygenated Krebs-Ringer solution at 36 degrees C, serial two-dimensional time-resolved images of [(18)F]FDG uptake in the slices were obtained on imaging plates. As compared to the unloaded control values, with hypoxia-loading [(18)F]FDG uptake increased markedly, suggesting enhanced glycolysis. The net influx constant (K) of [(18)F]FDG at pre-hypoxia-loading and after reoxygenation with loading hypoxia for various periods of time was quantitatively evaluated by applying the Patlak graphical method to the image data. Regardless of the brain region, with hypoxia of =10-min duration, the K value returned to the preloading level, whereas with hypoxia of >/=20 min duration only partial or no recovery was seen, indicating irreversible neuronal damage. The 30-min administration of either N-methyl-D-aspartate (NMDA)/non-NMDA antagonist or a free radical scavenger at the same time as reoxygenation after 20 min hypoxia showed a neuroprotective effect inhibiting the decrease in the post-hypoxia-loading K value. In contrast, no such neuroprotective effect was evident with administration of either of these agents only during hypoxia loading, possibly indicating that immediately after reoxygenation neuronal damage was induced mediated by excitatory amino acids and free radicals in tandem. These results demonstrate that serial quantitative evaluation of CMRglc using this technique may be of use in investigating the brain tissue injury associated with hypoxia/reoxygenation as well as clarifying the underlying mechanisms and protective effect of various drugs against such injury.
Asunto(s)
Antagonistas de Aminoácidos Excitadores/farmacología , Depuradores de Radicales Libres/farmacología , Glucosa/metabolismo , Hipoxia Encefálica/metabolismo , Fármacos Neuroprotectores/farmacología , Oxazinas , Daño por Reperfusión/prevención & control , Xantenos , 6-Ciano 7-nitroquinoxalina 2,3-diona/farmacología , Animales , Autorradiografía/métodos , Hipoxia de la Célula/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Colorantes , Óxidos N-Cíclicos , Maleato de Dizocilpina/farmacología , Fluorodesoxiglucosa F18/farmacocinética , Glucólisis/efectos de los fármacos , Hipoxia Encefálica/patología , Técnicas In Vitro , Ácido Láctico/metabolismo , Masculino , Neuronas/citología , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Óxidos de Nitrógeno/farmacología , Ratas , Receptores de N-Metil-D-Aspartato/antagonistas & inhibidoresRESUMEN
Fresh rat brain slices were incubated with [18F]2-fluoro-2-deoxy-D-glucose ([18F]FDG) in oxygenated Krebs-Ringer solution at 36 degrees C, and serial two-dimensional time-resolved images of [18F]FDG uptake in the slices were obtained on imaging plates. The fractional rate constant of [18F]FDG (proportional to the cerebral glucose metabolic rate) from pre-loading of ischemia (O(2) and glucose deprivation)/hypoxia (O(2) deprivation) to the reperfused/reoxygenated post-loading phase was quantitatively evaluated by applying the Gjedde-Patlak graphical method to the image data. Against ischemia an N-methyl-D-aspartate antagonist and hypothermia, but not a free radical scavenger, showed a protective effect when administered during ischemia, whereas no such effect was achieved with any of the above agents when administered after reperfusion. Against hypoxia, there was no protective effect with any of the above agents when administered during hypoxia, although an effect was noted with each when administered after reoxygenation. Excitatory amino acids during ischemia loading were found to be the main factor in the neuronal damage associated with ischemia, while in hypoxia, excitatory amino acids working in tandem with free radicals immediately after reoxygenation were implicated.
Asunto(s)
Isquemia Encefálica/metabolismo , Encéfalo/metabolismo , Glucosa/metabolismo , Ácido Glutámico/metabolismo , Animales , Encéfalo/efectos de los fármacos , Óxidos N-Cíclicos , Maleato de Dizocilpina/farmacología , Fluorodesoxiglucosa F18/metabolismo , Ácido Glutámico/efectos de los fármacos , Hipotermia/metabolismo , Hipoxia Encefálica/fisiopatología , Masculino , Fármacos Neuroprotectores/farmacología , Óxidos de Nitrógeno/farmacología , Radiofármacos/metabolismo , Ratas , Ratas Sprague-DawleyRESUMEN
Dynamic changes in the regional cerebral glucose metabolic rate induced by hypoxia/reoxygenation or ischemia/reperfusion were investigated with a positron autoradiography technique. Fresh rat brain slices were incubated with [18F]2-fluoro-2-deoxy-D-glucose ([18F]FDG) in oxygenated Krebs-Ringer solution at 36 degrees C, and serial two-dimensional time-resolved images of [18F]FDG uptake in the slices were obtained. In the case of loading hypoxia (oxygen deprivation)/pseudoischemia (oxygen and glucose deprivation) for various periods of time, the net influx constant (K) of [18F]FDG at preloading and after reoxygenation/pseudoreperfusion (post-loading) was quantitatively evaluated by applying the Patlak graphical method to the image data. Regardless of the brain region, with hypoxia lasting > or =20 minutes, the postloading K value was decreased compared with the unloaded control, whereas with pseudoischemia of < or =40 minutes, approximately the same level as the unloaded control was maintained. Next, the neuroprotective effect against hypoxia/pseudoischemia loading induced by the addition of a free radical scavenger or an N-methyl-D-aspartate (NMDA) antagonist was assessed by determining whether a decrease in the postloading K value was prevented. Whereas with 20-minute hypoxia, both agents exhibited a neuroprotective effect, in the case of 50-minute pseudoischemia, only the NMDA antagonist did so, with the free radical scavenger being ineffective. These results demonstrate that hypoxia causes irreversible neuronal damage within a shorter period than ischemia, with both free radicals and glutamate suggested to be involved in tandem in the neurotoxicity induced by hypoxia, whereas glutamate alone is involved in ischemic neurotoxicity.
Asunto(s)
Glucosa/metabolismo , Ácido Glutámico/metabolismo , Hipoxia Encefálica/fisiopatología , Ataque Isquémico Transitorio/fisiopatología , Animales , Autorradiografía/métodos , Encéfalo/irrigación sanguínea , Encéfalo/metabolismo , Circulación Cerebrovascular/efectos de los fármacos , Óxidos N-Cíclicos , Maleato de Dizocilpina/farmacología , Antagonistas de Aminoácidos Excitadores/farmacología , Fluorodesoxiglucosa F18 , Radicales Libres/metabolismo , Hipoxia Encefálica/diagnóstico por imagen , Hipoxia Encefálica/metabolismo , Técnicas In Vitro , Ataque Isquémico Transitorio/diagnóstico por imagen , Ataque Isquémico Transitorio/metabolismo , Masculino , Neurotoxinas/metabolismo , Óxidos de Nitrógeno/farmacología , Oxígeno/metabolismo , Cintigrafía , Ratas , Ratas Sprague-Dawley , Daño por Reperfusión/diagnóstico por imagen , Daño por Reperfusión/metabolismo , Daño por Reperfusión/fisiopatología , Marcadores de SpinRESUMEN
Dynamic changes in the cerebral glucose metabolic rate (CMRglc) before and after thiamine replenishment were investigated in living brain slices obtained from pyrithiamine-treated (PT) and pair-fed control rats by use of a positron autoradiography technique. Fresh rat brain slices (300 microm thick) were incubated with [18F]2-fluoro-2-deoxy-D-glucose ([18F]FDG) in oxygenated Krebs-Ringer solution at 36 degrees C, during which serial two-dimensional images of [18F]FDG uptake in the slices were constructed on the imaging plates. The net influx constant (=K) of [18F]FDG was determined by a Patlak graphical method of the image data. Prior to thiamine pyrophosphate (TPP)-loading, the K value in the neurologically symptomatic PT was higher in all brain regions except the thalamus and mammillary body than the control, suggesting compensatory enhanced glycolysis. The rapid decrease in this heightened net influx constant immediately after TPP-loading was surmised to be due to activation of pyruvate oxidation with lactate as the substrate, with this inhibiting the glycolysis. From > or = 150 min after TPP-loading, the K value continued to show low values in the thalamus and mammillary body, which are regarded as the responsible sites for Korsakoff syndrome, whereas in all other sites recovery to control values was observed. These findings suggest that using this technique the quantitative evaluation of serial local changes in CMRglc from thiamine deficiency to after its replenishment may be useful in elucidating the pathophysiology and prognosis of Wernicke's encephalopathy.
Asunto(s)
Encéfalo/metabolismo , Glucosa/metabolismo , Deficiencia de Tiamina/metabolismo , Animales , Autorradiografía , Fluorodesoxiglucosa F18/farmacocinética , Masculino , Radiofármacos/farmacocinética , Ratas , Encefalopatía de Wernicke/metabolismoRESUMEN
Fresh rat brain slices were incubated with 2-deoxy-2-[18F]-fluoro-D-glucose ([18F]FDG) in oxygenated Krebs-Ringer solution at 36 degrees C, and serial two-dimensional time-resolved images of [18F]FDG uptake were obtained from these specimens on imaging plates. The fractional rate constant (= k3*) of [18F]FDG proportional to the cerebral glucose metabolic rate (CMRglc) was evaluated by applying the Gjedde-Patlak graphical method to the image data. With hypoxia loading (oxygen deprivation) or glucose metabolism inhibitors acting on oxidative phosphorylation, the k3* value increased dramatically suggesting enhanced glycolysis. After relieving hypoxia < or = 10-min, the k3* value returned to the pre-loading level. In contrast, with > or = 20-min hypoxia only partial or no recovery was observed, indicating that irreversible neuronal damage had been induced. However, after loading with tetrodotoxin (TTX), the k3* value also decreased but returned to the pre-loading level even after 70-min TTX-loading, reflecting a transient inhibition of neuronal activity. This technique provides a new means of quantifying dynamic changes in the regional CMRglc in living brain slices in response to various interventions such as hypoxia and neurotoxic chemical-loading as well as determining the viability and prognosis of brain tissues.
Asunto(s)
Autorradiografía/métodos , Encéfalo/metabolismo , Glucosa/metabolismo , Hipoxia/metabolismo , Animales , Autorradiografía/instrumentación , Encéfalo/efectos de los fármacos , Medios de Cultivo , Electrones , Fluorodesoxiglucosa F18/farmacocinética , Técnicas In Vitro , Lactatos/análisis , Masculino , Radiofármacos/farmacocinética , Ratas , Ratas Sprague-Dawley , Tetrodotoxina/farmacologíaRESUMEN
To demonstrate the preference of lactate over glucose as an energy substrate in normal brain tissue under normoxic condition, the dynamic changes in glucose uptake by lactate loading were investigated in living rat brain slices using a positron autoradiography technique. Fresh rat brain slices were incubated with [18F]2-fluoro-2-deoxy-D-glucose ([18F]FDG) in oxygenated Krebs-Ringer solution containing 10 mM glucose at 36 degrees C. During incubation, serial two-dimensional imaging of [18F]FDG uptake in the slices was constructed on the imaging plates. Lactate loading (20 mM) reversibly suppressed the [18F]FDG accumulation up to 80 min. Compared with the pre-loading and the unloaded control values, [18F]FDG uptake was suppressed to 25-45% in cerebral regions and 6-7% in cerebellum. The lactate concentration in the surrounding medium decreased after lactate loading. Hence brain tissue preferentially uses lactate over glucose under normoxic and euglycemic condition.
Asunto(s)
Encéfalo/metabolismo , Glucosa/metabolismo , Ácido Láctico/metabolismo , Animales , Cerebelo/metabolismo , Cuerpo Estriado/metabolismo , Electrones , Fluorodesoxiglucosa F18/farmacocinética , Lóbulo Frontal/metabolismo , Hipocampo/metabolismo , Técnicas In Vitro , Masculino , Radiofármacos/farmacocinética , Ratas , Ratas Sprague-Dawley , Tálamo/metabolismo , Factores de TiempoRESUMEN
The positron-emitting radionuclide 18F was used to label 2-fluoro-2-deoxy-D-glucose producing [18F]2-fluoro-2-deoxy-D-glucose ([18F]FDG), and subsequently applied to sagittally-sectioned brain slices of the rat to evaluate the activity of neurons for up to 7 h in living brain slices. The amount of [18F]FDG uptake, which is proportional to the activity of neurons, was monitored every 20 min in five representative brain regions: frontal cortex, caudate-putamen, thalamus, hippocampus and cerebellum. The uptake of [18F]FDG linearly increased with time in these areas, showing constant glucose utilization. The rate of uptake was reversibly decreased by tetrodotoxin (TTX) regardless of brain region, but some uptake was insensitive to TTX. There was a tendency for the uptake to be decreased in Ca2+-free, 5 mM Mg2+ (2 mM EGTA) solution, suggesting some remaining functional synapses. Thus in sagittally-sectioned brain slices, most glucose metabolism is dedicated to neuronal firings and some metabolism to synaptic activities and to other functions of neurons and glial cells. When Cd2+ was applied to brain slices at 0.1-1 mM, the curve of [18F]FDG uptake irreversibly declined, indicating its toxic effect rather than its blocking action of transmitter release at synapses. The cerebellum was the most sensitive to Cd2+, and the caudate-putamen was the least sensitive. The present method, therefore, can be also used as a rapid examination system for checking neurotoxicity of substances.