[Radio wave and cryodestruction in the complex therapy of cervical lesions].

We have determined the effectiveness of cryodestruction and radio wave surgery during the treatment of background diseases of cervical lesions. To 52 patients with background diseases of cervical lesions the radio wave surgery has been performed and for 81 patients cryodestruction has been carried out, 65 patients got laser, anti-virus and immunomodulating therapy after cryosurgical treatment. The usage of radio wave surgery and cryodestruction helps to avoid unpleasant postoperative consequences such as pain, edema, infection and postoperative shock from loss of blood, which quite often arise after application of traditional methods of surgical operation. The advantage of cryodestruction and radio wave surgery at treatment of background diseases of cervical lesions in comparison with other invasive methods of treatment of given pathology is the absence of scars and preservation of cervical lesions' reproductive function. During the radio wave surgery operation the depth of destruction of tissues are being controlled by the adjustment of the device's capacity, which in our opinion is an advantage of radio wave method of treatment of cervical lesions' pathology in comparison with the method of cryodestruction.

[Female reproductive function and immune status after M. Stark's modified cesarean section].

With the aim of studying the causes of peculiarities of female reproductive function and secondary sterility as a result of 100 cesarean sections by M. Stark's modified method, we have attempted to determine the immune status of organisms of women in post-operational period, in post-operational purulent septic complications and in post-operational process of uterus involution. As a result of hysteroscopy and metrosalpingographic tests it has been established that a secondary sterility after cesarean section carried out according to M. Stark modification is manifested 2.5 times less than after traditional method of cesarean section which widely used nowadays.

Modification in the inherent mode of allelic replication in lymphocytes of patients suffering from renal cell carcinoma: a novel genetic alteration associated with malignancy.

Using fluorescence in situ hybridization (FISH) to interphase nuclei, we examined the replication timing of 1 allele relative to its counterpart in PHA-stimulated peripheral blood lymphocytes of normal subjects and patients suffering from a solid tumor (renal cell carcinoma). In the FISH assay, an unreplicated DNA sequence is identified by a single dot-like hybridization signal, whereas a replicated region gives rise to a duplicated, bipartite signal. Accordingly, lymphocytes of normal individuals show 2 patterns of allelic replication: (i) synchronized replication of allelic counterparts, as exemplified by the biallelically expressed loci TP53 and D21S55; and (ii) non-synchronized replication of allelic partners, as exemplified by the early and late replicating alleles of GABRB3, an imprinted locus subjected to monoallelic expression. However, when present in lymphocytes of the cancer patients, all 3 loci change their replication mode: alleles of TP53 and D21S55 become asynchronous, whereas the early replicating allele of GABRB3 delays replication, leading to relaxation in the imprinted mode of replication. Based on the tight relationship between temporal order of allelic replication and allelic mode of expression, the modified order of allelic replication observed in nonmalignant cells of individuals diagnosed with cancer represents a novel genetic alteration associated with malignancy. This alteration detected by simple cytogenetic means, applied to peripheral blood lymphocytes, offers a potential test for cancer identification. Genes Chromosomes Cancer 27:270-277, 2000.